Molecular marker assisted selection for increasing inbreeding in S1 populations of cassava

Oliveira, Paulo Henrique G.A. De; Barbosa, Ana Claudia Oliveira; Diniz, Rafael Parreira; Oliveira, Eder Jorge de; Ferreira, Cláudia Fortes

doi:10.1590/0001-3765201820180278

Cited by 7 publications

(4 citation statements)

References 48 publications

(40 reference statements)

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“…This typically occurs due to gene products from the locus that prevent germination and/or pollen tube growth whenever the male (pollen) and female (pistil) tissues express the cognate S-alleles. This mechanism has evolved to prevent the occurrence of inbreeding in angiosperms, which often results in the considerable loss of agronomic performance [ 3 , 4 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, breeders can use sexual reproduction to produce segregating populations of new genotypes for breeding. In conventional breeding, seeds are produced through self-pollination or controlled crossings and then used as a source of genetic variation for the selection of superior genotypes and development of partially inbred lines [ 2 – 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Reproductive barriers in cassava: Factors and implications for genetic improvement

et al. 2021

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Cassava breeding is hampered by high flower abortion rates that prevent efficient recombination among promising clones. To better understand the factors causing flower abortion and propose strategies to overcome them, we 1) analyzed the reproductive barriers to intraspecific crossing, 2) evaluated pollen-pistil interactions to maximize hand pollination efficiency, and 3) identified the population structure of elite parental clones. From 2016 to 2018, the abortion and fertilization rates of 5,748 hand crossings involving 91 parents and 157 progenies were estimated. We used 16,300 single nucleotide polymorphism markers to study the parents’ population structure via discriminant analysis of principal components, and three clusters were identified. To test for male and female effects, we used a mixed model in which the environment (month and year) was fixed, while female and male (nested to female) were random effects. Regardless of the population structure, significant parental effects were identified for abortion and fertilization rates, suggesting the existence of reproductive barriers among certain cassava clones. Matching ability between cassava parents was significant for pollen grains that adhered to the stigma surface, germinated pollen grains, and the number of fertilized ovules. Non-additive genetic effects were important to the inheritance of these traits. Pollen viability and pollen-pistil interactions in cross- and self-pollination were also investigated to characterize pollen-stigma compatibility. Various events related to pollen tube growth dynamics indicated fertilization abnormalities. These abnormalities included the reticulated deposition of callose in the pollen tube, pollen tube growth cessation in a specific region of the stylet, and low pollen grain germination rate. Generally, pollen viability and stigma receptivity varied depending on the clone and flowering stage and were lost during flowering. This study provides novel insights into cassava reproduction that can assist in practical crossing and maximize the recombination of contrasting clones.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reproductive barriers in cassava: Factors and implications for genetic improvement

et al. 2021

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“…Individuals with higher levels of attained homozygosity would be prioritized to carry out the subsequent generation of self-pollination, fast-tracking the production of inbred lines when compared to simple random selection of offsprings. Despite this obvious application of DNA marker data to accelerate the buildup of inbreeding in outbred plants, reports to this end have been rare, using only low-resolution microsatellite genotyping in papaya [ 20 ] and cassava [ 21 ]. In both studies, plants with higher-than-expected homozygosity could be identified already in the initial generations of self-pollination.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide SNP genotyping as a simple and practical tool to accelerate the development of inbred lines in outbred tree species: An example in cacao (Theobroma cacao L.)

