Molecular epidemiology of 16S rRNA methyltransferase in Brazil: RmtG in Klebsiella aerogenes ST93 (CC4)

Passarelli‐Araujo, Hemanoel; Palmeiro, Jussara Kasuko; Moharana, Kanhu C.; Pedrosa-Silva, Francisnei; Dalla-Costa, Líbera Maria; Venâncio, Thiago M.

doi:10.1590/0001-376520182018762

Cited by 12 publications

(10 citation statements)

References 43 publications

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“…At present, the rmtG gene was still mainly prevalent in South America, especially in K. pneumoniae isolated from Brazil. It also could be found in K. aerogenes, P. aeruginosa, and E. coli isolated in America, India, Switzerland [ 3 , 59 , 96 , 97 , 98 , 100 , 101 ]. However, no strain carrying rmtG has been found in China so far.…”

Section: Plasmid-mediated 16s Rrna Methylase Resistance Gene and Its ...mentioning

confidence: 99%

Research Updates of Plasmid-Mediated Aminoglycoside Resistance 16S rRNA Methyltransferase

Yang

2022

Antibiotics

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With the wide spread of multidrug-resistant bacteria, a variety of aminoglycosides have been used in clinical practice as one of the effective options for antimicrobial combinations. However, in recent years, the emergence of high-level resistance against pan-aminoglycosides has worsened the status of antimicrobial resistance, so the production of 16S rRNA methyltransferase (16S-RMTase) should not be ignored as one of the most important resistance mechanisms. What is more, on account of transferable plasmids, the horizontal transfer of resistance genes between pathogens becomes easier and more widespread, which brings challenges to the treatment of infectious diseases and infection control of drug-resistant bacteria. In this review, we will make a presentation on the prevalence and genetic environment of 16S-RMTase encoding genes that lead to high-level resistance to aminoglycosides.

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Section: Plasmid-mediated 16s Rrna Methylase Resistance Gene and Its ...mentioning

confidence: 99%

Research Updates of Plasmid-Mediated Aminoglycoside Resistance 16S rRNA Methyltransferase

Yang

2022

Antibiotics

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“…The results also indicate that 11 ST93 isolates and 9 ST56 were clustered into one separate sub-cluster, respectively, which exhibited a slight difference in the core genome sequence. Recent studies found that ST93 was the most prevalent clone in the global K. aerogenes genome database, which indicated that ST93 might be dominant sequence type global clones in clinical settings [13,39]. Furthermore, the emergence of fecal carriage and human infection MDR K. aerogenes isolates resistant to multiple antibiotics, especially resistant to carbapenems is considered a substantial threat to public health [17,40].…”

Section: Whole Genome Sequencing and Comparison Analysismentioning

confidence: 99%

Comparative Genomic Analysis and Phenotypic Characterization of Bronchoscope-Associated Klebsiella Aerogenes

Huang

Chi

et al. 2020

Preprint

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Background: Bronchoscopes has been linked to the outbreaks of nosocomial infections. We aim to investigate the phenotypic and genomic profiles of bronchoscope-associated Klebsiella aerogenes isolates, and their association with genome public available isolates from human and environment.Methods: We performed a prospective single-center study sampling echoendoscopes after clinical use and after normal decontamination procedures. Bacterial screening was conducted by culturing the sample on Mueller-Hinton agar plates. Antimicrobial susceptibility testing was performed using the broth microdilution method. Whole-genome sequencing of K. aerogenes isolates was performed using an Illumina HiSeq system and comparative genomics analysis were conducted.Results: Over the 5-month period, a total of 358 isolates and 13 isolates were recovered from samples after clinical procedures and samples after decontamination procedures, respectively. Antimicrobial susceptibility testing found 7 K. aerogenes isolates to exhibit low-level resistance to antimicrobial agents. Among 7 K. aerogenes isolates, we found 5 sequence types (STs). Whole genome sequencing and comparison analysis observed the genetic diversity in our bacterial collection, which clustered into three main clades. Furthermore, we identified a total of 43 antimicrobial resistance genes in the K. aerogenes core genomes. As expected, human isolates encoded more antimicrobial resistance genes than that environmental isolates. Conclusions: This study first described the phenotypic and genomics characteristics of bronchoscope-associated K. aerogenes. The present observations demonstrated that broadly investigation of specific pathogens using publicly available global genomes offered the opportunity to identify prevalent clones associated with various hosts, sources, and geographical locations.

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“…Antibiotics have proved to be highly effective in treating a number of human infections and a preventative method for a variety of clinical treatments [12]. Antibiotic resistance is accelerated by the extensive and frequently ineffective use of antibiotics [13,14]. K. aerogenes isolates that harbor an extended range of beta-lactamase and carbapenemase genes and such strains are frequently associated with hospital outbreaks [15].…”

Section: Introductionmentioning

confidence: 99%

Development of a Candidate Multi-Epitope Subunit Vaccine against Klebsiella aerogenes: Subtractive Proteomics and Immuno-Informatics Approach

et al. 2021

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Klebsiella aerogenes is a Gram-negative bacterium which has gained considerable importance in recent years. It is involved in 10% of nosocomial and community-acquired urinary tract infections and 12% of hospital-acquired pneumonia. This organism has an intrinsic ability to produce inducible chromosomal AmpC beta-lactamases, which confer high resistance. The drug resistance in K. aerogenes has been reported in China, Israel, Poland, Italy and the United States, with a high mortality rate (~50%). This study aims to combine immunological approaches with molecular docking approaches for three highly antigenic proteins to design vaccines against K. aerogenes. The synthesis of the B-cell, T-cell (CTL and HTL) and IFN-γ epitopes of the targeted proteins was performed and most conserved epitopes were chosen for future research studies. The vaccine was predicted by connecting the respective epitopes, i.e., B cells, CTL and HTL with KK, AAY and GPGPG linkers and all these were connected with N-terminal adjuvants with EAAAK linker. The humoral response of the constructed vaccine was measured through IFN-γ and B-cell epitopes. Before being used as vaccine candidate, all identified B-cell, HTL and CTL epitopes were tested for antigenicity, allergenicity and toxicity to check the safety profiles of our vaccine. To find out the compatibility of constructed vaccine with receptors, MHC-I, followed by MHC-II and TLR4 receptors, was docked with the vaccine. Lastly, in order to precisely certify the proper expression and integrity of our construct, in silico cloning was carried out. Further studies are needed to confirm the safety features and immunogenicity of the vaccine.

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Molecular epidemiology of 16S rRNA methyltransferase in Brazil: RmtG in Klebsiella aerogenes ST93 (CC4)

Cited by 12 publications

References 43 publications

Research Updates of Plasmid-Mediated Aminoglycoside Resistance 16S rRNA Methyltransferase

Research Updates of Plasmid-Mediated Aminoglycoside Resistance 16S rRNA Methyltransferase

Comparative Genomic Analysis and Phenotypic Characterization of Bronchoscope-Associated Klebsiella Aerogenes

Development of a Candidate Multi-Epitope Subunit Vaccine against Klebsiella aerogenes: Subtractive Proteomics and Immuno-Informatics Approach

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