Crotalus durissus terrificus crotapotin naturally displays preferred positions for amino acid substitutions

Oliveira, Laudicéia Alves de; Ferreira, Rui Seabra; Barraviera, Benedito; Carvalho, Francilene Capel Tavares de; Barros, Luciana Curtolo de; Santos, Lucilene Delazari dos; Pimenta, Daniel C.

doi:10.1186/s40409-017-0136-5

Cited by 15 publications

(13 citation statements)

References 29 publications

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“…Differences in the composition and activity of snake venoms from the same species are a worldwide researchers concern. These differences can influence directly in the antivenom production and in the success of patient treatment [54][55][56][57].…”

Section: Resultsmentioning

confidence: 99%

Venom complexity of Bothrops atrox (common lancehead) siblings

Hatakeyama

Tasima

Bravo-Tobar

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Variability in snake venoms is a well-studied phenomenon. However, sex-based variation of Bothrops atrox snake venom using siblings is poorly investigated. Bothrops atrox is responsible for the majority of snakebite accidents in the Brazilian Amazon region. Differences in the venom composition of Bothrops genus have been linked to several factors such as ontogeny, geographical distribution, prey preferences and sex. Thus, in the current study, venom samples of Bothrops atrox male and female siblings were analyzed in order to compare their biochemical and biological characteristics. Methods: Venoms were collected from five females and four males born from a snake captured from the wild in São Bento (Maranhão, Brazil), and kept in the Laboratory of Herpetology of Butantan Intitute. The venoms were analyzed individually and as a pool of each gender. The assays consisted in protein quantification, 1-DE, mass spectrometry, proteolytic, phospholipase A 2 , L-amino acid oxidase activities, minimum coagulant dose upon plasma, minimum hemorrhagic dose and lethal dose 50%. Results: Electrophoretic profiles of male's and female's venom pools were quite similar, with minor sex-based variation. Male venom showed higher LAAO, PLA 2 and hemorrhagic activities, while female venom showed higher coagulant activity. On the other hand, the proteolytic activities did not show statistical differences between pools, although some individual variations were observed. Meanwhile, proteomic profile revealed 112 different protein compounds; of which 105 were common proteins of female's and male's venom pools and seven were unique to females. Despite individual variations, lethality of both pools showed similar values. Conclusion: Although differences between female and male venoms were observed, our results show that individual variations are significant even between siblings, highlighting that biological activities of venoms and its composition are influenced by other factors beyond gender.

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Section: Resultsmentioning

confidence: 99%

Venom complexity of Bothrops atrox (common lancehead) siblings

Hatakeyama

Tasima

Bravo-Tobar

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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“…The reaction was stopped adding 50% ACN/5% TFA and dried. Besides that, Tb II-I was chemically cleaved according to de the protocol described by Oliveira [ 66 ], using formic acid. All the reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Tb II-I, a Fraction Isolated from Tityus bahiensis Scorpion Venom, Alters Cytokines’: Level and Induces Seizures When Intrahippocampally Injected in Rats

Beraldo-Neto

Mariano

Freitas

et al. 2018

Toxins

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Scorpion venoms are composed of several substances with different pharmacological activities. Neurotoxins exert their effects by targeting ion channels resulting in toxic effects to mammals, insects and crustaceans. Tb II-I, a fraction isolated from Tityus bahiensis scorpion venom, was investigated for its ability to induce neurological and immune-inflammatory effects. Two putative β-sodium channel toxins were identified in this fraction, Tb2 II and Tb 4, the latter having been completely sequenced by mass spectrometry. Male Wistar rats, stereotaxically implanted with intrahippocampal cannulas and electrodes, were injected with Tb II-I (2 µg/2 µL) via the intrahippocampal route. The behavior, electrographic activity and cellular integrity of the animals were analyzed and the intracerebral level of cytokines determined. Tb II-I injection induced seizures and damage in the hippocampus. These alterations were correlated with the changes in the level of the cytokines tumoral necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Therefore, the binding of Tb II-I to its target in the central nervous system may induce inflammation resulting in neuropathological and behavioral alterations.

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“…In a recent study, de Oliveira et al. were able to discover multiple isoforms of crotapotin in the venom of the South American rattlesnake ( Crotalus durissus terrificus ) using de novo sequencing . Further recent studies have applied the technique other organisms, including snails, ants, scorpions, and spiders .…”

Section: Practical Applications Of De Novo Sequencing In Proteomicsmentioning

confidence: 99%

A Potential Golden Age to Come—Current Tools, Recent Use Cases, and Future Avenues for De Novo Sequencing in Proteomics

et al. 2018

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In shotgun proteomics, peptide and protein identification is most commonly conducted using database search engines, the method of choice when reference protein sequences are available. Despite its widespread use the database-driven approach is limited, mainly because of its static search space. In contrast, de novo sequencing derives peptide sequence information in an unbiased manner, using only the fragment ion information from the tandem mass spectra. In recent years, with the improvements in MS instrumentation, various new methods have been proposed for de novo sequencing. This review article provides an overview of existing de novo sequencing algorithms and software tools ranging from peptide sequencing to sequence-to-protein mapping. Various use cases are described for which de novo sequencing was successfully applied. Finally, limitations of current methods are highlighted and new directions are discussed for a wider acceptance of de novo sequencing in the community.

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Crotalus durissus terrificus crotapotin naturally displays preferred positions for amino acid substitutions

Cited by 15 publications

References 29 publications

Venom complexity of Bothrops atrox (common lancehead) siblings

Venom complexity of Bothrops atrox (common lancehead) siblings

Tb II-I, a Fraction Isolated from Tityus bahiensis Scorpion Venom, Alters Cytokines’: Level and Induces Seizures When Intrahippocampally Injected in Rats

A Potential Golden Age to Come—Current Tools, Recent Use Cases, and Future Avenues for De Novo Sequencing in Proteomics

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