Decolorization of remazol brilliant blue R with laccase from Lentinus crinitus grown in agro-industrial by-products

Almeida, P. H. de; Oliveira, Ana Carolina Costa de; Souza, Genyfer Poline Neri de; Friedrich, Juliana Cristhina; Linde, Giani Andréa; Colauto, Nelson Barros; Valle, Juliana Silveira do

doi:10.1590/0001-3765201820170458

Cited by 24 publications

(18 citation statements)

References 43 publications

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“…We found that L. crinitus did not produce MnP in SSC with sugarcane bagasse. These results are in agreement with Almeida et al [28] that cultivated the same strain in liquid medium added with coffee husks or citric pulp and reported laccase but not LiP and MnP production. When L. crinitus was cultivated in synthetic liquid medium with glucose or fructose added with various nitrogen sources [29], MnP and LiP activities were not detected also.…”

Section: Discussionsupporting

confidence: 93%

“…Polyphenols can induce the production of ligninolytic enzymes [39], and these compounds, solubilized during the cultivation period, could have induced the laccase production by L. crinitus. Almeida et al [28] cultivated the same strain of L. crinitus used in this study in liquid medium containing coffee husks or citric pulp, as the only carbon sources, added with urea (0.7 g/ L or 11.2 g/L nitrogen, respectively). Both agro-industrial byproducts contain polyphenols, and the authors suggested that the greater polyphenol proportion in coffee husks could have contributed for a greater laccase production with this byproduct of approximately 41,250 U/L and 156 U L −1 h −1 .…”

Section: Discussionmentioning

confidence: 99%

“…This mushroom is capable to produce great amounts of mycelial biomass with antioxidant activity [24] besides being known as a robust species that produces enzymes such as laccase [25], cellulase, and xylanase [26] under different cultivation conditions [27]. Its capacity to degrade dyes of different classes has already been demonstrated [28,29], but there are no data on the laccase production of this species by SSC. In this study, we evaluated the production of ligninolytic enzymes by L. crinitus in SSC with sugarcane bagasse and the enzymatic extract's capacity to decolorize azo and anthraquinone dyes.…”

Section: Introductionmentioning

confidence: 99%

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Decolorization of azo and anthraquinone dyes by crude laccase produced by Lentinus crinitus in solid state cultivation

et al. 2019

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White-rot basidiomycetes such as Lentinus crinitus produce laccases with potential use in dye biodegradation. However, high productivity and enzymes with specific properties are required in order to make viable laccase production. We aimed to produce laccase from Lentinus crinitus grown in sugarcane bagasse for dye decolorization. Solid state cultivation medium had sugarcane bagasse added with a nutrient solution of 10 g/L glucose, 1 g/L KH 2 PO 4 , 0.5 g/L MgSO 4 , 0.001 g/L FeSO 4 , 0.01 g/L ZnSO 4 , and 0.01 g/L MnSO 4. The addition of different nitrogen sources (peptone, urea, or peptone plus urea) and different nitrogen concentrations (0, 0.4, 0.6, 0.8, 1.0, and 1.2 g/L) were evaluated. Enzymatic extract was used in the decolorization of azo dyes, reactive blue 220 (RB220) and reactive black 5 (RB5), and anthraquinone dye, Remazol brilliant blue R (RBBR). The greatest laccase activity (4800 U/ g dry mass) occurred when the peptone and urea mixture was added to the solid state cultivation medium. When the nitrogen concentration was 1 g/L, the laccase activity increased to 6555 U/g dry mass. The laccase activity peak occurred on the 10th day, and the maximum decolorization within 24 h was observed with enzymatic extracts obtained on different cultivation days, i.e., 6th day for RB220, 10th day for RB5, and 9th day for RBBR. Manganese and lignin peroxidases were not produced when nitrogen was added to the cultivation medium. The crude enzymatic extract was more effective in the decolorization of azo dyes (RB220 and RB5), more than 90% of decolorization, than anthraquinone dye with 77% decolorization.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Decolorization of azo and anthraquinone dyes by crude laccase produced by Lentinus crinitus in solid state cultivation

et al. 2019

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“…The enzyme was more efficient in decolourizing RBBR with 45% and 93% after 1 hour and 24 hours respectively. However, another study reported 74.4% and 76% decolourization of RBBR for 6 and 12 days using laccase produced on Citric pulp and Coffe husks respectively by Lentinus crinitus [56]. Congo red, Methylene blue and Crystal violet successfully achieved 51%, 53% and 60% decolourization at 120, 48 and 72 hours respectively.…”

