High Risk HPV E6/E7 Oncoprotein Expression in Women with High Grade Squamous Intraepithelial Lesion

Valença, J.E. Cordeiro; Gonçalves, Ana Katherine; Silva, Ismael Dale Cotrim Guerreiro da; Eleutério, José; Silva, Terezinha Tenório da; Bruneska, Danyelly; Ximenes, Ricardo Arraes de Alencar

doi:10.1055/s-0036-1580713

Cited by 6 publications

(5 citation statements)

References 26 publications

(17 reference statements)

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“…However, the antigen-processing was significantly impaired, which was measured by proteolytic cleavage of DQ-OVA and the activation of T cells by APCs [12]. Furthermore, there was a study that found that E7 mRNA expression was higher in women with high grade squamous intraepithelial lesion (HSIL) and cervical intraepithelial neoplasia (CIN) grade 2 or higher [13]. HPV E7 may also induce the upregulation of RRM2, which then promotes cervical carcinogenesis via ROS-ERK1/2-HIF-1α-VEGF-induced angiogenesis [14].…”

Section: Discussionmentioning

confidence: 99%

Production of Polyclonal Antibody to the HPV58 E7 Protein and Its Detection in Cervical Cancer

et al. 2016

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The persistent infection of high-risk human papillomavirus (HPV) is one of the most common causes of cervical cancer worldwide, and HPV type 58 is the third most common HPV type in eastern Asia. The E7 oncoprotein is constitutively expressed in HPV58-associated cervical cancer cells and plays a key role during tumorigenesis. To study the biological function of HPV58 E7 and to characterize E7 protein-host cell interactions, we cloned the human HPV58 E7 gene and produced specific E7 antibodies. The HPV58 E7 gene was cloned into a prokaryotic expression vector, pGEX-4T2. The recombinant plasmid pGEX-4T2-(HPV58-E7) was transformed into Escherichia coli DH5α and expressed as a fusion protein containing a GST tag. After purification and removal of the GST affinity tag, the E7 protein was used as an antigen for the production of antiserum in rabbits. The specificity of the purified HPV58 E7 antibody was detected by western blotting, immunofluorescence and immunohistochemistry analysis. These methods demonstrated that the polyclonal antibody could specifically recognize the endogenous and the recombinant HPV58 E7 proteins. Immunohistochemistry analysis indicated that the E7 protein was localized in the nucleus of cervical cancer cells.

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Section: Discussionmentioning

confidence: 99%

Production of Polyclonal Antibody to the HPV58 E7 Protein and Its Detection in Cervical Cancer

et al. 2016

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“… ∗ Excluding CIN2 from analysis. CIN: cervical intraepithelial neoplasia; CI: Confidence interval; DOR: diagnostic odds ratio; AUC: area under the curve; TP: true positive; FP: false positive; FN: false negative; TN: true negative.Included studies CIN1- vs. CIN2+: Aptima [8–35]; NucliSens EasyQ HPV [36–46]; OncoTect [41, 47–51]; PreTect HPV Proofer [14, 23, 25, 31, 52–61]; Quantivirus [45, 62–65]. CIN1- vs. CIN3+: Aptima [8–15, 18–22, 24, 26–31, 33–35]; NucliSens EasyQ HPV [36, 37, 41, 46]; OncoTect [41, 47–50]; PreTect HPV Proofer [14, 31, 59–61]; Quantivirus [62–64].…”

Section: Figurementioning

confidence: 99%

“…Included studies CIN1- vs. CIN2+: Aptima [8–35]; NucliSens EasyQ HPV [36–46]; OncoTect [41, 47–51]; PreTect HPV Proofer [14, 23, 25, 31, 52–61]; Quantivirus [45, 62–65]. …”

Section: Figurementioning

confidence: 99%

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Accuracy of mRNA HPV Tests for Triage of Precursor Lesions and Cervical Cancer: A Systematic Review and Meta-Analysis

Macedo¹,

Gonçalves²,

Bavaresco³

et al. 2019

Journal of Oncology

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Objective. This systematic review evaluates the accuracy of the mRNA HPV biomarker in cervical smears to identify cervical intraepithelial neoplasia (CIN) 2 or 3 and cervical cancer. Data Source. Eligible studies were identified by performing a search of electronic databases on Medline via Pubmed, Lilacs, Cochrane Library, Embase, and Grey literature for papers published between January 1990 and June 2018. Study Eligibility Criteria. As no randomized studies were identified, this review focuses on observational studies in which the mRNA HPV diagnostic test was compared to a histopathology reference standard. We analyzed studies that included women screened for cervical cancer using mRNA HPV. Study Appraisal and Synthesis Methods. After screening, 61 studies including 29,674 patients met the inclusion criteria and were analyzed. Dichotomization was performed by defining CIN2 or worse (CIN2+) versus CIN1, HPV infection, and normal (CIN 1-). The analysis was discriminated by the following tests: Aptima, PreTect HPV Profeer, NucliSens EasyQ HPV, OncoTect, and Quantivirus. Results. Analyzing by technique, Aptima, with 28 studies, exhibited superior performance, showing for the outcomes CIN2+ and CIN3+ an AUC of 0.88 (0.82-0.95) and 0.91 (0.84-0.99), a pooled sensitivity of 92.8% (95%CI 91.9-93.7) and 95.6% (95%CI 94.5-96.5), and a pooled specificity of 60.5% (95%CI 59.8-61.3) and 61.9% (95%CI 61.1-62.7), respectively. Conclusion. This study supports the current hypothesis that the mRNA HPV assay is an adequate tool for secondary cervical cancer screening.

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“…Our results showed that 55.6% of women with CIN2,3 had E6/E7 mRNA. In studies by Castle et al (22) and Valença et al (23), E6/E7 mRNA was detected in 84% and 70% of cases with a high-grade squamous intraepithelial lesion (HSIL), respectively. Negative results in CIN2,3 cases can be because of the low level or lack of transcriptional activity.…”

Section: Methodsmentioning

confidence: 97%

HPV E6/E7 mRNA detection in women with abnormal histology in Qazvin province, Iran

Benvari

Aslanimehr

Samiee-Rad

et al. 2023

Preprint

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Objective: Human papillomavirus (HPV) infection, especially with type 16 or 18, is associated with cervical cancer. Two HPV proteins, E6 and E7, are essential factors in the carcinogenesis of HPV. The purpose of this study was to evaluate the presence of HPV 16/18 E6/E7 mRNA in women with abnormal histology in Qazvin province, Iran. Results: HPV 16/18 DNA and E6/E7 mRNA was observed in 60.6% and 42.4% of samples, respectively. The frequency of positive HPV 16/18 and E6/E7 results increased with the severity of the injury, but the latter correlates better with progressive lesions. Despite the non-different sensitivity, PPV, and NPV of these two tests, E6/E7 mRNA showed higher specificity for detecting high-grade lesions (52.30% vs 75.38, P = 0.006).

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High Risk HPV E6/E7 Oncoprotein Expression in Women with High Grade Squamous Intraepithelial Lesion

Abstract: E6/E7 mRNA expression was higher in women with HSIL and CIN grade 2 or higher.

Cited by 6 publications

References 26 publications

Production of Polyclonal Antibody to the HPV58 E7 Protein and Its Detection in Cervical Cancer

Production of Polyclonal Antibody to the HPV58 E7 Protein and Its Detection in Cervical Cancer

Accuracy of mRNA HPV Tests for Triage of Precursor Lesions and Cervical Cancer: A Systematic Review and Meta-Analysis

HPV E6/E7 mRNA detection in women with abnormal histology in Qazvin province, Iran

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