Dissecting Redox Biology Using Fluorescent Protein Sensors

Abstract

We explore how novel sensor variants may further add to the current momentum toward a novel mechanistic and integrated understanding of redox biology in vivo. Antioxid. Redox Signal. 24, 680-712.

Garlic oil polysulfides: H2S- and O2-independent prooxidants in buffer and antioxidants in cells

“…Our experiments with roGFP, arguably the "gold standard" indicator for ROS (50), show that garlic oil alone acted as a potent oxidant and was effective at concentrations as low as 1 M (Fig. 7).…”

The Roles of Mitochondrial Reactive Oxygen Species in Cellular Signaling and Stress Response in Plants

“…Probes that can be specifically placed in mitochondria, either through electrical potential-driven accumulation (e.g. MitoSOX Red; Miller et al, 2009;Li and Xing, 2011;Martin et al, 2013;Liu et al, 2014) or through genetic targeting for fluorescent protein sensors (Schwarzländer et al, 2016) have addressed the localization issue. However, their chemical specificity in mitochondria also remains a concern.…”

Chloroplast-Specific in Vivo Ca2+ Imaging Using Yellow Cameleon Fluorescent Protein Sensors Reveals Organelle-Autonomous Ca2+ Signatures in the Stroma

“…S2). This was indicative of strong transgene silencing, which we and others have already observed in other fluorescent protein lines (Deuschle et al, 2006;Jones et al, 2014;Behera et al, 2015;Schwarzländer et al, 2016). To overcome this limitation, we introduced the 35S:2Bam4-YC3.6 construct into the Arabidopsis rdr6-11 background (here referred to as rdr6 for simplicity), which is impaired in gene silencing (Peragine et al, 2004).…”