Morphological and morphometric variations of Dicrocoelium rileyi (Digenea: Dicrocoelidae) parasitizing Tadarida brasiliensis (Chiroptera: Molosiidae) in Mexico

Abstract

Fifty-five adult Dicrocoelium rileyi Macy, 1931 parasitizing Tadarida brasiliensis (Geoffroy, 1824) from Durango, Nuevo Leon, Puebla, Zacatecas and Mexico State were morphologically described and morphometrically analyzed. To evaluate the degree of variation among populations from the five localities, 27 morphometric measures of this species were transformed into an orthogonal factor by principal component analysis (PCA), and a posterior comparison among populations was performed using discriminant analysis of principal components (DAPC). Significant differences were observed with exceptions between the Puebla -Zacatecas and Nuevo Leon -Mexico State populations when forming three groups with an 88 % assignation. The Durango population was the most dissimilar population. These results show that the morphological traits of D. rileyi are variable among the populations in this study due to local intraspecific variation; however, some of the specimens present in the distinct localities may represent different species. Molecular analysis is necessary to accurately define whether the populations involved in our study constitute one or several species.

Description of new species of Trematoda from bats of Southeastern Mexico and a new classification for Brachylecithum rileyi n. comb. (Dicrocoeliidae)

“…is a species of Dicrocoeliidae that was originally described as Dicrocoelium rileyi by Macy ( 1931 ) who found it parasitizing the intestine of Tadarida brasiliensis (Le Conte) in Kansas, USA. Since then, it has been recorded in several places in Mexico in the same species of bat (Caballero and Caballero 1969 ; Guzmán-Cornejo et al, 2003 ; Caspeta-Mandujano et al, 2017 ; Falcón-Ordaz et al, 2019 ) and in N . laticaudatus (Moguel-Chin et al, 2023 ).…”

“…Synonyms: Brachylecithum sp. in Moguel-Chin et al ( 2023 ), Dicrocoelium rileyi in Caballero and Caballero ( 1969 ); Caspeta-Mandujano et al ( 2017 ); Falcón-Ordaz et al ( 2019 ); Guzmán-Cornejo et al ( 2003 ); Martínez-Salazar et al ( 2020 ).…”

Validation of deep amplicon sequencing of Dicrocoelium in small ruminants from Northern regions of Pakistan

“…The flukes were fixed between two glass slides in formalin-acetic acid alcohol solution, stained with hematoxylin (Sigma-Aldrich) and mounted in Canada balsam. Each fluke was morphologically identified using standardised measurements, including the orientation of the testes, followed by twenty-six morphometric characters [ 9 , 23 , 24 ]. Eggs were isolated from the uterus of adult lancet flukes.…”

First record of Urotrema scabridum (Platyhelminthes), and new records of helminths of Tadarida brasiliensis from Mexican Plateau

“…Although the Mexican free-tailed bat is a widely distributed species, its helminth fauna has been seldom studied in Mexico (reported in all cases as T. brasiliensis); studies have been carried out in Mexico City and the states of Mexico, Durango, Morelos, Nuevo Leon, Puebla, and Zacatecas (Tuttle 1994, 1942, 1943Caballero y Caballero and Caballero-Rodríguez 1969;Guzmán-Cornejo et al 2003;Falcón-Ordaz et al 2006;García-Prieto et al 2012;Caspeta-Mandujano et al 2017;Jiménez et al 2017;Falcón-Ordaz et al 2019; Table 1). Sixteen helminth taxa have been recorded parasitizing this bat species in Mexico (Table 1): nine trematodes, two cestodes, four nematodes, and two nematodes.…”

“…Cordero et al 2014). To the best of our knowledge, only 28 bats species in the families Emballonuridae (one species), Molossidae (two species), Mormoopidae (four species), Natalidae (one species), Phyllostomidae (15 species) and Vespertilionidae (five species) have been examined for helminths (seeGarcía-Prieto et al 2012;Clarke- Crespo et al 2017;Caspeta-Mandujano et al 2017; Jiménez et al 2017; Salinas-Ramos et al 2017;Luviano-Hernández et al 2018;Falcón-Ordaz et al 2019), only 19.44 % of the bat species occurring in Mexico. Mexican insectivorous bats are one of the groups bearing the highest diversity of parasitic helminths.…”