Bacterial Diversity in Rhizospheres of Nontransgenic and Transgenic Corn

Mao-Fa, Fang; Kremer, Robert J.; Motavalli, Peter P.; Davis, Georgia

doi:10.1128/aem.71.7.4132-4136.2005

Cited by 94 publications

(54 citation statements)

References 17 publications

(13 reference statements)

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“…Wu et al (2009) studied soil microbial communities in the rhizosphere of Bt and non-Bt rice using phospholipid fatty acid analysis, and found that Bt rice had no persistent effect on microbial community composition in the rhizosphere. Other studies showed that the effects of GM plants on microbial communities are subject more to seasonal variations or to other environmental factors than to expression of Cry or other proteins (Fang et al 2005, Icoz et al 2008. Although different effects, ranging from no effect to minor and significant effects, of different transgenic Bt plants on microbial communities and soil enzyme activities were reported (Gupta andWatson 2004, Rui et al 2005), most studies indicated that Bt rice have no or only minor effects, and effects are often transient in duration (Wu et al 2004b, Icoz et al 2008, Liu et al 2008.…”

Section: Resultsmentioning

confidence: 99%

Impact of Bt-transgenic rice (SHK601) on soil ecosystems in the rhizosphere during crop development

Wei¹,

Tan²,

Zhu³

et al. 2012

PLANT SOIL ENVIRON

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In contrast to other transgenic Bacillus thuringiensis (Bt) crops (e.g. Bt maize and cotton), risk assessments of Bt rice on soil ecosystem are few. To assess the influence of Bt rice on rhizosphere soil ecosystems, soil samples from Bt, non-Bt and controls were taken at seedling, tillering, booting, heading and maturing stages. The activities of dehydrogenases, invertase, phenol oxidases, acid phosphatases, ureases and proteases showed no significant differences between Bt and non-Bt rice. A Biolog system was used to evaluate the effect of Bt rice on functional diversity of microbial communities. Although there were differences in carbon substrate utilization between Bt and non-Bt rice at seedling, tillering and heading stages, these differences were transient and not persistent. Denaturing gradient gel electrophoresis (DGGE) fingerprint patterns showed that Bt rice had little effect on the dominant rhizosphere bacterial, fungal and actinobacterial communities. The richness and consistency of microbial communities according to carbon substrate utilizations and DGGE band patterns did not differ significantly between Bt and nonBt rice, and were close to that of control soil. There was no evidence to indicate apparent effects of Bt rice on soil enzyme activities, microbial community composition and functional diversity in this study.

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Section: Resultsmentioning

confidence: 99%

Impact of Bt-transgenic rice (SHK601) on soil ecosystems in the rhizosphere during crop development

Wei¹,

Tan²,

Zhu³

et al. 2012

PLANT SOIL ENVIRON

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“…Other studies demonstrated that bacterial diversity as measured by metabolic profiling and molecular analysis of 16S rRNAs in nontransgenic and transgenic corn rhizospheres was not significantly different (32,33). Indeed, rhizospheric bacterial diversity is affected more by soil texture or growing season than by cultivation of transgenic varieties (32,34).…”

