Effect of essential oil from Citrus aurantium in maternal reproductive outcome and fetal anomaly frequency in rats

Volpato, Gustavo Tadeu; Francia-Farje, Luis Alberto Domingo; Damasceno, Débora Cristina; Oliveira, Renata Vinhas; Hiruma-Lima, Clélia Akiko; Kempinas, Wilma De Grava

doi:10.1590/0001-3765201520140354

Cited by 20 publications

(8 citation statements)

References 33 publications

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“…Generally speaking, Citrus EOs are non-toxic, non-mutagenic, and non-carcinogenic [ 8 ]. They are not hazardous in pregnancy and do not alter the maternal reproductive outcome [ 8 , 166 ]. Sweet orange, bitter orange, neroli, petitgrain, lemon, lime (both distilled and expressed), bergamot, and grapefruit oils have GRAS status [ 8 ].…”

Section: Safety Of Citrus Oilsmentioning

confidence: 99%

Biological Activities and Safety of Citrus spp. Essential Oils

Dosoky

Setzer

2018

IJMS

230

162

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Citrus fruits have been a commercially important crop for thousands of years. In addition, Citrus essential oils are valuable in the perfume, food, and beverage industries, and have also enjoyed use as aromatherapy and medicinal agents. This review summarizes the important biological activities and safety considerations of the essential oils of sweet orange (Citrus sinensis), bitter orange (Citrus aurantium), neroli (Citrus aurantium), orange petitgrain (Citrus aurantium), mandarin (Citrus reticulata), lemon (Citrus limon), lime (Citrus aurantifolia), grapefruit (Citrus × paradisi), bergamot (Citrus bergamia), Yuzu (Citrus junos), and kumquat (Citrus japonica).

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Section: Safety Of Citrus Oilsmentioning

confidence: 99%

Biological Activities and Safety of Citrus spp. Essential Oils

Dosoky

Setzer

2018

IJMS

230

162

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“…Following the collection of fetuses from the uterine horns, these were weighed and classified as small (SGA), adequate (AGA), or large (LGA) for gestational age (Moraes-Souza et al, 2017 ). The placentas were weighed to calculate the placental efficiency (fetal weight/placental weight) (Volpato et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

Congenital Anomalies Programmed by Maternal Diabetes and Obesity on Offspring of Rats

et al. 2021

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Embryo-fetal exposure to maternal disorders during intrauterine life programs long-term consequences for the health and illness of offspring. In this study, we evaluated whether mild diabetic rats that were given high-fat/high-sugar (HF/HS) diet presented maternal and fetal changes at term pregnancy. Female rats received citrate buffer (non-diabetic-ND) or streptozotocin (diabetic-D) after birth. According to the oral glucose tolerance test (OGTT), the experimental groups (n = 11 animals/group) were composed of non-diabetic and diabetic receiving standard diet (S) or HF/HS diet. High-fat/high-sugar diet (30% kcal of lard) in chow and water containing 5% sucrose and given 1 month before mating and during pregnancy. During and at the end of pregnancy, obesity and diabetes features were determined. After laparotomy, blood samples, periovarian fat, and uterine content were collected. The diabetic rats presented a higher glycemia and percentage of embryonic losses when compared with the NDS group. Rats DHF/HS presented increased obesogenic index, caloric intake, and periovarian fat weight and reduced gravid uterus weight in relation to the other groups. Besides, this association might lead to the inflammatory process, confirmed by leukocytosis. Obese rats (NDHF/HS and DHF/HS) showed higher triglyceride levels and their offspring with lower fetal weight and ossification sites, indicating intrauterine growth restriction. This finding may contribute to vascular alterations related to long-term hypertensive disorders in adult offspring. The fetuses from diabetic dams showed higher percentages of skeletal abnormalities, and DHF/HS dams still had a higher rate of anomalous fetuses. Thus, maternal diabetes and/or obesity induces maternal metabolic disorders that contribute to affect fetal development and growth.

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“…When a lack of visible implantation sites was observed, the uterine corns were stained with a preparation of 10% ammonium sulfate [ 24 ]. The fetuses and placentas were weighed to calculate the placental efficiency as fetal weight/placental weight [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Safety evaluation of a vaccine: Effect in maternal reproductive outcome and fetal anomaly frequency in rats using a leishmanial vaccine as a model

et al. 2017

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While the immunogenic potential of the vaccination against infectious diseases was extensively shown, data on the safety assessment of recombinant proteins in vaccine formulations administered during pregnancy are still scarce. In the current study, the antigenicity of a vaccine against leishmaniasis (based on Leishmania braziliensis recombinant protein peroxidoxin) during pregnancy and possible maternal reproductive outcomes and fetal anomalies after immunization with a leishmanial vaccine or adjuvant alone (Bordetella pertussis derived MPLA adjuvant) were assessed. Rats were mated and allocated in three groups: Control—rats received saline; Adjuvant—rats received the adjuvant MPLA, and Vaccine—rats received the combination of MPLA and peroxidoxin. The administration was subcutaneously at the dorsal region, three times (days 0, 7, 14 of pregnancy). On day 21 of pregnancy, all rats were bled for biochemical and immunological measurements. The gravid uterus was weighed with its contents, and the fetuses were analyzed. The immunization with peroxidoxin induced a significant production of circulating IgG levels compared to other groups but caused a significant in post-implantation loss (14.7%) when compared to Control (5.0%) and Adjuvant (4.4%) groups. Furthermore, a significantly high rate of fetal visceral anomalies, such as hydronephrosis and convoluted ureter, was also observed in animals that received vaccine when compared to Control or Adjuvant groups. These data indicate the importance of safety evaluation of vaccines during pregnancy and the limited use of peroxidoxin administration during pregnancy. More importantly, the safety monitoring of immunization with MPLA derived from Bordetella pertussis demonstrated no reproductive outcomes associated with adjuvant administration, suggesting its safe use during pregnancy.

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Effect of essential oil from Citrus aurantium in maternal reproductive outcome and fetal anomaly frequency in rats

Cited by 20 publications

References 33 publications

Biological Activities and Safety of Citrus spp. Essential Oils

Biological Activities and Safety of Citrus spp. Essential Oils

Congenital Anomalies Programmed by Maternal Diabetes and Obesity on Offspring of Rats

Safety evaluation of a vaccine: Effect in maternal reproductive outcome and fetal anomaly frequency in rats using a leishmanial vaccine as a model

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