Section: Decolourization Of Synthetic Dyesmentioning

confidence: 96%

Production of Fungal Laccase under Solid State Bioprocessing of Agroindustrial Waste and Its Application in Decolourization of Synthetic Dyes

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Oluma

et al. 2019

JABB

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Fungal laccases are preferred due to high redox potentials and low substrate specificity to xenobiotics including synthetic dyes. For large-scale applications, low enzyme yield and high cost of production has remained the challenge. Agroindustrial waste such as saw-dust of Terminalia superba abounds locally and was utilized as low-cost alternative substrate for laccase production in Solid State Bioprocessing (SSB) using Trametes sp. isolate G31. The study optimized laccase production using various parameters. Optimal production of laccase was at pH 5.0 - 7.0 with 2356 U/mL - 2369 U/mL and 25°C (2336 U/mL). Among the sources of nitrogen and carbon tested, laccase production in ammonium sulphate and sucrose supplemented media were higher. The effect of activators on laccases production showed that Cu2+ and Ca2+induced high titres of laccase at 4 -5 mM and 2 - 4 mM respectively, while production of laccase by Mn2+ was significantly high at 40 mM. The effect of 2, 2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), guaiacol and varatryl alcohol on laccase production was significantly different especially in glycerol. Optimum production for laccase was on day 14 with 2356 U/mL followed by steady declined up to day 34. The purified laccase had a specific activity of 5008 µmol/min/mg, purification factor of 3.85, and a molecular mass of ~40 kDa using N-PAGE. The potential of crude laccase to decolourize diverse dyes was tested. Phenol red achieved 40% decolourization for 1hr, while RBBR (93%), Crystal violet (60%), Methylene blue (53%) and Congo red (51%) for 24 hr, 72 hr, 48 hr and 120 hr respectively. Methyl red and Malachite green attained 42% (72 hr) and 32% (48 hr) decolourization. The enzyme ability to oxidize Phenol red and other synthetic dyes without mediators made it eco-friendly in treating dye wastewaters.

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“…As mentioned before, blue light significantly reduced mycelium biomass growth of L. crinitus in liquid cultivation. This species is a robust fungus that can grow over a wide range of substrates (ALMEIDA et al, 2018), temperatures and pH of the cultivation media (MARIM et al, 2018), however, blue light negatively influenced the biomass yield. Our results are similar to the one reported by Nakano et al (2010) who cultivated Pleurotus ostreatus in Petri dishes under blue light with suppression of mycelial biomass growth according to light intensity.…”

Section: Enzymatic Activity or Mycelial Biomassmentioning

confidence: 99%

Lentinus crinitus response to blue light on carbohydrate-active enzymes

et al. 2020

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Fungi are capable of sensing light from ultraviolet to far-red and they use light as a source of information about the environment anticipating stress and adverse conditions. Lentinus crinitus is a lignin-degrading fungus which produces laccase and other enzymes of biotechnological interest. The effect of blue light on fungal enzymatic activity has been studied; however, it has not been found studies on the effect of the blue light on carbohydrate-active enzymes and on mycelial biomass production of L. crinitus. We aimed to investigate carbohydrate-active enzymes activity and mycelial biomass production of L. crinitus cultivated under continuous illumination with blue light. L. crinitus was cultivated in malt extract medium in the dark, without agitation, and under continuous illumination with blue light-emitting diodes. The blue light reduced the total cellulase, pectinase and xylanase activities but increased the endoglucanase activity. Blue light reduced the mycelial growth of L. crinitus in 26% and the enzymatic activity-to-mycelial biomass ratio (U mg-1 dry basis) increased in 10% total cellulase, 33% endoglucanase, and 16% pectinase activities. Also, it is suggested that L. crinitus has a photosensory system and it could lead to new process of obtaining enzymes of biotechnological interest.

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Decolorization of remazol brilliant blue R with laccase from Lentinus crinitus grown in agro-industrial by-products

Cited by 24 publications

References 43 publications

Decolorization of azo and anthraquinone dyes by crude laccase produced by Lentinus crinitus in solid state cultivation

Decolorization of azo and anthraquinone dyes by crude laccase produced by Lentinus crinitus in solid state cultivation

Production of Fungal Laccase under Solid State Bioprocessing of Agroindustrial Waste and Its Application in Decolourization of Synthetic Dyes

Lentinus crinitus response to blue light on carbohydrate-active enzymes

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