Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus

Abstract

This study aimed to determine the histological features of the endometrium of bitches, as well as the cell proliferation at specific moments of diestrus, 10, 20, 30, 40, 50 and 60 days post ovulation, correlating the endometrial thickness with the uterine cell proliferation and the metabolic state (weight, blood glucose and plasma cholesterol) of the animals. Therefore, the right and left uterine horns of 26 clinically healthy bitches submitted to ovariohysterectomy were histologically analyzed 10, 20, 30, 40, 50 and 60 days post ovulation. The hematoxylin-eosin and AgNOR staining techniques were performed. All parameters were evaluated by ANOVA and post-hoc Tukey test (p<0.05). The correlation between endometrial thickness and uterine cell proliferation, weight, blood glucose and plasma cholesterol of animals was observed using the Pearson method (p<0.05). In the present study, it is concluded that endometrial thickness does not differ at any of the moments analyzed in diestrus. The endometrial thickness is not influenced by hormones, weight, blood glucose or serum cholesterol of bitches in this phase of the estrous cycle. However, there is greater cell proliferation in the endometrium at day 40 compared to day 60 post ovulation under the influence of the endocrine profile.

Peroxisome proliferator‐activated receptor expression in the canine endometrium with cystic endometrial hyperplasia–pyometra complex

“…The results of our present study were conducted at diestrus and revealed serum P 4 concentrations (ng/ml) as follows (mean ± SD ): 35.97 ± 2.81 in the control group, 33.63 ± 3.16 in the CEH and 29.93 ± 2.22 in the CEH‐P groups, which is consistent with the results of P 4 measurements noted in previous reports (Concannon, 2011; Gossler et al., 2017; Hoffmann et al., 2004). Interestingly, despite the still high serum P 4 levels, in the CEH‐P group, it was downregulated compared to the control group and the CEH group.…”

“…The present study is the first ever report that demonstrates PPAR after ovulation fluctuate between 30 and 35 ng/ml and then gradually decline to <1 ng/ml (Concannon, 2011;Gossler et al, 2017;Hoffmann et al, 2004).…”

“…As previously described, E 2 presents variable concentrations and values ranging from 15 to 30 pg/ml, which are the highest in the middle of diestrus, after which they decreased (Concannon, 2011; Gossler et al., 2017; Papa & Hoffmann, 2011). Therefore, under the influence of P 4 and E 2 , the canine endometrium undergoes architectonical and biochemical alterations involved in proliferation, apoptosis and cellular differentiation (Bosschere et al, 2001).…”

“…At diestrus phase in the bitch there is an increase in serum P 4 concentrations in the first half of its duration and fluctuations in serum E 2 concentrations in the second half. It has been shown that P 4 concentrations in peripheral blood around Day 30 after ovulation fluctuate between 30 and 35 ng/ml and then gradually decline to <1 ng/ml (Concannon, 2011; Gossler et al., 2017; Hoffmann et al., 2004).…”

Vitrification of ovarian tissue of Brazilian North‐eastern donkeys (Equus asinus) using different cryoprotectants

“…The ribosomes, when linked to the RNA messenger, form polyribosomes, which are responsible for the synthesis of all proteins, including proteins that will form new cells (Brar & Weissman, ). For this reason, NORs are directly related to cellular proliferative activity, and the greater the proliferative activity of the cell, the greater the quantity of NORs observed (Gossler et al., ). After the identification and counting of NORs, the same amount of NORs were observed by cells from the control follicles and those vitrified in the combination of DMSO + EG.…”