“…Following exposure to the insecticide at 0.50 × 10 2 , 1.25 × 10 2 , and 2.5 × 10 2 µg a.i./L for 24, 48, and 72 h, surviving S. bipunctata individuals and insects not exposed to the pesticide were anesthetized under cold temperatures, and the midgut was dissected in solution (0.1 M NaCl, 0.1 M Na 2 HPO 4 , and 0.1 M KH 2 PO 4 ).The samples were fixed in aqueous Bouin's solution (picric acid, formaldehyde, and acetic acid) for 12 h, dehydrated in an alcohol series of increasing concentrations (70%, 80%, 90%, and 100%), diaphanized in xylene (100%), paraffin embedded, and sectioned into 6 μm slices using a microtome Leica RM 2250. Then, sections were spread on glass slides, rehydrated, and stained with Hematoxylin and eosin (H/E) . The analyses were performed under Olympus light microscope and sections were photographed using a digital camera.…”