Review of Laboratory Findings for Patients with Chronic Fatigue Syndrome

Buchwald, Dedra; Komaroff, Anthony L.

doi:10.1093/clinids/13.supplement_1.s12

Cited by 117 publications

(35 citation statements)

References 50 publications

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“…In contrast, autoantibodies in CFS are typically present in titers in the range of 150 to 1:400. The titers of autoantibodies observed in our study might be a reflection of a low-level chronic activation of the immune system, as has been proposed by many investigators (3,4,7,8,37,38).…”

Section: Discussionsupporting

confidence: 78%

“…Several studies have shown various immunologic abnormalities, including the presence of autoantibodies, immune complexes, abnormal levels of immunoglobulin classes and subclasses, decreased natural killer cell function, increased numbers of B cells and activated B cells, and increased CD4-l-and CD8+ T lymphocytes containing activation markers, in patients with CFS (7). A recent study of laboratory findings in CFS patients demonstrated higher levels of circulating immune complexes, atypical lymphocytosis, elevated IgG levels, and the presence of antinuclear antibodies (ANA) in 15% of cases, versus 0% of control subjects (8).…”

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confidence: 99%

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High frequency of autoantibodies to insoluble cellular antigens in patients with chronic fatigue syndrome

Mikecz

Konstantinov

Buchwald

et al. 1997

Arthritis & Rheumatism

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Objective. To elucidate the humoral immune response in patients with chronic fatigue syndrome (CFS), by identification and characterization of autoantibodies.Methods. Initial immunofluorescence histochemistry studies of sera using human HEp-2 cell substrate were followed by antibody class subtyping and colocalization studies with reference antibodies. Association of CFS autoantigens with insoluble cellular components was determined by in situ extraction of soluble components and subsequent immunofluorescence histochemistry studies on the extracted cell substrate.Results. Of 60 CFS patients, 41 (68%) were positive for antinuclear antibodies. Localization of nuclear staining was found at the nuclear envelope (52%), in reticulated speckles (25%), in nucleoli (13%), and in dense fine speckles (5%). Twenty-eight CFS sera (47%) also had antibodies to cytoplasmic antigens. The major cytoplasmic staining pattern was of the intermediate filament type (35%). The observed nuclear envelope pattern of staining co-localized with lamina-associated polypeptide 2 (an integral nuclear membrane protein), the reticulated speckle pattern co-localized with nonsmall nuclear RNP splicing factor SC-35, and the Publication no. 9865MEM from The Scripps Research Institute.

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Section: Discussionsupporting

confidence: 78%

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confidence: 99%

High frequency of autoantibodies to insoluble cellular antigens in patients with chronic fatigue syndrome

Mikecz

Konstantinov

Buchwald

et al. 1997

Arthritis & Rheumatism

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“…A large number of immune abnormalities, including cytokine production in CFS, have been reported (Buchwald & Komaroff, 1991, Gupta & Vayuvegula, 1991, Gupta, 1992, Jone, 1991, Kalimas et al, 1990, Lloyd et al, 1989, Straus et al, 1989. The published data on cytokine levels are contradictory (Cheney et al, 1989, Lever et al, 1988, Straus et al, 1989Lloyd et al, 1994, Linde et al, 1992.…”

Section: Introductionmentioning

confidence: 99%

Cytokine production by adherent and non-adherent mononuclear cells in chronic fatigue syndrome

Gupta

Aggarwal

See

et al. 1997

Journal of Psychiatric Research

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Summary--lt has been suggested that cytokines play a role in certain clinical manifestations of chronic fatigue syndrome (CFS). In this study adherent (monocytes) and non-adherent (lymphocytes) mononuclear cells were stimulated in the presence or absence of phytohemagglutinin (PHA) or lipopolysaccharide (LPS), respectively, and supernatants were assayed for IL-6, TNF-~, and IL-10 by ELISA. IL-6 was also measured at the mRNA level by polymerase chain reaction. The levels of spontaneously (unstimulated) produced TNF-.7 by non-adherent lymphocytes and spontaneously produced IL-6 by both adherent monocytes and non-adherent lymphocytes were significantly increased as compared to simultaneously studied matched controls. The abnormality of IL-6 was also observed at mRNA level. In contrast, spontaneously produced IL-10 by both adherent and non-adherent cells and by PHA-activated non-adherent cells were decreased. This preliminary study suggests that an aberrant production of cytokines in CFS may play a role in the pathogenesis and in some of the clinical manifestations of CFS. ,<) 1997 Elsevier Science Ltd.

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“…It is impossible to cover all these aspects in a brief review. As well the volume cited above, detailed reviews already exist on psychological [3], microbiological [4], immunological [5], physical [6] and psychiatric treatments [7], and historical issues [8] surrounding CFS. This review is therefore concerned primarily with neurobiological issues, which have yet to be comprehensively reviewed elsewhere.…”

Section: Introductionmentioning

confidence: 99%