Design of a luminescent biochip with nanodiamonds and bacterial luciferase

Puzyr, A. P.; Pozdnyakova, I. O.; Bondar, V. S.

doi:10.1134/1.1711469

Cited by 37 publications

(12 citation statements)

References 3 publications

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“…The construction of a biochip with a coating of nanodiamond particles carrying immobilised bacterial luciferase has also been reported (Figure 9). 54 There have also been activities towards the application of nanodiamond for gene and drug delivery into living cells. In a first example Kossovsky et al.…”

Section: Biological Applications Of Functionalised Nanodiamond Materialsmentioning

confidence: 99%

New Carbon Materials: Biological Applications of Functionalized Nanodiamond Materials

Krueger

2008

Chemistry A European J

397

286

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Nanoscale diamond particles have become an interesting material. Due to their inertness, small size and surface structure, they are well-suited for biological applications, such as labelling and drug delivery. Here we discuss the surface structure and functionalisation of diamond nanoparticles. Non-covalent as well as covalent grafting of bioactive moieties is possible, and first applications of fluorescent diamond nanoparticles are described.

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Section: Biological Applications Of Functionalised Nanodiamond Materialsmentioning

confidence: 99%

New Carbon Materials: Biological Applications of Functionalized Nanodiamond Materials

Krueger

2008

Chemistry A European J

397

286

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“…26,31,32 After selective functionalization, it can also serve for the detection of DNA oligonucleotides by using matrix assisted laser desorption/ ionization time-of-flight mass spectroscopy. 33 Because of the variety of applications offered by NDs in the field of biomedicine, their biocompatibility still remains an important topic. 33 Because of the variety of applications offered by NDs in the field of biomedicine, their biocompatibility still remains an important topic.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial effect of ultrafine nanodiamond against gram-negative bacteriaEscherichia coli

et al. 2014

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We investigate the antibacterial effect of ultrafine nanodiamond particles with an average size of 5 nm against the gram-negative bacteria Escherichia coli (E. coli). UV-visible, Raman spectroscopy, and scanning electron microscopy (SEM) have been employed to elucidate the nature of the interaction. The influence on bacterial growth was monitored by measuring optical densities of E. coli at 600 nm as a function of time in the presence of carboxylated nanodiamond (cND) particles (100 μg/ml ) in highly nutritious liquid Luria-Bertani medium. The SEM images prove that cND particles are attached to the bacterial cell wall surface and some portion of the bacterial cell wall undergoes destruction. Due to the change of the protein structure on the bacterial wall, a small Raman shift in the region of 1400 to 1700 cm⁻¹ was observed when E. coli interacted with cNDs. Raman mapping images show strong evidence of cND attachment at the bacterial cell wall surface. Electrotransformation of E. coli with a fluorescent protein markers experiment demonstrated that the interaction mechanisms are different for E. coli treated with cND particles, E. coli by lysozyme treatment, and E. coli that suffer lysis.

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“…The hydrogencarbon bonding characteristics of most diamond surfaces are stable (11) and convenient for bioconjugation either chemically (covalently or noncovalently) or physically (adsorption). Recently, the physical and chemical properties of nanometersized diamond particles have attracted great attention for their promising applications in the field of nanobiotechnology (12,13). The methods of functionalization and modification of different nanoparticles are developing (14); recently some work has been done concerning surface functionalization (15) and modification of diamond film surfaces with biomolecules such as DNA (5-7); different proteins, e.g., interacting antigens and antibodies (10,12), fluorescent dyelabeled proteins (16), etc., have been immobilized on the diamond thin films.…”

Section: Introductionmentioning

confidence: 99%

Nanometer-Sized Diamond Particle as a Probe for Biolabeling

Chao

Perevedentseva

Chung

et al. 2007

Biophysical Journal

253

174

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A novel method is proposed using nanometer-sized diamond particles as detection probes for biolabeling. The advantages of nanodiamond's unique properties were demonstrated in its biocompatibility, nontoxicity, easily detected Raman signal, and intrinsic fluorescence from its natural defects without complicated pretreatments. Carboxylated nanodiamond's (cND's) penetration ability, noncytotoxicity, and visualization of cND-cell interactions are demonstrated on A549 human lung epithelial cells. Protein-targeted cell interaction visualization was demonstrated with cND-lysozyme complex interaction with bacteria Escherichia coli. It is shown that the developed biomolecule-cND complex preserves the original functions of the test protein. The easily detected natural fluorescent and Raman intrinsic signals, penetration ability, and low cytotoxicity of cNDs render them promising agents in multiple medical applications.

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Design of a luminescent biochip with nanodiamonds and bacterial luciferase

Cited by 37 publications

References 3 publications

New Carbon Materials: Biological Applications of Functionalized Nanodiamond Materials

New Carbon Materials: Biological Applications of Functionalized Nanodiamond Materials

Antibacterial effect of ultrafine nanodiamond against gram-negative bacteriaEscherichia coli

Nanometer-Sized Diamond Particle as a Probe for Biolabeling

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