Avaliação automatizada da infiltração e remoção celular em scaffolds descelularizados – Estudo experimental em coelhos

Santos, Alex de Lima; Silva, Camila Gonzaga da; Barreto, Leticia Siqueira de Sá; Tamaoki, Marcel Jun Sugawara; Almeida, Fernando G.; Faloppa, Flávio

doi:10.1055/s-0041-1739174

Cited by 2 publications

(1 citation statement)

References 33 publications

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“…In case of the larger structures, i.e., the rabbit gastrocnemius muscle tendon, after 2 weeks, the acellular graft was reseeded only peripherally. After 8 weeks, the graft was still noticeable but had significant progressive integration with native tissues [ 125 ]. Decellularized tendons are also a great solution for xenografts in ligament regeneration.…”

Section: Modifications Of Decm Scaffoldsmentioning

confidence: 99%

Decellularization of Dense Regular Connective Tissue—Cellular and Molecular Modification with Applications in Regenerative Medicine

Data,

Kulus,

Ziemak

et al. 2023

Cells

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Healing of dense regular connective tissue, due to a high fiber-to-cell ratio and low metabolic activity and regeneration potential, frequently requires surgical implantation or reconstruction with high risk of reinjury. An alternative to synthetic implants is using bioscaffolds obtained through decellularization, a process where the aim is to extract cells from the tissue while preserving the tissue-specific native molecular structure of the ECM. Proteins, lipids, nucleic acids and other various extracellular molecules are largely involved in differentiation, proliferation, vascularization and collagen fibers deposit, making them the crucial processes in tissue regeneration. Because of the multiple possible forms of cell extraction, there is no standardized protocol in dense regular connective tissue (DRCT). Many modifications of the structure, shape and composition of the bioscaffold have also been described to improve the therapeutic result following the implantation of decellularized connective tissue. The available data provide a valuable source of crucial information. However, the wide spectrum of decellularization makes it important to understand the key aspects of bioscaffolds relative to their potential use in tissue regeneration.

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Section: Modifications Of Decm Scaffoldsmentioning

confidence: 99%

Decellularization of Dense Regular Connective Tissue—Cellular and Molecular Modification with Applications in Regenerative Medicine

Data,

Kulus,

Ziemak

et al. 2023

Cells

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Decellularized biological matrices for the repair of rotator cuff lesions: a systematic review of preclinical in vivo studies

Codispoti,

Carniato,

Brogini

et al. 2024

Front. Bioeng. Biotechnol.

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Background: Rotator cuff tears (RCTs), resulting from degeneration or trauma of the shoulder tendons, are one of the main causes of shoulder pain. In particular, massive RCTs represent 40% of all injuries, require surgical treatment, and are characterized by poor clinical outcomes and a high rate of failure. In recent years, the use of biological decellularized patches for augmentation procedures has received great interest owing to their excellent self-integration properties, improving healing and, thus, presenting an innovative therapeutic option. However, the findings from clinical studies have emerged with conflicting viewpoints regarding the benefits of this procedure, as an excessive tension load might compromise the integrity of the tendon-to-bone connection when the patch exhibits low elasticity or insufficient strength. This could prevent the healing process, leading to unpredictable results in clinical practice.Methods: This systematic review was conducted following Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines across three databases (PubMed, Scopus, and Web of Knowledge) to underline the results obtained in preclinical studies involving animal models of RCT surgeries that utilized the biological decellularized matrix augmentation technique in the last 5 years.Results: Thirteen articles were included after the screening, and the SYRCLE tools were applied to assess the risk of bias in in vivo studies. Open-surgery techniques were conducted to create tendon defects or detachment in different animal models: rat (31%), rabbit (46%), dog (15%), and sheep (8%). Patches decellularized with non-standardized protocols were used in 77% of studies, while commercially available matrices were used in 15%. Of the studies, 31% used allogenic patches, 61% used xenogenic patches, and 8% utilized both xenogenic and autologous patches.Conclusion: Overall, this review provides a comprehensive overview of the use of acellular patches and their effective therapeutic potential in rotator cuff (RC) repair at the preclinical level with the aim of expanding the strategies and matrices available for surgeons.Systematic review registration:https://www.crd.york.ac.uk/prospero/, identifier CRD42023468716.

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Avaliação automatizada da infiltração e remoção celular em scaffolds descelularizados – Estudo experimental em coelhos

Cited by 2 publications

References 33 publications

Decellularization of Dense Regular Connective Tissue—Cellular and Molecular Modification with Applications in Regenerative Medicine

Decellularization of Dense Regular Connective Tissue—Cellular and Molecular Modification with Applications in Regenerative Medicine

Decellularized biological matrices for the repair of rotator cuff lesions: a systematic review of preclinical in vivo studies

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