Cellular transfer and AFM imaging of cancer cells using Bioimprint

Muys, James; Alkaisi, Maan M.; Melville, David O. S.; Nagase, Jison; Sykes, Peter; Parguez, Gaelle; Evans, John J.

doi:10.1186/1477-3155-4-1

Cited by 43 publications

(18 citation statements)

References 32 publications

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“…Unfortunately, probing the role of endocytosis on the targeting and transport of MNPs is very difficult in vivo , and only a few such studies on middle ear epithelium have been conducted. 36,37 Nevertheless, our observations suggest that TEM analyses could be performed to study how a magnetic field facilitates the targeting, transport and cellular uptake of MNPs in vivo . In our experiments, TEM revealed the formation of submicrometer-sized cell surface aggregates that were endocytosed, as well as the formation of large, cell surface MNP aggregates that were not endocytosed.…”

Section: Discussionmentioning

confidence: 93%

Pulsed Magnetic Field Improves the Transport of Iron Oxide Nanoparticles through Cell Barriers

Min

Shin

et al. 2013

ACS Nano

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Understanding how a magnetic field affects the interaction of magnetic nanoparticles (MNPs) with cells is fundamental to any potential downstream applications of MNPs as gene and drug delivery vehicles. Here, we present a quantitative analysis of how a pulsed magnetic field influences the manner in which MNPs interact with, and penetrate across a cell monolayer. Relative to a constant magnetic field, the rate of MNP uptake and transport across cell monolayers was enhanced by a pulsed magnetic field. MNP transport across cells was significantly inhibited at low temperature under both constant and pulsed magnetic field conditions, consistent with an active mechanism (i.e. endocytosis) mediating MNP transport. Microscopic observations and biochemical analysis indicated that, in a constant magnetic field, transport of MNPs across the cells was inhibited due to the formation of large (>2 μm) magnetically-induced MNP aggregates, which exceeded the size of endocytic vesicles. Thus, a pulsed magnetic field enhances the cellular uptake and transport of MNPs across cell barriers relative to a constant magnetic field by promoting accumulation while minimizing magnetically-induced MNP aggregates at the cell surface.

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Section: Discussionmentioning

confidence: 93%

Pulsed Magnetic Field Improves the Transport of Iron Oxide Nanoparticles through Cell Barriers

Min

Shin

et al. 2013

ACS Nano

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“…Protocols to analyse cell topologies with both scanning electron and transmission electron microscopy (SEM and TEM) are commonly used, however, such methods do not achieve a sufficient resolution for nanometre scale analysis. 51,62,63 Difficulties using AFM are also well documented, with a high pressure applied to cell membranes via the scanning cantilever, irreparable damage can be caused to the cells of the living tissue. Moreover, the deformation or the movement of living cell membranes can lead to imaging results that are not truly representative of the cell morphology.…”

Section: Nanoscale Cell Surface Analysismentioning

confidence: 99%

Cancer bioimprinting and cell shape recognition for diagnosis and targeted treatment

et al. 2017

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Cancer incidence and mortality have both increased in the last decade and are predicted to continue to rise. Diagnosis and treatment of cancers are often hampered by the inability to specifically target neoplastic cells. Bioimprinting is a promising new approach to overcome shortfalls in cancer targeting. Highly specific recognition cavities can be made into polymer matrices to mimic lock-and-key actions seen in in vivo biological systems. Early studies concentrated on molecules and were inhibited by template size complexity. Surface imprinting allows the capture of increasingly complex motifs from polypeptides to single cell organisms and mammalian cells. Highly specific cell shape recognition can also be achieved by cell interaction with imprints that can be made into polymer matrices to mimic biological systems at a molecular level. Bioimprinting has also been used to achieve nanometre scale resolution imaging of cancer cells. Studies of bioimprint-based drug delivery on cancer cells have been recently trialled in vitro and show that this approach can potentially improve existing chemotherapeutic approaches. This review focuses on the possible applications of bioimprinting with particular regards to cancer understanding, diagnosis and therapy. Cell imprints, incorporated into biosensors can allow the limits of detection to be improved or negate the need for extensive patient sample processing. Similar cell imprinting platforms can be used for nanoscale imaging of cancer morphology, as well as to investigate topographical signalling of cancer cells in vitro. Lastly, bioimprints also have applications as selective drug delivery vehicles to tumours with the potential to decrease chemotherapy-related side effects.

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“…Larger micronscale physiological cell-structures are clearly visible, such as connecting lamellipodia and the outline of the cell nucleus, as well as nanometer-sized detail on the cell-membrane, which has previously been attributed to exocytosis events and fusion pores. 4,28 However, the AFM scan of the same area after replication into casein, shown in Fig. 7(b), exhibits significantly less nanometer-scale detail.…”

Section: Replication Of Bioimprints Onto Casein Filmsmentioning

confidence: 99%

“…Mammalian cells cultured on engineered surfaces, with either, regular micro-and nanoscale patterns [1][2][3] or bioimprinted [4][5][6][7][8] cell-like features, have been shown to behave differently than cells cultured on flat surfaces. To this date, high-resolution surface patterning for biomedical applications has been confined to conventional tissue engineering plastics, such as polystyrene (PS), and specific metals used for medical implants.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of free-standing casein devices with micro- and nanostructured regular and bioimprinted surface features

Hashemi

Mutreja

Alkaisi

et al. 2015

Journal of Vacuum Science &Amp; Technology B, Nanotechnology and Microelectronics: Materials, Processing, Measurement, and Phen

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This work introduces a novel process for the fabrication of free-standing biodegradable casein devices with micro-and nanoscale regular and biomimetic surface features. Fabrication of intermediate polydimethylsiloxane (PDMS) moulds from photoresist masters and liquid-casting of casein is used to transfer arbitrary geometrical shapes onto the surface of casein devices. Casein film composition was optimized for mechanical stability and pattern resolution. It was found that 15% casein in 0.2% NaOH solution, mixed with 10% glycerol, and cross-linked by addition of 2% glutaraldehyde produced the best pattern transfer results. Biomimetic cell-like shapes were transferred onto casein by use of bioimprinting of two-dimensional cell-cultures into PDMS. To demonstrate this process, C2C12 mouse myoblasts were cultured on microscope slides, replicated into PDMS and casein using liquid casting and drying. Recessed alignment grids were integrated into the microscope glass slides to facilitate direct comparison of original cells and their bioimprints on PDMS and casein. Optical microscopy and atomic force microscopy confirmed the transfer of micron-scale morphological features, such as cell outlines, nuclei and larger lamellipodia, into the casein surface. Nanoscale feature resolution in casein was found to be limited compared to the PDMS intermediate moulds, which was attributed to limited wetting of the aqueous casein solution. Strategies to increase resolution of the casein transfer step, as well as degradation behavior of the fabricated devices in cell culture media are currently underway. Substrates fabricated with this process have applications in stem cell engineering, regenerative medicine, and implantable devices.

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Cellular transfer and AFM imaging of cancer cells using Bioimprint

Cited by 43 publications

References 32 publications

Pulsed Magnetic Field Improves the Transport of Iron Oxide Nanoparticles through Cell Barriers

Pulsed Magnetic Field Improves the Transport of Iron Oxide Nanoparticles through Cell Barriers

Cancer bioimprinting and cell shape recognition for diagnosis and targeted treatment

Fabrication of free-standing casein devices with micro- and nanostructured regular and bioimprinted surface features

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