Lopes

Pires²,

Gramacho³

et al. 2022

PLoS ONE

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Cacao is a globally important crop with a long history of domestication and selective breeding. Despite the increased use of elite clones by cacao farmers, worldwide plantations are established mainly using hybrid progeny material derived from heterozygous parents, therefore displaying high tree-to-tree variability. The deliberate development of hybrids from advanced inbred lines produced by successive generations of self-pollination has not yet been fully considered in cacao breeding. This is largely due to the self-incompatibility of the species, the long generation cycles (3–5 years) and the extensive trial areas needed to accomplish the endeavor. We propose a simple and accessible approach to develop inbred lines based on accelerating the buildup of homozygosity based on regular selfing assisted by genome-wide SNP genotyping. In this study we genotyped 90 clones from the Brazilian CEPEC´s germplasm collection and 49 inbred offspring of six S1 or S2 cacao families derived from self-pollinating clones CCN-51, PS-13.19, TSH-1188 and SIAL-169. A set of 3,380 SNPs distributed across the cacao genome were interrogated on the EMBRAPA multi-species 65k Infinium chip. The 90 cacao clones showed considerable variation in genome-wide SNP homozygosity (mean 0.727± 0.182) and 19 of them with homozygosity ≥90%. By assessing the increase in homozygosity across two generations of self-pollinations, SNP data revealed the wide variability in homozygosity within and between S1 and S2 families. Even in small families (<10 sibs), individuals were identified with up to ~1.5 standard deviations above the family mean homozygosity. From baseline homozygosities of 0.476 and 0.454, offspring with homozygosities of 0.862 and 0.879 were recovered for clones TSH-1188 and CCN-51 respectively, in only two generations of selfing (81–93% increase). SNP marker assisted monitoring and selection of inbred individuals can be a practical tool to optimize and accelerate the development of inbred lines of outbred tree species. This approach will allow a faster and more accurate exploitation of hybrid breeding strategies in cacao improvement programs and potentially in other perennial fruit and forest trees.

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“…Phenotypic recurrent selection is one of the main breeding methods used for selecting segregating populations derived from crosses between highly elite genotypes selection (Ceballos et al,2012;De Oliveira et al,2018). This method has been used in several maize breeding programmes to improve resistance or tolerance of maize to abiotic or biotic stress (Kamara et al,2003, Menkir andKling, 2007;Badu-Apraku et al,2012).…”

Section: Introductionmentioning

confidence: 99%

Agronomic Performance Of S1 Maize Lines Derived From A Bi-Parental Cross Under Infested And Striga Free Environments

Yacoubou

Wallis

Salami

et al. 2021

ESJ

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Striga hermonthica, causes up to 100% yield loss in maize production in sub-Saharan Africa. Developing Striga resistant maize cultivars could be a major component of integrated Striga management strategies. This study aims at assessing the agronomic performance of S1 breeding lines in improving maize for Striga resistance. Two hundred S1 lines have been evaluated under artificial infestation Striga and Striga-free conditions in Benin for two years during 2018 and 2019 growing seasons using alpha-lattice design (51 x 4) with two replicates. Twelve agro-morphological and Striga adaptive traits have been assessed. The tested lines have displayed high genetic variability for most agronomic and Striga adaptive traits. The S1 lines exhibited high grain yield than their parents with averages of 2,552.72±593 kg ha-1 and 2,965.67±635.86 kg ha-1 under Striga artificial infestation and Striga-free conditions, respectively. Grain yield has displayed high positive and significant genetic and phenotypic correlations with ears per plant and high negative correlations with days to 50% silking, ears aspect, and Striga damage rating at 8 and 10 weeks after planting (WAP). Useful traits like ears per plant, days to 50% silking, ears aspect, number of emerged Striga plants and Striga rating at 10 WAP could assist for indirect selection under Striga conditions. Based on the selection index, a total of 15 S1 lines have been identified as top ranking and can be used as sources of resistance or tolerance genes to Striga and further improvement in maize breeding in future.

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Molecular marker assisted selection for increasing inbreeding in S1 populations of cassava

Cited by 7 publications

References 48 publications

Reproductive barriers in cassava: Factors and implications for genetic improvement

Reproductive barriers in cassava: Factors and implications for genetic improvement

Genome-wide SNP genotyping as a simple and practical tool to accelerate the development of inbred lines in outbred tree species: An example in cacao (Theobroma cacao L.)

Agronomic Performance Of S1 Maize Lines Derived From A Bi-Parental Cross Under Infested And Striga Free Environments

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