Comments on: Limitations of HPV DNA Testing in Screening of Cervical Adenocarcinomas

Concordance between the BD Onclarity and Roche cobas assays for detection of HPV DNA in a Chinese population

“…Therefore, HPV tests may be false-negative when L1 primers are used for PCR extraction efficiencies. 33 Given the close performance agreement, these factors are unlikely to be critical. 11 The transcription and translation of E6/E7 DNA play an important role in the persistence of HPV infection, 34 so there are many studies on E6/E7 messenger RNA (mRNA) and E6 protein as screening methods for cervical cancer.…”

“…When the HPV genome is integrated into the host genome, the L1 damaged HPV genome is destroyed and may be deleted. Therefore, HPV tests may be false‐negative when L1 primers are used for PCR extraction efficiencies 33 . Given the close performance agreement, these factors are unlikely to be critical 11 .…”

“…DNA concentration was determined via a Nanodrop (NanoDrop Technologies). HPV probes were designed according to full-length genome of 32 HPV types (6,11,16,18,26,30,31,33,34,35,39,40,42,43,44,45,51,52,53,55,56,58,59, 66, 67, 68, 69, 70, 73, 82, 85, and 97) by MyGenostics.…”