cDNA sequence analysis of a 29-kDa cysteine-rich surface antigen of pathogenic Entamoeba histolytica.

Torian, Bruce E.; Flores, B M; Stroeher, V L; Hagen, Frederick S.; Stamm, Walter E.

doi:10.1073/pnas.87.16.6358

Cited by 75 publications

(64 citation statements)

References 31 publications

(19 reference statements)

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“…The galactose lectin is the second cysteine-rich surface antigen identified in E. histolytica (9). Giardia lamblia, another enteric protozoan parasite, also has several cysteinerich surface proteins (27,28).…”

Section: Discussionmentioning

confidence: 99%

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Sequence of a cysteine-rich galactose-specific lectin of Entamoeba histolytica.

Mann

Torian

Vedvick

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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125

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Entamoeba histolylica trophozoites adhere to human colonic mucins and epithelial cells by a cell surface galactose-specific lectin, This lectin, which is composed of two subunits linked by disulffide bonds, has been shown to be a protective antigen in an animal model of amebiasis. We have determined the sequence of the mature form of the 170-kDa heavy subunit from cDNA clones and PCR-amplifled fragments. The heavy subunit sequence consisted of a putative extracellular domain containing 1209 amino acids with 16 potential sites for N-linked glycosylation, a 26-amino acid hydrophobic region, and a 41-amino acid cytoplasmic tail. The presence of N-linked oligosaccharides was confirmed by culturing amebae with tunicamycin, which resulted in a decrease in the heavy subunit molecular mass to 160 kDa and a loss of lectin activity. The extracellular domain was remarkable for an extensive cysteine-rich domain that shared identity with similar regions of several other cell surface proteins and appeared to confer protease resistance to the subunit.

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Section: Discussionmentioning

confidence: 99%

“…Construction of an E. histolytica cDNA Agtll library from strain H-302:NIH has been described elsewhere (9). Recombinant bacteriophage was grown on Petri plates and overlaid with a nitrocellulose filter that had been soaked in isopropyl f-Dthiogalactopyranoside as described in the ProtoBlot protocol (Promega) and in ref.…”

mentioning

confidence: 99%

Sequence of a cysteine-rich galactose-specific lectin of Entamoeba histolytica.

Mann

Torian

Vedvick

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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125

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“…Two other ORFs are each similar to several proteins in the Swiss-Prot and GenPept data bases, but still defy identification. ORF 199 is similar to a mouse erythroleu kemia cell line-specific protein (MER5) (Yamamoto et al, 1989), a Helicobacterpyloriantigen (OToole et al, 1991), an Entamoeba histolytica surface antigen (Torian et al, 1990), and Salmonella typhimurium alkyl hydroperoxide reductase (Tartaglia et al, 1990). ORF 587 is similar to a group of eukaryotic proteins, Paslp, Secl8p, NSF, Cdc48p, and VCP, that have a conserved domain involved in ATP hydrolysis (Erdmann et al, 1991).…”

Section: Miscellaneous Genesmentioning

confidence: 99%

A High-Resolution Gene Map of the Chloroplast Genome of the Red Alga Porphyra purpurea.

