In vitro antioxidant, DNA-damaged protection and antiproliferative activities of ethyl acetate and n-butanol extracts of Centaurea sphaerocephalaL.

Abstract

This study aimed to evaluate the in vitro antiproliferative and inhibition of oxidative DNAdamage activities of n-butanol (n-BuOH) extract of Centaurea sphaerocephala. The in vitro antioxidant activity of the ethyl acetate (etOAc) and the n-BuOH extracts of this plant were also assayed. To investigate the antioxidant potential, extracts were tested for their capacity to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH · ) and to inhibit lipid peroxidation using the TBARs method. The contents of total phenolics and flavonoids were measured. Additionally, antiproliferative activity and DNA-damage inhibition of the n-BuOH extract was determined using XCelligence RTCA instrument and photolyzing 46966 plasmid, respectively. The results exhibited that the scavenging abilities of the etOAc extract were better than the n-BuOH extract with an IC 50 = 11.59 µg/ml and 16.67 µg/ml for both extracts, respectively. The phenolic and flavonoid contents were found higher in the n-BuOH and etOAc extracts. Furthermore, our results showed that n-BuOH extract exhibited a remarkable inhibition of lipid peroxidation with an IC 50 of 340.94±7.49 μg/mL and had an antiproliferative effect against Hela cells. Extracts of C. sphaerocephala showed antioxidant activity on scavenging DPPH · . In addition, the n-BuOH extract inhibited the lipid peroxidation and exhibited an antiproliferative effect against HeLa cells line (human cervix carcinoma).

Spectrophotometric Determination of Cobalt(II) and Lead(II) Using (1,5-Dimethyl-2-Phenyl-4-((2,3,4-Trihydroxy Phenyl) Diazenyl)-1H-Pyrazol-3(2H)-One) as Organic Reagent: Using It as Antimicrobial and Antioxidants

“…Therefore, the single band appeared at sites 1 and 3, which indicated the plasmid was not affected by the oxidizing agent due to the presence of compound (1,5-dimethyl-2-phenyl-4-((2,3,4-trihydroxy phenyl) diazenyl)-1H-pyrazol-3(2H)-one) working as antioxidants. These results are consistent with many studies such as those conducted by Razack et al [13] and by Lahneche et al [14].…”

Wild and Cultivated Centaurea raphanina subsp. mixta: A Valuable Source of Bioactive Compounds

“…Therefore, it seems that although antiproliferative activities of Centaurea sp. against tumor and non-tumor cell lines depend on the species and the tested plant tissue [93,102,106], the extraction method and the used solvents are also important indicating that the detected activities are associated with specific compounds with different polarities and solvent-depended extraction efficiency [99,100,107]. To the best of our knowledge, this is the first report regarding the cytotoxic effects of C. raphanina spp.…”

“…Similarly, Lockowandt et al [42] reported a significant correlation of phenolic acids content in C. cyanus plant parts (edible flowers and non-edible parts) with antioxidant activity, although this class of phenolic compounds was not detected in our study. In the study of Lahneche et al [99] n-butanol extracts of C. sphaerocephala which were richer in total phenolics and flavonoids exhibited higher antioxidant activity than other extracts. Moreover, several other reports tested the antioxidant activity of Centaurea species through various assays highlighting the potential role of different phytochemicals in such activities and the possible use of these species in human diet as antioxidant agents [86,[100][101][102][103][104].…”

Chemical Composition and Plant Growth of Centaurea raphanina subsp. mixta Plants Cultivated under Saline Conditions

“…Moreover, in the study of Lockowandt et al [29] where the cytotoxic effects of C. cyanus were evaluated, the authors did not report any toxic effects. However, several pharmacological studies have suggested that the cytotoxic effects and the biological activities of various Centaurea species are associated with their content in sesquiterpene lactones and flavonoids [25,31,98,99], although the solvents used for the acquisition of extracts may affect the phytochemicals composition in the extracts and therefore their in vitro cytotoxicity [32,100]. Therefore, any cultivation practices and processing of samples that may regulate the phytochemicals content of plants could also affect the bioactive properties of the obtained extracts.…”