Pediatric suppurative parotitis caused by Burkholderia pseudomallei

Fu, Zengzhen; Lin, Yuan; Wu, Qiang; Xia, Qianfeng

doi:10.1186/s40409-016-0086-3

Cited by 7 publications

(5 citation statements)

References 11 publications

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“…It is thus likely that Guangxi, and possibly Fujian, have been neglected as melioidosis-endemic, and improvements in awareness and diagnosis of the disease are needed to improve the understanding of its true prevalence in these provinces. In general, the range of clinical manifestations of melioidosis in China is similar to that seen elsewhere, with relatively rare clinical manifestations reported in the literature, including suppurative parotitis (one case) [43], osteomyelitis (three cases) [44] and pericarditis (one case) [45] from Hainan, as well as prostatic abscesses (two cases) [46] and co-infection with Japanese encephalitis (one case) [47] from Guangdong.…”

Section: Literature Reviewsupporting

confidence: 58%

Endemic Melioidosis in Southern China: Past and Present

Zheng

Xia

et al. 2019

TropicalMed

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Melioidosis is a severe tropical infectious disease caused by the soil-dwelling bacterium Burkholderia pseudomallei, predominantly endemic to Southeast Asia and northern Australia. Between the 1970s and the 1990s, the presence of B. pseudomallei causing melioidosis in humans and other animals was demonstrated in four coastal provinces in southern China: Hainan, Guangdong, Guangxi, and Fujian, although indigenous cases were rare and the disease failed to raise concern amongst local and national health authorities. In recent years, there has been a rise in the number of melioidosis cases witnessed in the region, particularly in Hainan. Meanwhile, although China has established and maintained an effective communicable disease surveillance system, it has not yet been utilized for melioidosis. Thus, the overall incidence, social burden and epidemiological features of the disease in China remain unclear. In this context, we present a comprehensive overview of both historical and current information on melioidosis in Southern China, highlighting the re-emergence of the disease in Hainan. Surveillance and management strategies for melioidosis should be promoted in mainland China, and more research should be conducted to provide further insights into the present situation.

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Section: Literature Reviewsupporting

confidence: 58%

Endemic Melioidosis in Southern China: Past and Present

Zheng

Xia

et al. 2019

TropicalMed

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“…As a facultative intracellular pathogen, it is the etiologic agent of melioidosis, nicknamed the "Vietnamese time bomb" as a result of American soldiers developing infections, sometimes many years after exposure to contaminated dust aerosolized by the helicopter rotor blades, or "the great mimicker" [1,2]. Melioidosis has a broad range of clinical manifestations, including pediatric suppurative parotitis, osteomyelitis, or meningoencephalitis, although the most frequent manifestation is pneumonia, either acute with sepsis or chronic, the latter which may be initially misdiagnosed as pulmonary tuberculosis due to similar signs and radiological appearances [3,4]. Acute forms have high mortality in some countries of Southeast Asia, which is strongly related to poverty.…”

Section: Introductionmentioning

confidence: 99%

A novel lytic phage potentially effective for phage therapy against Burkholderia pseudomallei in the tropics

Wang

Dance

et al. 2022

Infect Dis Poverty

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Background Burkholderia pseudomallei is a tropical pathogen that causes melioidosis. Its intrinsic drug-resistance is a leading cause of treatment failure, and the few available antibiotics require prolonged use to be effective. This study aimed to assess the clinical potential of B. pseudomallei phages isolated from Hainan, China. Methods Burkholderia pseudomallei strain (HNBP001) was used as the isolation host, and phages were recovered from domestic environmental sources, which were submitted to the host range determination, lytic property assays, and stability tests. The best candidate was examined via the transmission electron microscope for classification. With its genome sequenced and analyzed, its protective efficacy against B. pseudomallei infection in A549 cells and Caenorhabditis elegans was evaluated, in which cell viability and survival rates were compared using the one-way ANOVA method and the log-rank test. Results A phage able to lyse 24/25 clinical isolates was recovered. It was classified in the Podoviridae family and was found to be amenable to propagation. Under the optimal multiplicity of infection (MOI) of 0.1, an eclipse period of around 20 min and a high titer (1012 PFU/ml) produced within 1 h were demonstrated. This phage was found stabile at a wide range of temperatures (24, 37, 40, 50, and 60 °C) and pH values (3–12). After being designated as vB_BpP_HN01, it was fully sequenced, and the 71,398 bp linear genome, containing 93 open reading frames and a tRNA-Asn, displayed a low sequence similarity with known viruses. Additionally, protective effects of applications of vB_BpP_HN01 (MOI = 0.1 and MOI = 1) alone or in combination with antibiotics were found to improve viability of infected cells (70.6 ± 6.8%, 85.8 ± 5.7%, 91.9 ± 1.8%, and 96.8 ± 1.8%, respectively). A significantly reduced mortality (10%) and a decreased pathogen load were demonstrated in infected C. elegans following the addition of this phage. Conclusions As the first B. pseudomallei phage was isolated in Hainan, China, phage vB_BpP_HN01 was characterized by promising lytic property, stability, and efficiency of bacterial elimination during the in vitro/vivo experiments. Therefore, we can conclude that it is a potential alternative agent for combating melioidosis. Graphical Abstract

