Correlation between Serum Interleukin-17 level and Serum Reactive Oxygen Species levels among Children experiencing Otitis Media with Effusion

Abstract

Introduction The detection of inflammatory mediators in the serum of children with have otitis media with effusion (OME) and their correspondence with clinical considerations may enable the use of a modern nonsurgical curative treatment for OME. Objective To determine the relation between interleukin-17 (IL-17) serum level and reactive oxygen species (ROS) serum levels in children suffering from OME and to disclose if any variation occurs in the level of IL-17 Will affect the ROS and antioxidant equilibrium in the serum, which indicates the entire body's reaction to OME. Methods The present study was a case-control study. A total of 24 children experienced OME, and 24 healthy controls were recruited.All participants in the study were subjected to a systematic clinical investigation including otoscopic, audiometric, and tympanometric examination. Also, venous blood samples were collected from all children to determine the levels of IL-17 and ROS. Results The mean ± standard deviation (SD) age ranges of the patients and the control group were 6.8 ± 2.7 and 6.2 ± 3.4 years, respectively. A stylistically significant difference in the levels of serum nitric oxide (NO), catalase (CT), myeloperoxidase (MPO), and malondialdehyde (MDA) (p < 0.05) was detected between OME and control patients. No significant difference was found in serum levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) between OME and control patients. The serum levels of MDA, NO, and MPO positively correlated with the serum levels of IL-17 in OME patients. Conclusion In the present study, there is a reasonable role of the IL-17 pathway in OME pathogenesis through an increase in ROS levels.

Correlation between interleukin 17 (IL-17) levels and malondialdehyde (MDA) levels in patients with chronic suppurative otitis media

“…Based on the results of data analysis, it was found that there was a positive and significant correlation between Interleukin 17 (IL-17) levels and malondialdehyde (MDA) levels in the blood of patients with chronic suppurative otitis media (CSOM) with a high coefficient value of 0.623. This study is in line with the results concluding that the IL-17 pathway plays an important role in the pathogenesis of OME by increasing ROS, including MDA levels (Abdelhafeez, & Mohamed, 2021).…”

“…This is slightly different from previous literature which states that the percentage of CSOM patients is dominated by males, although there is no significant difference between male and female patients. This difference can be influenced by several factors, such as taking sample patients in an area where the proportion of males and females is quite different which will affect the percentage of CSOM patients seeking treatment at the health center where the study was conducted (Abraham et al, 2019;Abdelhafeez, & Mohamed, 2021). The type of hearing loss that is often experienced is CHL.…”

“…Lipoperoxidation (LPO) is the main reaction of reactive oxygen species (ROS), where this reaction involves polyunsaturated fatty acids which are then bound to phospholipid membranes where malondialdehyde (MDA) is the end product of lipid peroxidation induced by ROS (Shi, Wu, Li, Cai, Liu, Gao, & Chen, 2017). In line with these two studies, another study concluded that the IL-17 pathway plays an important role in the pathogenesis of acute otitis media through increasing ROS including MDA levels (Abdelhafeez, & Mohamed, 2021).…”

Dual red and near-infrared light-emitting diode irradiation ameliorates LPS-induced otitis media in a rat model

“…Excessive ROS production is associated with inflammation, aging, and human diseases (Snezhkina et al, 2019). In several human diseases, OM is correlated with ROS levels, and the IL-17 pathway is known to be associated with ROS levels in OM with effusion (Abdelhafeez and Mohamed, 2021). Specifically, ROS is associated with MAPK signaling, which is known as a master regulator of inflammation.…”