Section: Bla Mutation Analysesmentioning

confidence: 99%

Antibiotic-resistant soil bacteria in transgenic plant fields

Demanèche

Sanguin

Poté

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

144

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Understanding the prevalence and polymorphism of antibiotic resistance genes in soil bacteria and their potential to be transferred horizontally is required to evaluate the likelihood and ecological (and possibly clinical) consequences of the transfer of these genes from transgenic plants to soil bacteria. In this study, we combined culture-dependent and -independent approaches to study the prevalence and diversity of bla genes in soil bacteria and the potential impact that a 10-successive-year culture of the transgenic Bt176 corn, which has a blaTEM marker gene, could have had on the soil bacterial community. The bla gene encoding resistance to ampicillin belongs to the beta-lactam antibiotic family, which is widely used in medicine but is readily compromised by bacterial antibiotic resistance. Our results indicate that soil bacteria are naturally resistant to a broad spectrum of beta-lactam antibiotics, including the third cephalosporin generation, which has a slightly stronger discriminating effect on soil isolates than other cephalosporins. These high resistance levels for a wide range of antibiotics are partly due to the polymorphism of bla genes, which occur frequently among soil bacteria. The blaTEM116 gene of the transgenic corn Bt176 investigated here is among those frequently found, thus reducing any risk of introducing a new bacterial resistance trait from the transgenic material. In addition, no significant differences were observed in bacterial antibiotic-resistance levels between transgenic and nontransgenic corn fields, although the bacterial populations were different.antibiotic resistance ͉ GMO ͉ HGT

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“…Previous studies showed that soil types influence the structure of microbial community, especially bacterial population among soils of different textures [16] [17]. Possible explanation for the higher number of bacteria in soil with caly contents was documented by Carney and Matson 2005 [18], who mentioned that fine textured soils support more microbial biomass than coarse textured soils.…”

Section: Resultsmentioning

confidence: 99%

Effect of Soil Physico-Chemical Properties and Plant Type on Bacterial Diversity in Semi-Arid Parts in Central Sudan. Part I: Omdurman North Region

Hassan¹,

EL-Kamali²

2015

OALib

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Bacterial diversity and total viable counts of bacteria of the different soil samples from two different localities in Omdurman north region-Karary and Khor Omer sub-regions-were carried out. Soil physical and chemical characteristics (pH, EC, SP, solublecations: Na, K, Ca, Mg and anion P, organic carbon, total nitrogen and soil texture) in each studied sub-regions were measured. Qualitative analysis of microorganisms isolated from the studied soil samples reveals a total of more than eight different species of bacteria, of which two are unidentified. The six species are classified under Bacillus genera. In Omdurman north region soil samples, total bacterial counts ranged from 7.5 × 10 3 cfu•g −1 to 1 × 10 4 with a mean of 4 × 10 4 cfu•g −1 . The quantitative data on microbial population recorded in the present study were analysed using two diversity indices. High Shannon-Weiner diversity index value for bacteria was obtained in Khor Omer sub-region (1.71261). Actinomyces spp. and Streptomyces spp. were the most abundant microorganisms identified in the two sub-regions. Total bacterial count in Karary soil was positively correlated with EC (r = 0.3868), clay (r = 0.1412), sand (r = 0.5891) and K (r = 0.0265) and negatively correlated with pH, silt, SP, Na, P, Ca, Mg, N and OC whereas the total bacterial count in Khor Omer soil was positively correlated with EC (r = 0.3973), clay (r = 0.1966), silt (r = 0.2116), Ca (r = 0.6733), Mg (r = 0.586) and OC (r = 0.2368) and negatively correlated with pH, sand, SP, Na, K, P and N. There were obvious differences in correlation coefficients among the selected criteria (37% of the total number of correlation coefficients were positively correlated between bacterial counts and soil physico-chemical properties whereas 63% of the total number were positively correlated between plant type and bacterial counts). The formulation of an appropriate national strategy in biotechnology should constitute an important, initial step towards the utilization and industrialization of microorganisms. The development of molecular techniques of microbial identification, coupled with traditional methods is promising areas for continued research.

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Bacterial Diversity in Rhizospheres of Nontransgenic and Transgenic Corn

Cited by 94 publications

References 17 publications

Impact of Bt-transgenic rice (SHK601) on soil ecosystems in the rhizosphere during crop development

Impact of Bt-transgenic rice (SHK601) on soil ecosystems in the rhizosphere during crop development

Antibiotic-resistant soil bacteria in transgenic plant fields

Effect of Soil Physico-Chemical Properties and Plant Type on Bacterial Diversity in Semi-Arid Parts in Central Sudan. Part I: Omdurman North Region

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