ABA regulation of post-germination desiccation tolerance in wheat cultivars contrasting in drought tolerance

Kour, Satinder; Zhawar, Vikramjit Kaur

doi:10.1590/0001-3765201820170632

Cited by 3 publications

(5 citation statements)

References 18 publications

(14 reference statements)

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“…As this was observed higher in drought tolerant cultivar PBW 644 hence post-germination DT could be a good marker for vegetative drought tolerance of the plant. This visual growth data has also been confirmed in dry biomass measurement which is given in detail in our other report [20]. Removal of ROS/NO under CT and ABA had reduced growth of rehydrated seedlings of almost both cultivars, hence indicated the significant contribution of ROS/NO-pathways in post-germination DT.…”

Section: Discussionsupporting

confidence: 78%

“…We also found increase of ascorbate during desiccation in control seedlings but ABA/PEG supply decreased this content. These supplies also caused more water-loss and growth arrest [20]. Therefore, ABA/PEG could decrease ascorbate for improving desiccation tolerance as ascorbate besides acting as antioxidant, stimulates cell division and cell expansion [15].…”

Section: Discussionmentioning

confidence: 99%

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ABA regulation of antioxidant activity during post-germination desiccation and subsequent rehydration in wheat

Kour

Zhawar

2018

Acta Biologica Hungarica

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ABA regulation of antioxidant activity during post-germination desiccation and subsequent rehydration was studied in two wheat cultivars PBW 644 (ABA-higher sensitive and drought tolerant) and PBW 343 (ABA-lesser sensitive and drought susceptible) where 1 d-germinated seeds were exposed to ABA/ PEG-6000 for next 1 d, desiccated for 4 d and subsequently rehydrated for 4 d. Ascorbate, dehydrascorbate to ascorbate ratio, malondialdehyde (MDA), hydroxyl radicals, and activities of monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), alcohol dehydrogenase (AlcDH) and aldehyde dehydrogenase (AldDH) were measured in seedlings just before desiccation (2 d old), desiccated (6 d old) and rehydrated (10 d old) stages. ROS/NO signaling was studied under CT and ABA supply by supplying ROS and NO scavengers. During desiccation, both cultivars showed increase of oxidative stress (dehydroascorbate to ascorbate ratio, MDA, hydroxyl radicals) and antioxidant activity in the form of ascorbate content and AldDH activity while other antioxidant enzymes were not increased. PBW 644 showed higher antioxidant activity thus produced less oxidative stress compared to PBW 343. During rehydration, activities of all antioxidant enzymes and levels of ROS (hydroxyl radicals) were increased in both cultivars and MDA was decreased in PBW 343. ABA supply improved desiccation as well as rehydration by improving all parameters of antioxidant activity tested in this study. PEG supply resembled to ABA-supply for its effects. ABA/PEG improvements were seen higher in PBW 644. ROS/ NO-signalling was involved under CT as well as under ABA for increasing antioxidant activity during desiccation as well as rehydration in both cultivars.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

ABA regulation of antioxidant activity during post-germination desiccation and subsequent rehydration in wheat

Kour

Zhawar

2018

Acta Biologica Hungarica

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“…Gene ST55G715 encodes NCED and was significantly induced under drought, confirming that drought conditions enhance the synthesis of ABA. The rapid accumulation of ABA promotes stomatal closure, thereby reducing the water loss caused by water transpiration [60]. In addition, ABA further inhibits the activity of phosphatase 2C (PP2C) after binding to its receptor, PYR/PYL/RCAR, to release SnRK2's autophosphorylation and protein phosphorylation activity, which ultimately activates downstream ABA-dependent TFs, such as MYB factors [61].…”

Section: Discussionmentioning

confidence: 99%

Identifying resurrection genes through the differentially expressed genes between Selaginella tamariscina (Beauv.) spring and Selaginella moellendorffii Hieron under drought stress

