Nucleic acid reassociation in formamide

McConaughy, Betty L.; Laird, Charles D.; McCarthy, Brian J.

doi:10.1021/bi00836a024

Cited by 587 publications

(202 citation statements)

References 11 publications

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“…2,000. The value obtained under identical conditions of reannealing for mouse unique fraction [17] is 1,800; these values are well within the limits of experimental error and indicate a maximum difference in sequence heterogeneity between these two fractions of 20%; that is, the "cytoplasmic" DNA contains essentially the same heterogeneity of intermediate and unique sequences as nuclear DNA in mouse.…”

Section: ~8supporting

confidence: 66%

“Cytoplasmic” DNA from primary embryonic cell cultures is not informational

Williamson¹,

McShane²,

Grunstein³

et al. 1972

FEBS Letters

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Section: ~8supporting

confidence: 66%

“Cytoplasmic” DNA from primary embryonic cell cultures is not informational

Williamson¹,

McShane²,

Grunstein³

et al. 1972

FEBS Letters

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“…The pH was then neutralized with 8 ml of a buffer solution (1 N NaCl, 1 N HCl, 1 M tris(hydroxymethy1)aminomethane = 2:l:l). The denatured DNA solution was passed at moderate speed (1 ml/3 min) at low temperature (3°C) through a nitrocellulose membrane filter (25- 29,1979 TAXONOMY OF CELLULOMONAS 275 tion conditions. Binding efficiency was determined by comparing the absorbance at 260 nm of the DNA solution before and after passage through the filter.…”

Section: Dna Hybridization (I) Preparation Of the Dnamentioning

confidence: 99%

“…The denaturation of the labeled DNA was carried out by boiling the DNA (10 pg/ml of 0.1X SSC) three times at 106°C in closed Pyrex tubes in an oil bath. The procedure for DNA-DNA hybridization followed that of McConaughy et al (29) with slight modifications. Filters with immobilized DNA were preincubated in the mixture described by Denhardt (13) and made up with 3X SSC.…”

Section: Dna Hybridization (I) Preparation Of the Dnamentioning

confidence: 99%

Taxonomy of the Genus Cellulomonas, Based on Phenotypic Characters and Deoxyribonucleic Acid-Deoxyribonucleic Acid Homology, and Proposal of Seven Neotype Strains

Stackebrandt¹,

Kandler²

1979

International Journal of Systematic Bacteriology

112

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Proposed as neotype strains of their respective species are the following:Cellulomonas biazotea ATCC 486, C. cellasea ATCC 487, C. fimi ATCC 484, C. flavigena ATCC 482, C. gelida ATCC 488, C. subalbus ATCC 489, and C. uda ATCC 491. Deoxyribonucleic acid-deoxyribonucleic acid reassociation studies on these and three other reference strains of Cellulomonas clearly revealed seven distinct species: C. biazotea, C. flavigena, C. cellasea, C. fimi, C. gelida, C. uda, and C. cartalyticum. C. subalbus ATCC 489 is identical with C. gelida ATCC 488 on the basis of genetic evidence. These results are confirmed by physiological data. C. subalbus is therefore a junior synonym of C. gelida. The polysaccharide and amino acid composition of the cell wall, the electrophoretic mobility of the L-lactate dehydrogenase, and the utilization of certain sugars and organic acids were found to be useful characters in species differentiation. Although the deoxyribonucleic acid-deoxyribonucleic acid homology values within the genus Cellulomonas range between 20 and 100%, those between strains of Cellulomonas and strains of Arthrobacter, Corynebacterium, and Brevibacterium are lower than 8%. These data, supported by the high guanine plus cytosine content of the DNA, the ability to decompose cellulose, and the similarity in the peptidoglycan types, demonstrate the coherence of the members of the genus Cellulomonas and the validity of the inclusion of this genus in the family Corynebacteriaceae. , and Bousfield 131) were restricted to a very limited number of strains and led to contradictory results. Some of the strains investigated by these authors were placed in other genera of the family Corynebacteriaceae even though they were strongly cellulolytic. According to the description of CellulomonasIn the present study, most of the currently available strains of Cellulomonas were characterized according to the type of peptidoglycan of their cell wall (14), the guanine plus cytosine (G+C) content of their deoxyribonucleic acid (DNA) (41), the fermentation pattern the serological properties (4). With respect to these characters, a relatively high degree of uniformity was noted with these strains. However, differences between the named species could clearly be demonstrated despite the fact that only one species was recognized in the last edition of Bergey's Manual (19), namely, Cellulomonas flavigena. In this paper the phenotypic characters of 10 reference strains, including seven proposed neotype strains, assigned to eight different named species are compared with the DNA-DNA homology data from these strains. The evidence indicates that the recognition of at least seven species in the genus Cellulomonas is justified. MATERIALS AND METHODS Bacterial

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“…High concentrations of certain electrolytes, including sodium perchlorate and trichloroacetates (1), as well as various neutral molecules, including formamide (2), dimethyl sulfoxide (3), and urea (4), lower the melting temperature of duplex DNA and therefore the optimum temperature for DNA:DNA reassociation (5,6). This is advantageous because, in general as the reaction temperature is lowered, the amount of chain scission occurring during the time needed for association reactions of complementary strands is reduced.…”

Section: Introductionmentioning

confidence: 99%