A short-time treatment for isolating epicuticle from wool is described. Electron micrographs showing the penetration and disintegrating of the epicuticle caused by dilute Na 2 S or KOH are shown. These phenomena and their connection with the increased dyeing velocity and some other phenomena after treatment with alcoholic KOH are discussed.
Experimental ProcedureThe epicuticle of wool-that is, the thin membrane covering the fiber surface-was first isolated by treating wool with dilute Na2S at room temperature [5]. This procedure is, however, rather time-consuming, as it requires at least a month to carry out. A more rapid method, which yields samples of almost equal quality, is to treat wool with 1% Na-2S solution at 60°C for about 24 hrs. and then with a solution of the same concentration at room temperature for about 1 wk. Since after standing some time solutions of Na2S often will form precipitates or become discolored, it is recommended that the wool residue be washed after the elevated-temperature treatment so that impurities originating from the Na2S can he avoided.It is very difficult to get the epicuticle absolutely pure. Electron microscopy reveals that a great part of the membranes in a specimen can be obtained completely free from adhering substances, but that there will at the same time be some membranes with adhering bits of the exocuticle. Also larger particles, including elements from the endocuticle, can be found in the same specimen. Figure 1 is an electron micrograph of a piece of epicuticle, isolated by the short-time treatment and rather free from adhering substance. It has been shadowed with gold-manganin at an angle of 4: 1. (The wool had been subjected to a treatment with 2% KOH in ethyl alcohol for 5 min. before the Na2S treatment. This is irrelevant, as the same results have been ob-FIG. 1. Epicuticle) isolated by the short-tz1ne treatment with dilute Na2S, and rather free f rom adhering substances. Shadowed with gold-manganin.