Zhang

Sun

et al. 2019

PLoS ONE

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Selaginella tamariscina (Beauv.) spring, a primitive vascular resurrection plant, can survive extreme drought and recover when water becomes available. To identify drought-inducible genes and to clarify the molecular mechanism of drought tolerance, a comparative transcriptional pattern analysis was conducted between S. tamariscina and Selaginella moellendorffii Hieron (drought sensitive). 133 drought related genes were identified, including 72 functional genes and 61 regulatory genes. And several drought responsive reactions, such as antioxidant activity, osmotic balance, cuticle defense and signal transduction were highlighted in S. tamariscina under drought. Notably, besides peroxidase, catalase and L-ascorbate oxidase genes, DEGs associated with phenylalanine metabolism and polyamine catabolism could be alternative ways to enhance antioxidant ability in S. tamariscina. DEGs related to soluble carbohydrate metabolism, late embryogenesis abundant protein (LEA) and aquaporin protein (AQP) confirmed that osmotic adjustment could resist drought during desiccation. DEGs involved in xyloglucan metabolic process, pectin metabolic process and cutin biosynthesis may also contribute to drought tolerance of S. tamariscina by cuticle defense. Drought-responsive genes encoding protein kinases, calcium sensors, transcription factors (TFs) and plant hormones also help to drought resistance of S. tamariscina. The preliminary validation experiments were performed and the results were consistent with our hypothetical integrated regulatory network. The results of this study provide candidate resurrection genes and an integrated regulatory network for further studies on the molecular mechanisms of stress tolerance in S. tamariscina.

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“…In this case, it was likely to scavenge the drought‐induced ROS accumulation and therefore protect plant cells from oxidative damage. Existing studies suggest that the application of exogenous ABA could increase the activities of SOD, CAT and POD, and thus enhance the ability of scavenging free radicals in wheat under water stress 53,54 . ABA also can act as an important regulator to induce drought‐induced antioxidant defense response and prevent oxidative damage in maize and rice amongst other crops 55 .…”

Section: Discussionmentioning

confidence: 99%

“…Existing studies suggest that the application of exogenous ABA could increase the activities of SOD, CAT and POD, and thus enhance the ability of scavenging free radicals in wheat under water stress. 53,54 ABA also can act as an important regulator to induce drought-induced antioxidant defense response and prevent oxidative damage in maize and rice amongst other crops. 55 Our observations confirmed that ABA, as one of the important signal substances, can stimulate the production of nHRS and regulate the balance of ROS accumulation, antioxidant enzyme activities and oxidative damage, which contributed to enhancing drought adaptability in grass pea.…”

Section: Discussionmentioning

confidence: 99%

Role of abscisic acid in modulating drought acclimation, agronomic characteristics and β‐N‐oxalyl‐L‐α,β‐diaminopropionic acid (β‐ODAP) accumulation in grass pea (Lathyrus sativusL.)

et al. 2021

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BACKGROUND: ⊎-N-oxalyl-L-⊍,⊎-diaminopropionic acid (⊎-ODAP) is a physiological indicator in response to drying soil. However, how abscisic acid (ABA) modulates ⊎-ODAP accumulation and its related agronomic characteristics in drought stressed grass pea (Lathyrus sativus L.) continue to be unclear. The present study aimed to evaluate the effects of ABA addition on drought tolerance, agronomic characteristics and ⊎-ODAP content in grass pea under drought stress.RESULTS: Exogenous ABA significantly promoted ABA levels by 19.3% and 18.3% under moderate and severe drought stress, respectively, compared to CK (without ABA, used as control check treatment). ABA addition activated earlier trigger of nonhydraulic root-sourced signal at 69.1% field capacity (FC) (65.5% FC in CK) and accordingly prolonged its operation period to 45.6% FC (49.0% FC in CK). This phenomenon was mechanically associated with the physiological mediation of ABA, where its addition significantly promoted the activities of leaf superoxide dismutase, catalase and peroxidase enzymes and the biosynthesis of leaf proline, simultaneously lowering the accumulation of malondialdehyde and hydrogen peroxide under moderate and severe stresses. Interestingly, ABA application significantly increased seed ⊎-ODAP content by 21.7% and 21.3% under moderate and severe drought stress, but did not change leaf ⊎-ODAP content. Furthermore, ABA application produced similar shoot biomass and grain yield as control groups.CONCLUSION: Exogenous ABA improved the drought adaptability of grass pea and promoted the synthesis of ⊎-ODAP in seeds but not in leaves. Our findings provide novel insights into the agronomic role of ABA in relation to ⊎-ODAP enrichment in grass pea subjected to drought stress.

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ABA regulation of post-germination desiccation tolerance in wheat cultivars contrasting in drought tolerance

Cited by 3 publications

References 18 publications

ABA regulation of antioxidant activity during post-germination desiccation and subsequent rehydration in wheat

ABA regulation of antioxidant activity during post-germination desiccation and subsequent rehydration in wheat

Identifying resurrection genes through the differentially expressed genes between Selaginella tamariscina (Beauv.) spring and Selaginella moellendorffii Hieron under drought stress

Role of abscisic acid in modulating drought acclimation, agronomic characteristics and β‐N‐oxalyl‐L‐α,β‐diaminopropionic acid (β‐ODAP) accumulation in grass pea (Lathyrus sativusL.)

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