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“…Острый гнойный паротит часто встречается у детей в Юго-Восточной Азии, причем при отсутствии явных факторов риска, редко встречается у взрослых и ограничивается единичными случаями в Австралии. Мелиоидозный паротит может быть осложнен образованием абсцессов, разрывом слухового канала, параличом лицевого нерва и некротическим фасциитом, остео миелитом с септическим артритом и сепсисом [28,30,40].…”

Section: клинические формы и синдромы мелиоидозаunclassified

Melioidosis in aspects of epidemiology, clinic, and laboratory diagnostics

Zakharova¹,

Toporkov²,

Викторов³

2021

Russian Journal of Infection and Immunity

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Melioidosis is a life-threatening infection caused by Burkholderia pseudomallei, an environmental Gram-negative bacterium, inhabitant of moist soils in the tropics and subtropics. There is no licensed vaccine against melioidosis. The main routes of B. pseudomallei infection are percutaneous inoculation, inhalation, or ingestion. Individual cases of vertical, sexual, zoonotic, and nosocomial transmission of melioidosis are described. Risk factors for infection are contact with soil or water (especially during the rainy season). The age over 45, type 2 diabetes, alcoholism, liver disease, chronic lung disease, chronic renal disease, and thalassemia, as well as long-term use of steroids and immunosuppressive therapy, are the main susceptibility factors for melioidosis. Among the affected adult residents of endemic regions, 80% had one or more predisposing factors, among children — about 20%. No significant influence of concomitant diseases on the development of melioidosis in travelers was found. Less than 50% of patients had predisposing factors. The incubation period of melioidosis ranges within 1—21 days; on average, 9 days, in case of sizeable infectious dose, it can be less than one day. There is no post-infectious immunity, and reinfection can occur with a different B. pseudomallei strain after successful treatment. B. pseudomallei is a facultative intracellular pathogen that can invade and multiply inside a wide range of cells, including phagocytic. The acute form of melioidosis is characterized by pneumonia, multiple abscesses, bacteremia, and systemic sepsis. Chronic, subacute, and latent forms are also possible. Antimicrobial therapy is divided into the initial intensive phase and the subsequent eradication phase. B. pseudomallei is resistant to penicillins, first- and second-generation cephalosporins, aminoglycosides, macrolides, chloramphenicol, fluoroquinolones, tetracyclines, trimethoprim, and in some cases to co-trimoxazole, and rarely to ceftazidime. Early diagnosis and appropriate management are crucial in reducing severe complications leading to high mortality, and in preventing disease recurrences. However, there is no pathognomonic melioidosis-specific feature, and the disease is not well known to physicians and microbiologists. The results of serological tests for detection of specific antibodies are ambiguous. The bacterial load of the tested sample limits the detection of antigens. Among the accelerated methods for identifying the causative agent of melioidosis, PCR has the highest sensitivity and specificity. Automated identification using microbiological analyzers generally shows good results, but about 15% of isolates are misidentified. Time-of-flight mass spectrometry with matrix-assisted laser desorption ionization is potentially useful for rapid identification of B. pseudomallei. However, existing databases require optimization by adding the reference spectra for B. pseudomallei.

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Pediatric suppurative parotitis caused by Burkholderia pseudomallei

Cited by 7 publications

References 11 publications

Endemic Melioidosis in Southern China: Past and Present

Endemic Melioidosis in Southern China: Past and Present

A novel lytic phage potentially effective for phage therapy against Burkholderia pseudomallei in the tropics

Melioidosis in aspects of epidemiology, clinic, and laboratory diagnostics

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