Premature ovarian insufficiency: A hormonal treatment approach

Benetti‐Pinto, Cristina Laguna; Júnior, José Maria Soares; Maciel, Gustavo Arantes Rosa; Nácul, Andréa Prestes; Yela, Daniela Angerame; Rosa‐e‐Silva, Ana Carolina Japur de Sá

doi:10.1055/s-0040-1716929

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…Different clinical symptoms such as amenorrhea, hypoestrogenism (estrogen reduction), and hyper-gonadotropism (increased levels of gonadotropins) can be manifested in POF women [6]. Until now, various pre-clinical strategies have been developed for the treatments of POF including hormone replacement therapy (HRT), gonadotropinreleasing hormone (GnRH) application, and modalities that are associated with the application of whole-cell, or cell-based products [7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Classical music restored fertility status in rat model of premature ovarian failure

Yamchi

Ashrafi

Abbasi

et al. 2022

BMC Complement Med Ther

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Background: The restorative effect of classical music was assessed on the cyclophosphamide-induced animal model of premature ovarian failure (POF). Methods: Mozart’s piano classical music (K.448) was used for up to 4 and 8 weeks. Rats were exposed to music 6 h every day using a stereo system with a volume of 65–70 dB. Sera and ovarian tissue samples were collected for the evaluation of FSH, LH, and E2 and histopathological examination. At the same time points, samples were taken from the hypothalamus and hippocampus to monitor the expression of Ntrk2, Crh, and Pomc using real-time PCR. Mating trial was performed to evaluate the fertility status of POF rats. Results: Histopathological examination revealed a significant increase (p < 0.05) in the numbers of morphologically normal follicles at all the developmental stages in POF rats after music therapy compared to the POF group (p < 0.05). Music therapy decreased FSH and LH levels to near-to-normal levels conidied with elevation of E2 (p < 0.05). Ntrk2, Crh, and Pomc expressions were down-regulated in POF rats. Music therapy increasaed the expression of Ntrk2 in the hypothalamus of POF rats (p < 0.05). In contrast, Crh and Pomc failed to reach the detection limit before intervention and four weeks after the intervention however, these genes were expressed eight weeks after music therapy. Fertility status was increased (p < 0.05) in terms of litter size in POF rats after being exposed to music compared to the non-treated POF control group (p < 0.05). Conclusion: Results showed that music can exert therapeutic effects on POF rats via the alteration of sex-related hormones.

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Section: Introductionmentioning

confidence: 99%

Classical music restored fertility status in rat model of premature ovarian failure

Yamchi

Ashrafi

Abbasi

et al. 2022

BMC Complement Med Ther

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show abstract

“…Fertility is markedly decreased though infertility of POI isn't absolute. The etiology of POI which is highly heterogeneous includes genetic, endocrine, autoimmune, metabolic, infectious, and iatrogenic factors [3].But about 90% of POI is considered idiopathic without known cause [4]. This had seriously hindered the clinical application in POI.…”

Section: Introductionmentioning

confidence: 99%

Profile genome-wide DNA methylation of human granulosa cells in primary ovarian insufficiency

Wang

Zhao

et al. 2022

Preprint

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Background Premature ovarian failure which leads to loss of fertility and low level of estrogen have serlously affected women's physical and mental health, especially, women of child-bearing age. The etiology remains unclear. DNA methylation, as a key epigenetic modification might play an importan role in primary ovarian insufficiency (POI). We explore the whole DNA methylation of POI by whole genome bisulfite sequencing (WGBS), and find the pattern of DNA methylation in POI and analyze the differentially methylated regions (DMRs) genes related POI. Methods According to clinical and experimental standards, follicular fluid samples were collected from three women with POI and three women with normal ovarian reserve (NOR). Ovarian granulosa cells were purified from each follicular fluid sample. Whole genomic DNA methylation sequencing of the granulosa cells was performed by WGBS. Results We obtained whole-genome methylation maps of human granulosa cells. The average percentages of methylated cytosines in CG contexts exceeded 99%, in CHG and CHH contexts was less than 1%. The average methylation level in CG was about 80%, in CHG and CHH was less than 0.4%. there was no significant difference in CpG methylation level between NOR and POI. But there were DMRs between two groups. Distribution of DMRs was enriched in promoter and exon. Differentially methylated genes were used to perform Gene ontology(GO) analysis and enriched in GO items of cellular process, biological regulation, metabolic process, developmental process,binding and catalytic activities. Conclusion DNA methylation plays a critical role in POI and serve as resources for further investigation in the future.

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“…Different clinical symptoms such as amenorrhea, hypoestrogenism (estrogen reduction), and hyper-gonadotropism (increased levels of gonadotropins) can be manifested in POF women [6]. Until now, various pre-clinical strategies have been developed for the treatments of POF including hormone replacement therapy (HRT), gonadotropin-releasing hormone (GnRH) application, and modalities that are associated with the application of whole-cell, or cell-based products [7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Classical music restored fertility status in rat model of premature ovarian failure

Nazdikbin

Ashrafi

Abbasi

et al. 2022

Preprint

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Background The restorative effect of classical music was assessed on the cyclophosphamide-induced animal model of premature ovarian failure (POF). Methods Mozart's piano classical music (K.448) was used for up to 4 and 8 weeks. Rats were exposed to music 6 hours every day using a stereo system with a volume of 65–70 dB. Sera and ovarian tissue samples were collected for the evaluation of FSH, LH, and E2 and histopathological examination. At the same time points, samples were taken from the hypothalamus and hippocampus to monitor the expression of Ntrk2, Crh, and Pomc using real-time PCR. Mating trial was performed to evaluate the fertility status of POF rats. Results Histopathological examination revealed a significant increase (p < 0.05) in the numbers of morphologically normal follicles at all the developmental stages in POF rats after music therapy compared to the POF group (p < 0.05). Music therapy decreased FSH and LH levels to near-to-normal levels conidied with elevation of E2 (p < 0.05). Ntrk2, Crh, and Pomc expressions were down-regulated in POF rats. Music therapy increasaed the expression of Ntrk2 in the hypothalamus of POF rats (p < 0.05). In contrast, Crh and Pomc failed to reach the detection limit before intervention and four weeks after the intervention however, these genes were expressed eight weeks after music therapy. Fertility status was increased (p < 0.05) in terms of litter size in POF rats after being exposed to music compared to the non-treated POF control group (p < 0.05). Conclusion Results showed that music can exert therapeutic effects on POF rats via the alteration of sex-related hormones.

show abstract

Premature ovarian insufficiency: A hormonal treatment approach

Cited by 4 publications

References 40 publications

Classical music restored fertility status in rat model of premature ovarian failure

Classical music restored fertility status in rat model of premature ovarian failure

Profile genome-wide DNA methylation of human granulosa cells in primary ovarian insufficiency

Classical music restored fertility status in rat model of premature ovarian failure

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