Developmental regulation of Tbx5 in zebrafish embryogenesis

Begemann, Gerrit; Ingham, Philip W.

doi:10.1016/s0925-4773(99)00246-4

Cited by 89 publications

(72 citation statements)

References 18 publications

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“…These markers include dorsally: ephrinB2A (25), msx-c (26), tbx5.1 (27), and BMP4 (28), and ventrally: pax2a (29) and vax2 (30). Whereas the direct functions of most of these molecules have yet to be well characterized within the retina, they are thought to play a role in compartment patterning, and serve as useful markers for the gross subdivision of the retina into dorsal and ventral compartments.…”

Section: Resultsmentioning

confidence: 99%

Tbx2b is essential for neuronal differentiation along the dorsal/ventral axis of the zebrafish retina

Gross

Dowling

2005

Proc. Natl. Acad. Sci. U.S.A.

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The mechanisms by which retinal neurons are patterned along the dorsal͞ventral axis remain largely unknown, yet this patterning is integral for the topographic mapping of visual space. With an interest in elucidating the mechanisms that regulate the development of this retinal axis, we have characterized a T-box family transcription factor, Tbx2b, during zebrafish retinogenesis. Tbx2b is expressed throughout all phases of retinal development with a striking asymmetry of distribution highest dorsally to lowest ventrally. To examine Tbx2b function during retinal development, two morpholino antisense oligonucleotides were created; one blocking the translational start site of Tbx2b and the other interfering with Tbx2b mRNA splicing. Injection of either of these morpholinos resulted in profound defects in the development of the dorsal retina. By using molecular markers for neuronal subtypes, the ventral retina contained all cell types, whereas in the dorsal retina, only retinal ganglion cells expressed markers of differentiation. The cells of the dorsal retina were postmitotic, however, as demonstrated by a lack of BrdUrd incorporation during the normal periods of retinal differentiation. Markers for dorsal and ventral retinal compartments were also expressed normally in Tbx2b morphants. Combined, these observations suggest that the cellular mechanisms regulating neuronal differentiation within the retina are asymmetric about the dorsal͞ventral axis and that Tbx2b mediates this process within the dorsal retina.T-box ͉ retinal development

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Section: Resultsmentioning

confidence: 99%

Tbx2b is essential for neuronal differentiation along the dorsal/ventral axis of the zebrafish retina

Gross

Dowling

2005

Proc. Natl. Acad. Sci. U.S.A.

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“…In situ hybridisation was performed as previously described (Begemann and Ingham, 2000). Probes used have been described previously: shha (Krauss et al, 1993), ihha (Qiao, 1996) ptc1 (Concordet et al, 1996), gli2a (Karlstrom et al, 1999), bmp4 (Hwang et al, 1997), prdm1 (Baxendale et al, 2004) and evx1 (Thaeron et al, 2000).…”

Section: Whole-mount In Situ Hybridisationmentioning

confidence: 99%

Hedgehog signalling is required for cloacal development in the zebrafish embryo

Parkin¹,

Allen²,

Ingham³

2009

Int. J. Dev. Biol.

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The Hedgehog (Hh) family of signalling molecules is essential for a wide range of developmental processes. Mammalian studies have implicated the Hedgehog pathway in the aetiology of anorectal malformations (ARMs), relatively common congenital anomalies caused by failures in the development of the cloaca. In this study we demonstrate that Hh signalling is absolutely required for the formation of the zebrafish cloaca and that the severity of the posterior gut abnormalities induced by a reduction in Hh activity is dependent on the levels of Hh signal transduction. The complete loss of all Hh activity results in the most severe defects and the critical period for Hh activity is between 34 and 74 hours post fertilisation. Using a range of mutant genotypes that cause notochord and floorplate abnormalities, we show that the source of the Hh signals required for posterior gut formation is the endoderm and not the notochord, as previously postulated in mammalian models of ARMs. We show that Adriamycin, a drug known to cause ARMs in rat, but not chick embryos, has no effect on the development of the zebrafish gastrointestinal tract. These studies establish the zebrafish as a model for ARMs, and for the elucidation of other pathways involved in hindgut developmental processes.

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“…4H,J,K, and data not shown). This lateral domain corresponds to the region where mesenchymal precursors of the pectoral fin buds are located (Begemann and Ingham, 2000). From 30 hpf onward, prtga hb, hindbrain; le, lens; lm, lateral mesoderm; mb, midbrain; ml, mantle layer; nt, neural tube; rp, roof plate; sc, spinal cord; tec, tectum; teg, tegmentum.…”

Section: Expression Patterns Of Zebrafish Prtga and Prtgbmentioning

confidence: 99%

Cloning of vertebrate Protogenin (Prtg) and comparative expression analysis during axis elongation

Vesque

Anselme

Couvé

et al. 2006

Developmental Dynamics

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A murine cDNA encoding Protogenin, which belongs to the DCC/Neogenin family, was cloned in a screen performed to identify novel cDNAs regionally expressed in the neural plate. Isolation of the putative zebrafish orthologues allowed a comparative analysis of the expression patterns of Protogenin genes during embryogenesis in different vertebrate species. From mid-gastrulation to early somite stages, Protogenin expression is restricted to posterior neural plate and mesoderm, with an anterior limit at the level of the rhombencephalon in mouse, chicken, and zebrafish. During somitogenesis, the expression profiles in the three species share features in the neural tube but present also species-specific characteristics. The initiation of Protogenin expression just before somitogenesis and its maintenance in the neural tube and paraxial mesoderm during this process suggest a conserved role in axis elongation. Developmental Dynamics 235:2836 -2844, 2006.

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Developmental regulation of Tbx5 in zebrafish embryogenesis

Cited by 89 publications

References 18 publications

Tbx2b is essential for neuronal differentiation along the dorsal/ventral axis of the zebrafish retina

Tbx2b is essential for neuronal differentiation along the dorsal/ventral axis of the zebrafish retina

Hedgehog signalling is required for cloacal development in the zebrafish embryo

Cloning of vertebrate Protogenin (Prtg) and comparative expression analysis during axis elongation

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