Influences of hyaluronic acid on the anticandidal activities of lysozyme and the peroxidase system

Kang, Jeong‐Hyun; Kim, Y.-Y.; Chang, Ju Yong; Kho, Hong‐Seop

doi:10.1111/j.1601-0825.2011.01807.x

Cited by 44 publications

(31 citation statements)

References 33 publications

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“…This disappearance comes with the concomitant surface CE . During the healing period of the current study, postoperative discomfort was scored by patient self‐assessment using two aforementioned parameters, pain and burning sensation after exogenous use of HA, introduced as a practical means to enhance wound healing with unique physiochemical and biologic properties and, thus, quality of life …”

Section: Discussionmentioning

confidence: 99%

“…Another major feature is viscoelasticity that provides stability and elasticity to tissues and delays penetration of viruses and bacteria . The highly biocompatible and non‐immunogenic nature of HA has bacteriostatic, fungistatic, anti‐inflammatory, antiedematous, osteoinductive, and proangiogenetic properties, leading to promotion of wound healing in a variety of tissues. Its properties have made HA of interest for treatment of various diseases in medical areas such as orthopedics, ophthalmology, and dermatology .…”

mentioning

confidence: 99%

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Effect of topically applied hyaluronic acid on pain and palatal epithelial wound healing: An examiner‐masked, randomized, controlled clinical trial

Yıldırım

Özener

Dogan

et al. 2018

Journal of Periodontology

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Effect of topically applied hyaluronic acid on pain and palatal epithelial wound healing: An examiner‐masked, randomized, controlled clinical trial

Yıldırım

Özener

Dogan

et al. 2018

Journal of Periodontology

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“…Synergistic action of lysozyme with other salivary proteins has been suggested by several authors as a mechanism of action towards C. albicans , and some oral care products already combine lysozyme with lactoferrin and lactoperoxidase (23–25). Indeed, in vitro investigations have shown that C. albicans susceptibility to lysozyme increases when combined with peroxidase (26,27) but not with lactoferrin (28), while hyaluronic acid inhibits the lysozyme effect on C. albicans (27,29). Lysozyme also enhances the activity of antimycotic drugs such as polyenes and azoles (30–33).…”

Section: Introductionmentioning

confidence: 99%

Dose-dependent effect of lysozyme upon Candida albicans biofilm

Sebaa

Hizette

Boucherit-Otmani

et al. 2017

Molecular Medicine Reports

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The present study investigated the in vitro effect of lysozyme (0–1,000 µg/ml) on Candida albicans (C. albicans) biofilm development. Investigations were conducted on C. albicans ATCC 10231 and on 10 clinical isolates from dentures. Strains were cultured aerobically at 37°C in Sabouraud broth. Yeast growth was evaluated by turbidimetry. Biofilm biomass was quantified on a polystyrene support by crystal violet staining and on acrylic surfaces by counts of colony forming units. Lysozyme affected biofilm formation to a greater extent than it affected growth. For the ATCC 10231 reference strain, lysozyme acted as a biofilm promotor on polystyrene at the highest concentration tested (1,000 µg/ml, non-physiological). When the reference strain was investigated on acrylic resin support, lysozyme acted as a significant biofilm promotor on rough resin, but less on smooth resin. The attached biomass in the presence of physiological concentrations of lysozyme (10–30 µg/ml) was significantly decreased compared with the hypothetical value of 100% using a one-sample t-test, but a comparison between the different lysozyme conditions using analysis of variance and post hoc tests did not reveal significant differences. In 10 wild strains, different patterns of biofilm formation on polystyrene were observed in the presence of lysozyme. Some strains, characterized by large amounts of biofilm formation in the presence of 1,000 µg/ml lysozyme, were poor biofilm producers at low concentrations of lysozyme. In contrast, some strains that were poor biofilm producers with a high lysozyme concentration were more inhibited by low concentrations of lysozyme. The present study emphasizes the need to develop strategies for biofilm control based on in vitro experiments, and to implement these in clinical trials prior to approval of hygiene products enriched with exocrine proteins, such as lysozyme. Further studies will extend these investigations to other Candida species, and to fungi and bacteria present in oral biofilms.

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“…This enzyme is commercially interesting mainly due to its antimicrobial activity, because it damages bacterial cell walls by catalyzing the hydrolysis of 1,4‐β‐bonds between GlcNAc and muramic acid . This enzyme has several applications in pharmaceutical, food and veterinary industries as fungistatic, anti‐inflammatory, antitumor, antiviral, a natural food preservative, etc . Therefore, the search for efficient and scalable strategies for its purification involves both an academic and an industrial challenge.…”

Section: Introductionmentioning

confidence: 99%

Sulfanilic acid‐modified chitosan mini‐spheres and their application for lysozyme purification from egg white

Hirsch

Baieli

Urtasun

et al. 2017

Biotechnology Progress

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A cation exchange matrix with zwitterionic and multimodal properties was synthesized by a simple reaction sequence coupling sulfanilic acid to a chitosan based support. The novel chromatographic matrix was physico-chemically characterized by ss-NMR and ζ potential, and its chromatographic performance was evaluated for lysozyme purification from diluted egg white. The maximum adsorption capacity, calculated according to Langmuir adsorption isotherm, was 50.07 ± 1.47 mg g while the dissociation constant was 0.074 ± 0.012 mg mL . The process for lysozyme purification from egg white was optimized, with 81.9% yield and a purity degree of 86.5%, according to RP-HPLC analysis. This work shows novel possible applications of chitosan based materials. The simple synthesis reactions combined with the simple mode of use of the chitosan matrix represents a novel method to purify proteins from raw starting materials. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:387-396, 2018.

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Influences of hyaluronic acid on the anticandidal activities of lysozyme and the peroxidase system

Abstract: Hyaluronic acid possesses fungistatic activity but no candidacidal activity. HA showed inhibitory effects on the candidacidal activities of lysozyme and the peroxidase system.

Cited by 44 publications

References 33 publications

Effect of topically applied hyaluronic acid on pain and palatal epithelial wound healing: An examiner‐masked, randomized, controlled clinical trial

Effect of topically applied hyaluronic acid on pain and palatal epithelial wound healing: An examiner‐masked, randomized, controlled clinical trial

Dose-dependent effect of lysozyme upon Candida albicans biofilm

Sulfanilic acid‐modified chitosan mini‐spheres and their application for lysozyme purification from egg white

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