Effect of the Air Filtration System Replacement on Embryo Quality in the Assisted Reproduction Laboratory

Poletto, Karine Queiroz; Lima, Yanna Andressa Ramos de; Approbato, Mário Silva

doi:10.1055/s-0038-1670715

Cited by 3 publications

(2 citation statements)

References 34 publications

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“…The laboratory air quality is a factor that plays a s i g n i f i c a n t r o l e i n I V F o u t c o m e [ 4 , 9 , 4 7 ] . Improvements in the environmental conditions and air quality have been associated with overall positive effects on clinical outcomes [48]. The impact of volatile organic compounds (VOC) is well documented and have been pivotal to necessity of implementing air filtration system improvement measures, including replacing the air filtration system.…”

Section: Microorganisms From External Sourcesmentioning

confidence: 99%

“…The impact of volatile organic compounds (VOC) is well documented and have been pivotal to necessity of implementing air filtration system improvement measures, including replacing the air filtration system. Equally important are the actions to minimize the presence of microorganisms and subsequent contamination of cultures [47,48]. Given the importance of the air quality for an IVF laboratory, regulatory guidelines have been described by the European Union and Brazil to ensure specific requirements for air quality control [49,50].…”

Section: Microorganisms From External Sourcesmentioning

confidence: 99%

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Microbial contamination in assisted reproductive technology: source, prevalence, and cost

Borges

Berteli

Reis

et al. 2019

J Assist Reprod Genet

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Even the strictest laboratories and clinics are prone to the occurrence of microbial contamination. In the case of in vitro fertilization (IVF) research and practice facilities, the number of possible sources is particularly vast. In addition to ambient air, personnel, and non-sterilized materials, follicular fluid and semen from patients are a very common gateway for a diverse range of bacteria and fungi into embryo cultures. Even so, reports of contamination cases are rare, what leads many clinics to see the issue as a negligible risk. Microbiological contamination may result in the demise of the patient's embryos, leading to additional costs to both the patient and the clinics. Regardless of financial loss, emotional costs, and stress levels during IVF are highly distressing. Other worrisome consequences include DNA fragmentation, poor-quality embryos, early pregnancy loss or preterm birth, and possible long-term damages that need further investigation. In this review, we aimed to shed a light on the issue that we consider largely underestimated and to be the underlying cause of poor IVF outcomes in many cases. We also discuss the composition of the microbiome and how its interaction with the reproductive tract of IVF-seeking patients might influence their outcomes. In conclusion, we urge clinics to more rigorously identify, register, and report contamination occurrences, and highlight the role of the study of the microbiome to improve overall results and safety of assisted reproduction.

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Section: Microorganisms From External Sourcesmentioning

confidence: 99%

Section: Microorganisms From External Sourcesmentioning

confidence: 99%

Microbial contamination in assisted reproductive technology: source, prevalence, and cost

Borges

Berteli

Reis

et al. 2019

J Assist Reprod Genet

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Effects of Environmental EDCs on Oocyte Quality, Embryo Development, and the Outcome in Human IVF Process

Yang

2021

Advances in Experimental Medicine and Biology

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An updated view on the role of the female reproductive tract microbiome in IVF outcomes

Tursunova,

Lebedeva,

Altukhova

et al. 2023

Akušerstvo, ginekologiâ i reprodukciâ

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Introduction. In the last decade, a high-throughput 16S ribosomal RNA (rRNA) sequencing allowed to markedly extend insights into female reproductive tract microbiome. However, evidence about its role on in vitro fertilization (IVF) outcomes remains scarce and controversial.Aim: to analyze literature data for assessing an impact of the vaginal, uterine, and ovarian microbiome on IVF outcomes.Materials and Methods. The review was carried out based on publications from available in PubMed/MEDLINE, EBSCO, ResearchGate, Google Academy and еLibrary databases released over the last 20 years. For this, there was search for keywords and their combinations in Russian- and English-written publications: «vaginal microbiome», «cervical microbiome», «uterine microbiome», «ovarian microbiome», «microbiome of ovarian follicles», «IVF», «miscarriage», «early pregnancy loss», «implantation failure», «ovarian failure», «inflammasome». Only full-text original articles and reviews published in peer-reviewed journals were included in the review. Exclusion criteria were as follows: studies containing less than 10 observations per group, abstracts of conferences, studies on the male reproductive tract microbiome. Duplicate publicationswere excluded. The publications were selected independently by two co-authors, and in case of discrepancy two other co-authors were involved.Results. Vaginal, uterine, and ovarian microbiomes were characterized by providing relevant classifications and the features related to implantation failures and pregnancy loss after IVF. It was found that a decline in total Lactobacillus level and elevated proportion of L. jensenii, G. vaginalis, and Proteobacteria in the vaginal microbiome were predictors of implantation failure. In addition, IVF failure was also associated with the presence of Atopobium, Bifidobacterium, Chryseobacterium, Gardnerella, Streptococcus, Haemophilus, Staphylococcus, Brevundimonas, and Ralstonia in the uterine cavity as well as Bifidobacterium, Gardnerella, and Klebsiella in the endometrial tissue. On the other hand, Lactobacillus dominance in the uterine microbiome has a favorable effect. The colonization of the follicle by any microorganisms as well as the presence of follicular fluid anaerobic bacteria-derived metabolite trimethylamine-N-oxide (TMAO) associated with bacterial vaginosis additionally alters IVF outcomes. Moreover, the role of infectious cues in lowered ovarian reserve has also been established. Activation of the NLRP3 (NLR Family Pyrin Domain Containing 3) inflammasome by microbe-derived ligands stimulates production of pro-inflammatory cytokines and contributes to reduced follicle number. Blocking NLRP3 in mouse experiments can delay depletion of the follicle pool and result in elevated fertility.Conclusion. Favorable IVF outcomes are associated with Lactobacillus predominance in the vaginal and endometrial microbiome as well as lack of follicular fluid microorganisms. TMAO detected in the follicular fluid as well as activated NLRP3 inflammasome serve as negative predictors of IVF outcomes.

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Effect of the Air Filtration System Replacement on Embryo Quality in the Assisted Reproduction Laboratory

Cited by 3 publications

References 34 publications

Microbial contamination in assisted reproductive technology: source, prevalence, and cost

Microbial contamination in assisted reproductive technology: source, prevalence, and cost

Effects of Environmental EDCs on Oocyte Quality, Embryo Development, and the Outcome in Human IVF Process

An updated view on the role of the female reproductive tract microbiome in IVF outcomes

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