Antiproliferative action of aqueous extracts of Hymenaea stigonocarpa Mart. (Fabaceae) on the cell cycle of Allium cepa L.

Lacerda, Lourran P.; Malaquias, Geiz; Perón, Ana Paula

doi:10.1590/0001-3765201420130163

Cited by 25 publications

(27 citation statements)

References 7 publications

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“…This bioassay provides excellent proliferation kinetic properties and a few chromosomes (2n=16), which facilitates the detection of mitotic spindle changes and chromosomal breaks (16,17). It also allows the verifi cation of changes in the cell division or mitotic index when exposed to chemical compounds with potential cytotoxic action (3,16), and shows mostly a satisfactory similarity to results obtained in other bioassays (1,3).…”

Section: Introductionmentioning

confidence: 82%

Acute Toxicity of Grape, Plum and Orange Synthetic Food Flavourings Evaluated in in vivo Test Systems

Sales

Barbosa

Santos

et al. 2017

Food Technol. Biotechnol.

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SummaryThe present study evaluates the acute toxicity of synthetic grape, plum and orange fl avourings in root meristem cells of Allium cepa at the doses of 3.5, 7.0 and 14.0 mL/kg and exposure times of 24 and 48 h, and in bone marrow erythrocytes of mice treated orally for seven days with 0.5, 1.0, 2.0, 5.0 and 10.0 mL/kg of fl avouring. The results of the plant test showed that grape, plum and orange fl avourings, at both exposure times, inhibited cell division and promoted the formation of a signifi cant number of micronuclei and mitotic spindle changes. These alterations were observed in at least one exposure time analysed, demonstrating a signifi cant cytotoxic, genotoxic and mutagenic activity. In mouse bioassay, animals treated with 2.0, 5.0 and 10.0 mL/kg of fl avouring died before the seventh day of treatment. The amounts of 0.5 and 1.0 mL/kg of the three additives were cytotoxic to erythrocytes, and treatment with the grape fl avouring signifi cantly induced the formation of micronucleated cells in the bone marrow of animals. Therefore, under the study conditions, the grape, plum and orange fl avouring additives promoted signifi cant toxicity to cells of the test systems used.

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Section: Introductionmentioning

confidence: 82%

Acute Toxicity of Grape, Plum and Orange Synthetic Food Flavourings Evaluated in in vivo Test Systems

Sales

Barbosa

Santos

et al. 2017

Food Technol. Biotechnol.

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“…This test system is internationally accepted by research agencies as an assessment tool with accurate sensitivity for analysis of cytotoxicity, genotoxicity and mutagenicity of the substance of interest, since the results obtained mostly show satisfactory similarity to those obtained via animal testing systems and cell cultures (Herrero et al, 2012;Lacerda et al, 2014;Tabrez et al, 2011;Gomes et al, 2013;Dliveira et al, 2013;Campos-Ventura et al, 2016;Moura et al, 2016;Santana et al, 2016;Bezerra et al, 2016;Nunes et al, 2016).…”

Section: Introductionmentioning

confidence: 98%

Cytotoxic, genotoxic and mutagenic potential of UHT whole milk

2017

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This study evaluated the action at the cellular level of long life whole milk, full type of six renowned companies operating in the Brazilian market, as well as in other South American countries. The evaluation was performed using root meristem cells of Allium cepa L., at exposure times 24 and 48 hours, directly in milk products marketed. The results indicated that all the milk samples reduced root meristem cell proliferation, proving, in this study, a significant cytotoxic effect. Still, exposure to milk resulted in a significant frequency of mitotic spindle changes in meristem cells, characterizing these foods as genotoxic and mutagenic under the study conditions. It can be concluded that the long life milk samples caused significant genetic instability to cells of the examined tissue. The results obtained for cytotoxic, mutagenic and genotoxic action of these long life milks are of great relevance because, to date, there are no published toxicity studies on such foods and food additives present in the composition.

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“…The root meristem of Allium cepa L. (onion) is considered in the scientific milieu as an efficient bioassay for the evaluation of the acute toxicity at the cellular level of chemical compounds due to the reduced chromosome number (2n = 16), which favors detection of chromosomal or clastogenic alterations, mitotic spindle disturbances or aneugenic alterations, and disorders in the cell proliferation index. This test system is internationally accepted by research agencies as an assessment tool with accurate sensitivity for analysis of cytotoxicity, genotoxicity and mutagenicity of the substance of interest, since the results achieved demonstrate, in most cases, satisfactory similarity to those obtained though animal testing systems and in cell cultures Herrero et al [10], Neves et al [11], Lacerda et al [12], Tabrez et al [13]; Gomes et al [14], Oliveira et al [15]; CAMPOS & MarinMorales [16]; Moura et al [17], Santana et al [18].Therefore, the goal of this study was to evaluate the cytotoxicity and genotoxicity of diet shakes in root meristem cells of A. cepa, from three different highly commercialized brands in drugstores all over Brazil.…”

Section: Introductionmentioning

confidence: 98%

Cytogenotoxicity of Shakes Added with Synthetic Micro Ingredients

Peron¹

2017

MOJBM

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The goal of this study was to evaluate the cytotoxicity and genotoxicity of diet shakes of three brands widely marketed in Brazil, named in this study of A, B and C. Assessments were carried out on root meristem cells of Allium cepa at exposure times 24 and 48 hours. The products (treatments) for A and B were evaluated at concentrations of 0.05; 0.10 and 0.20 g/mL, and C at the concentration 0.08; 0.16 and 0.32 g/mL. The diet products at the three concentrations tested, including those suggested as ideal for consumption by the manufacturers, at the two exposure times considered, were found to have significant antiproliferative effect on the meristems evaluated. However, none of the treatments caused significant cellular changes to the tissues of roots. Thus, the products tested were cytotoxic, but not genotoxic, to root meristem cells of A. cepa. Furthermore, it was observed that in all the treatments performed, in the three concentrations, the majority of the dividing cells were at prophase, not being observed cells at anaphase and telophase. Thus, it is suggested to conduct more in-depth studies on the cytotoxicity of these shakes in physiologically more complex bioassays for the verification and deepening of the data obtained here.

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Antiproliferative action of aqueous extracts of Hymenaea stigonocarpa Mart. (Fabaceae) on the cell cycle of Allium cepa L.

Cited by 25 publications

References 7 publications

Acute Toxicity of Grape, Plum and Orange Synthetic Food Flavourings Evaluated in in vivo Test Systems

Acute Toxicity of Grape, Plum and Orange Synthetic Food Flavourings Evaluated in in vivo Test Systems

Cytotoxic, genotoxic and mutagenic potential of UHT whole milk

Cytogenotoxicity of Shakes Added with Synthetic Micro Ingredients

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