In vivo and in vitro propagation of “macela”: a medicinal-aromatic native plant with ornamental potential

Abstract

Achyrocline satureioides is a shrub native from South America. In popular medicine it is used in infusions such as digestive, carminative, antispasmodic, eupeptic and emmenagogue. However, its main use is as an ingredient in the liquor industry. Commercial exploitation is carried out through the collection of natural populations in an unsustainable way. The micropropagation of A. satureioides will allow its massive propagation and it will settle a base for its domestication. For this, a clone denominated as M1-5 was first propagated by cuttings. Subsequently, nodal segments obtained from young stems were disinfected by a standard method and cultured on MS medium. These shoots were used as a source of explants for subsequent assays. For its in vitro establishment MS medium and WPM were tested. Once the culture was established, the responses of the explants to increasing concentrations of 6-benzylaminopurine (BAP) (0.0; 0.5; 2.5 and 5.0 μM) with and without 0.05 μM α-naphthalene acetic acid (NAA) on WPM as basal medium were studied during 35 days. The proliferation of buds, the presence of callus and the number and length of the roots were evaluated. All of the “macela” cuttings in vivo propagated rooted and developed satisfactorily under the conditions tested. The application of 5.0 μM BAP alone generated the best multiplication rate, so it was selected as the multiplication medium. De novo shoots rooted spontaneously and finally, transferred to the greenhouse. Here in it was possible to establish a micropropagation protocol not only for the production of plantlets of selected clones but also for the application of biotechnological tools in the development of A. satureioides germplasm.

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

“…The suitability of the MS medium over WPM and B5 has also been reported by other workers, such as in Magnolia sirindhorniae (Cui et al, 2019), Aspilia africana (Okello et al, 2021), and Azadirachta indica (Bello et al, 2022). In contrast to our study, WPM was found as a suitable growth medium for woody tree species such as Achyrocline satureioides (Guariniello et al, 2018), Quercus aliena (Li et al, 2019), Hildegardia populifolia ( Upadhyay et al, 2020), and Alangium salviifolium (Pandey et al, 2022). Plant growth regulators interact with plant cells, tissues, and organs, and play an essential role in plant growth and development.…”

An in vitro Propagation of Aspilia africana (Pers.) C. D. Adams, and Evaluation of Its Anatomy and Physiology of Acclimatized Plants

“…Furthermore, a number of plants in the same family Asteraceae, including Aspilia mossambicensis ( Norton et al, 1993 ), Blumea mollis ( Tamilarasi and Thirugnanasampandan, 2014 ), Eupatorium triplinerve ( Janarthanam et al, 2011 ), and Achillea filipendulina ( Evenor and Reuveni, 2004 ), have also been successfully regenerated in MS medium. However, some studies have indicated that the growth of other plants, particularly the woody members of the Asteraceae family such as Achyrocline satureioides ( Guariniello et al, 2018 ) and Achyrocline flaccida ( Bonnecarrère et al, 2009 ), is better in WPM.…”