Lipolytic response of adipose tissue and metabolic adaptations to long periods of fasting in red tilapia (Oreochromis sp., Teleostei: Cichlidae)

Júnior, Walter Dias; Baviera, Amanda Martins; Zanon, Neusa Maria; Galban, V D; Garófalo, Maria Antonieta Rissato; Machado, C. R.; Bailão, Elisa Flávia Luiz Cardoso; Kettelhut, Ísis C.

doi:10.1590/0001-3765201620150484

Cited by 14 publications

(6 citation statements)

References 36 publications

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“…The moment when liver glycogen reserves begin to decrease is variable but is thought to range from 5 to 20 days after food is removed (Sangiao-Alvarellos et al 2005;Barcellos et al 2010). Furné et al (2012) observed that, in rainbow trout, hepatic glycogen is mobilized after 5 days of fasting, which agrees with our results and reflects how glycogen is used to replace the absence of dietary carbohydrate intake (Dias Junior et al 2016). However, muscle glycogen did not decrease until 20 days of food deprivation (200 °C d), possibly because it makes a small contribution to total energy expenditure and can be maintained at the expense of hepatic glucose release (Navarro and Gutiérrez 1995).…”

Section: Liver/muscle Glycogen and Liver Colorsupporting

confidence: 87%

Physio-metabolic response of rainbow trout during prolonged food deprivation before slaughter

Bermejo‐Poza

Fernández-Muela

Fuente

et al. 2018

Fish Physiol Biochem

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Fish normally undergo periods of food deprivation that are longer than non-hibernating mammals. In aquacultured rainbow trout (Oncorhynchus mykiss), it is unclear how fasting may affect their physiological adaptative response, especially when they are normally fed daily. In addition, that response may vary with temperature, making it necessary to express fasting duration in terms of degree days. In the current study, trout were fasted for 5, 10, and 20 days (55, 107, and 200 degree days (°C d), respectively). To assess the physiological response of fish to fasting, different biometric, blood, plasma, and metabolic parameters were measured, as well as liver fatty acid composition. The fish weight, condition factor, and the hepato-somatic index of 5-day fasted trout were not significantly different from those of control fish. Gastric pH increased as fasting progressed while plasma concentrations of glucose, triglycerides, and total proteins decreased significantly after 10 days of fasting, while the percentage of non-esterified fatty acids increased. There were no significant differences in plasma ions (sodium, potassium, and calcium), except for chloride ion which decreased after 5 days of fasting. Liver glycogen decreased after 5 days of fasting while glycogen concentration in muscle did not decrease until 20 days of fasting. Liver color presented a higher chroma after 5 days of fasting, suggesting a mobilization of reserves. Finally, acetylcholinesterase activity in the brain was not affected by food deprivation but increased after 10 days of fasting in liver and muscle, suggesting the mobilization of body reserves, but without severely affecting basal metabolism.

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Section: Liver/muscle Glycogen and Liver Colorsupporting

confidence: 87%

Physio-metabolic response of rainbow trout during prolonged food deprivation before slaughter

Bermejo‐Poza

Fernández-Muela

Fuente

et al. 2018

Fish Physiol Biochem

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“…Fasting produces a decrease in liver glycogen levels when used to replace the absence of dietary carbohydrate intake (Dias Junior et al, ). It has been reported to decrease in rainbow trout after 5 day of fasting (Furné et al, ), which agrees with our results.…”

Section: Discussionmentioning

confidence: 99%

Fasting combined with long catch duration modifies the physio‐metabolic response and flesh quality of rainbow trout

et al. 2019

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Pre‐slaughter handling involves fasting fish and catching them, which can affect fish welfare and flesh quality, but few studies have considered their combined effects. In this study, adult rainbow trout (320 ± 10 g average weight) were fasted for 7 days (135.6 degree days) and subjected to a long catch duration (20 min), compared with controls (no fasting or short catch duration). Condition factor, organ weight indexes and carcass yield decreased with fasting but not catch duration. Plasma concentrations of cortisol, glucose and lactate increased after a long catch, while plasma triglycerides decreased with fasting. Liver glycogen concentration was lower in fasted fish, and liver luminosity and chroma were higher after fasting with a long catch. Regarding flesh quality, rigor mortis resolved more slowly and final muscle pH at 48 hr post‐mortem was higher for fasted fish with a long catch time. Muscle glycogen concentration was higher in fasted fish, where chroma was also lower. Fasted fish had lower lipid oxidation, but there were no differences in fat content in muscle. Fasted fish with a long catch duration also had less monounsaturated and more saturated fatty acids. In conclusion, a long catch triggered a stress response that had negative effects on flesh quality, independently of fasting.

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“…Hepatocytes were classified according to their accumulations as accentuated and diffuse glycogen accumulation (+); moderate and diffuse/multifocal glycogen accumulation (++); or mild glycogen accumulation concomitant to multifocal or diffuse fatty accumulation (+++). The accumulation scores were based on the normal glycogenic accumulations in healthy captive‐reared fish (Wolf et al, 2015) and on the presence of fatty accumulations after a starvation period in the tilapia (Dias‐Junior et al, 2016). The liver slides were also evaluated for the presence and intensity of granuloma and inflammatory infiltrate.…”

Section: Methodsmentioning

confidence: 99%

Administration of dehydrated oxytetracycline effectively reduces francisellosis mortality in Nile tilapia

Favero

Facimoto

Chideroli

et al. 2021

Aquaculture Research

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Francisellosis is a disease responsible for severe economic losses in aquaculture. Currently, this disease is controlled via biosecurity measures and treatment of affected batches. In this study, tilapia juveniles were challenged with Francisella orientalis via immersion and fed with oxytetracycline‐medicated feed, at 100 mg kg fish−1 and 200 mg kg fish−1, under prophylactic and therapeutic strategies for 16 and 15 days respectively. All doses and treatment strategies reduced the fish mortality. The detection of the pathogen from the spleen was lower in the treated groups. Histopathology analysis showed that challenged fish that received the highest dosage presented fewer granuloma in their spleens and less inflammatory infiltrate in their livers than untreated fish. Fish that received the prophylactic treatment, regardless of the dose, presented fewer granuloma in their livers than untreated fish. Fish treated with 100 mg kg fish−1 had better scores for hepatocyte accumulations than fish treated with the higher dosage. Furthermore, 20% of the unchallenged fish that received the higher dosage presented mild inflammatory infiltrate in their livers. No differences were observed in the serum hepatocyte lesion enzyme quantifications. The results indicate that oxytetracycline‐medicated feed is safe, even at the higher dose, and effective at controlling francisellosis in tilapia.

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Lipolytic response of adipose tissue and metabolic adaptations to long periods of fasting in red tilapia (Oreochromis sp., Teleostei: Cichlidae)

Cited by 14 publications

References 36 publications

Physio-metabolic response of rainbow trout during prolonged food deprivation before slaughter

Physio-metabolic response of rainbow trout during prolonged food deprivation before slaughter

Fasting combined with long catch duration modifies the physio‐metabolic response and flesh quality of rainbow trout

Administration of dehydrated oxytetracycline effectively reduces francisellosis mortality in Nile tilapia

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