1993

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Extensive DNA sequencing of the chloroplast genome of the red alga Porphyra purpurea has resulted in the detection of more than 125 genes. Fifty-eight (approximately 46%) of these genes are not found on the chloroplast genomes of land plants. These include genes encoding 17 photosynthetic proteins, three tRNAs, and nine ribosomal proteins. In addition, nine genes encoding proteins related to biosynthetic functions, six genes encoding proteins involved in gene expression, and at least five genes encoding miscellaneous proteins are among those not known to be located on land plant chloroplast genomes. The increased coding capacity of the P. purpurea chloroplast genome, along with other characteristics such as the absence of introns and the consenration of ancestral operons, demonstrate the primitive nature of the f . purpurea chloroplast genome. In addition, evidence for a monophyletic origin of chloroplasts is suggested by the identification of two groups of genes that are clustered in chloroplast genomes but not in cyanobacteria. INTRODUCTIONThe complete DNA sequences of three photosynthetic land plant chloroplast genomes have revealed an enormous amount of functional and evolutionary information. The chloroplast genomes of tobacco (Shinozaki et al., 1986), rice (Hiratsuka et al., 1989), and the liverwort Marchantia polymorpha (Ohyama et al., 1986) have each been shown to contain 110 to 118 genes. The products of these genes are primarily involved in two processes: gene expresssion (59 to 60 genes) and photosynthesis (29 genes). In addition, 11 genes encoding subunits of NADPH dehydrogenase (Arizmendi et al., 1992) as well as a number of conserved open reading frames (ORFs) are contained on these genomes. The gene content of all land plant chloroplast genomes investigated is surprisingly conserved (for review, see Palmer, 1991).Alga1 chloroplast genomes, on the other hand, have not been so extensively characterized. Current information on the chloroplast genomes of chlorophyll a/b-containing algae suggest that their gene content is not too different from that of land plants, although a few genes that are absent from land plant chloroplast genomes (e.g., tufA in several species [Baldauf et al., 19901, rpl5 [Christopher and Hallick, 19891, and ccsA [Orsat et al., 19921 in Euglena gracilis) have been identified. Of greater significance, however, may be the observation of substantial genome rearrangements in green alga1 chloroplast genomes in the form of the splitting up of ancestral operons in several species of Chlamydomonas (e.g., Woessner et al., 1987) and the large number of introns, including introns within introns, To whom correspondence should be addressed. in E. gracilis (Christopher and Hallick, 1989; Copertino and Hallick, 1991). These observations are indicative of chloroplast genomes that are evolving under more relaxed constraints than land plant chloroplast genomes. lnformation about the gene content of chloroplast genomes from chromophyte (chlorophyll a/c-containing) and rhodophyte/glaucophyte (c...

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“…Mice were given two booster injections with 100 p.g of OmpF trimers at 3-week intervals. The cell fusion and cloning by limiting dilution were performed as described by Torian et al (41,42). Screening was performed by ELISA with purified OmpF trimers and monomers at 1 p.g per well.…”

Section: Methodsmentioning

confidence: 99%

Acquisition of apparently intact and unmodified lipopolysaccharides from Escherichia coli by Bdellovibrio bacteriovorus

et al. 1992

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The ability of Bdellovibrio bacteriovorus to relocalize the OmpF major outer membrane porins from its Escherichia coli prey to its own outer membranes is diminished in prey expressing smooth lipopolysaccharide (S-LPS). Since porins exist in the membrane complexed with LPS, we examined the LPS associated with relocalized porin to determine whether it had been acquired intact, mixed or replaced with Edegovibrio LPS, or derivatized by the bdellovibrios. The relocalized trimers were found associated with the same LPS onrginally bound to them in the E. coli. The bulk-phase LPS from bdellovibrios grown on various chemotypes of rough prey was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine whether more than the trimer-bound LPS was acquired by the bdellovibrios. This analysis revealed bands ofBdeilovibrio LPS matching the LPS chemotype of the prey. One or two other bands were identical in migration to the LPS of prey-independent mutants of B. bacterovorus and represented bdellovibrio-synthesized LPS. The LPS of bdellovibrios grown on prey with radiolabeled lipid A showed radioactivity only in gel band positions identical with those of the prey's LPS. The amount-of this prey-derived LPS was shown by enzyme-linked immunosorbent assay to reach a constant value during the purification of the bdellovibrios, and it represented approximately 25% of the total Bdellovibrio LPS. Immunoelectron microscopy confirmed the presence of prey-derived LPS on the cell surface of bdellovibrios, and no evidence could be found for bdellovibrio-induced modifications of the relocalized prey LPS.

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cDNA sequence analysis of a 29-kDa cysteine-rich surface antigen of pathogenic Entamoeba histolytica.

Cited by 75 publications

References 31 publications

Sequence of a cysteine-rich galactose-specific lectin of Entamoeba histolytica.

Sequence of a cysteine-rich galactose-specific lectin of Entamoeba histolytica.

A High-Resolution Gene Map of the Chloroplast Genome of the Red Alga Porphyra purpurea.

Acquisition of apparently intact and unmodified lipopolysaccharides from Escherichia coli by Bdellovibrio bacteriovorus

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