From Gene Targeting to Genome Editing: Transgenic animals applications and beyond

Rocha-Martins, Maurício; Cavalheiro, Gabriel R.; Matos-Rodrigues, Gabriel; Martins, Rodrigo A. P.

doi:10.1590/0001-3765201520140710

Cited by 38 publications

(18 citation statements)

References 186 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Animal models have improved in the past few years, and these modified animals now present more accurate models to medical research. For example, scientists can modulate animal genomes by adding or deleting genes to mimic disease or to study the function of these genes [ 12 ]. These animals are called transgenic animals.…”

Section: Introductionmentioning

confidence: 99%

Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing

et al. 2020

View full text Add to dashboard Cite

Animal testing has long been used in science to study complex biological phenomena that cannot be investigated using two-dimensional cell cultures in plastic dishes. With time, it appeared that more differences could exist between animal models and even more when translated to human patients. Innovative models became essential to develop more accurate knowledge. Tissue engineering provides some of those models, but it mostly relies on the use of prefabricated scaffolds on which cells are seeded. The self-assembly protocol has recently produced organ-specific human-derived three-dimensional models without the need for exogenous material. This strategy will help to achieve the 3R principles.

show abstract

Section: Introductionmentioning

confidence: 99%

Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Sequence-specific DNA binding, such as the interaction between a transcription factor and a promoter, is a common phenomenon. For genome editing, the previously mentioned nucleases can target specific sequences to generate DSBs under the guidance of protein–DNA interaction (for MNs, ZFNs, and TALENs) or RNA–DNA base-pairing (for CRISPR/Cas9 and CRISPR/Cpf1) 16,17 .…”

Section: Genome-editing Systemsmentioning

confidence: 99%

Genome editing for horticultural crop improvement

Hua

Lang

2019

Hortic Res

View full text Add to dashboard Cite

Horticultural crops provide humans with many valuable products. The improvement of the yield and quality of horticultural crops has been receiving increasing research attention. Given the development and advantages of genome-editing technologies, research that uses genome editing to improve horticultural crops has substantially increased in recent years. Here, we briefly review the different genome-editing systems used in horticultural research with a focus on clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9)-mediated genome editing. We also summarize recent progress in the application of genome editing for horticultural crop improvement. The combination of rapidly advancing genome-editing technology with breeding will greatly increase horticultural crop production and quality.

show abstract

“…Transgenic mice are suitable model systems for most research fields and have also contributed enormously to our mechanistic knowledge about mucosal and cutaneous PVs (reviewed in Doorbar et al, 2015 ; Lambert, 2016 ; Santos et al, 2017 ). Current genome editing technologies make them relatively easy to generate and to manipulate ( Rocha-Martins et al, 2015 ). The transgene, regularly containing the open reading frames (ORFs) of E6, E6/E7 or the complete early region of the selected HPV type is typically expressed under control of the keratin 14 promoter which facilitates constitutive oncogenic expression in cycling epithelial cells.…”

Section: How Do the Current Rodent Models For Cutaneous Hpvs Fit To Tmentioning

confidence: 99%

Cutaneous Papillomaviruses and Non-melanoma Skin Cancer: Causal Agents or Innocent Bystanders?

2018

View full text Add to dashboard Cite

There is still controversy in the scientific field about whether certain types of cutaneous human papillomaviruses (HPVs) are causally involved in the development of non-melanoma skin cancer (NMSC). Deciphering the etiological role of cutaneous HPVs requires – besides tissue culture systems – appropriate preclinical models to match the obtained results with clinical data from affected patients. Clear scientific evidence about the etiology and underlying mechanisms involved in NMSC development is fundamental to provide reasonable arguments for public health institutions to classify at least certain cutaneous HPVs as group 1 carcinogens. This in turn would have implications on fundraising institutions and health care decision makers to force – similarly as for anogenital cancer – the implementation of a broad vaccination program against “high-risk” cutaneous HPVs to prevent NMSC as the most frequent cancer worldwide. Precise knowledge of the multi-step progression from normal cells to cancer is a prerequisite to understand the functional and clinical impact of cofactors that affect the individual outcome and the personalized treatment of a disease. This overview summarizes not only recent arguments that favor the acceptance of a viral etiology in NMSC development but also reflects aspects of causality in medicine, the use of empirically meaningful model systems and strategies for prevention.

show abstract

From Gene Targeting to Genome Editing: Transgenic animals applications and beyond

Cited by 38 publications

References 186 publications

Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing

Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing

Genome editing for horticultural crop improvement

Cutaneous Papillomaviruses and Non-melanoma Skin Cancer: Causal Agents or Innocent Bystanders?

Contact Info

Product

Resources

About