Identification of an oxygen responsive enhancer element in the glyceraldehyde-3-phosphate dehydrogenase gene

Graven, Krista K.; Yu, Qiang; Pan, David Z.; Roncarati, Jill S.; Farber, Harrison W.

doi:10.1016/s0167-4781(99)00118-9

Cited by 103 publications

(53 citation statements)

References 37 publications

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“…Expression of GAPDH, an enzyme involved in glycolysis, can be upregulated by physiological components in certain cell types; for example, by insulin in adipocytes (Ercolani et al, 1988) and by hypoxia in endothelial cells (Graven et al, 1999). Further investigation is needed to elucidate the molecular mechanisms sustaining high levels of transgene expression driven by the eIF-4A1 and GAPDH promoters in MSCs.…”

Section: Discussionmentioning

confidence: 99%

Sustained and promoter dependent bone morphogenetic protein expression by rat mesenchymal stem cells after BMP-2 transgene electrotransfer

Ferreira

Potier

Vaudin

et al. 2012

eCM

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Document VersionPublisher's PDF, also known as Version of Record (includes final page, issue and volume numbers) Please check the document version of this publication:• A submitted manuscript is the author's version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication Citation for published version (APA):Ferreira, E., Potier, E., Vaudin, P., Oudina, K., Bensidhoum, M., Logeart-Avramoglou, D., ... Petite, H. (2012). Sustained and promoter dependent bone morphogenetic protein expression by rat mesenchymal stem cells after bmp-2 transgene electrotransfer. European Cells and Materials, 24, 18-28. General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal ? Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. AbstractTransplantation of mesenchymal stem cells (MSCs) with electrotransferred bone morphogenetic protein-2 (BMP-2) transgene is an attractive therapeutic modality for the treatment of large bone defects: it provides both stem cells with the ability to form bone and an effective bone inducer while avoiding viral gene transfer. The objective of the present study was to determine the influence of the promoter driving the human BMP-2 gene on the level and duration of BMP-2 expression after transgene electrotransfer into rat MSCs. Cytomegalovirus, elongation factor-1α, glyceraldehyde 3-phosphate dehydrogenase, and beta-actin promoters resulted in a BMP-2 secretion rate increase of 11-, 78-, 66-and 36-fold over respective controls, respectively. In contrast, the osteocalcin promoter had predictable weak activity in undifferentiated MSCs but induced the strongest BMP-2 secretion rates in osteoblastically-differentiated MSCs. Regardless of the promoter driving the transgene, a plateau of maximal BMP-2 secretion persisted for at least 21 d after the hBMP-2 gene electrotransfer. The present study demonstrates the feasibility of gene electrotransfer for efficie...

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Section: Discussionmentioning

confidence: 99%

Sustained and promoter dependent bone morphogenetic protein expression by rat mesenchymal stem cells after BMP-2 transgene electrotransfer

Ferreira

Potier

Vaudin

et al. 2012

eCM

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“…Consistent with previous observations (42), we observed that expression of peptidylprolyl isomerase A/cyclophilin A (Cph) was significantly more constant between samples from different tissues and cultured cells than GAPDH expression (data not shown). In addition, GAPDH is not highly suitable for expression normalization in tumor studies, as it is a well characterized hypoxiaresponsive gene (43,44) with hypoxia-induced expression in cell lines and tumors (45). Thus, Cph expression was chosen as a constant internal reference standard to control for differences in RNA preparation and cDNA yield throughout this work.…”

Section: Methodsmentioning

confidence: 99%

Changes in the Expression of Many Ets Family Transcription Factors and of Potential Target Genes in Normal Mammary Tissue and Tumors

Galang

Muller

Foos

et al. 2004

Journal of Biological Chemistry

122

106

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The Ets family of transcription factors in mouse or humans is comprised of at least 26 unique family members that contain an evolutionarily conserved DNA binding domain called the Ets domain. The Ets family includes both transcriptional activators and repressors. These factors have been implicated as critical mediators of a wide range of cellular processes, including development, differentiation, growth, and transformation (reviewed in Ref.

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“…Indeed, the GAPDH gene and protein are actively regulated on cell proliferation. [19][20][21] GAPDH is the target of different transcription factors, and various control regions have been identified in its promoter, including hypoxia and insulinresponsive elements 22,23 ( Figure 1). In the context of cancer, hypoxia is of particular interest.…”

Section: Mechanisms Of Gapdh Regulationmentioning

confidence: 99%

Novel roles for GAPDH in cell death and carcinogenesis

2009

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Growing evidence points to the fact that glucose metabolism has a central role in carcinogenesis. Among the enzymes controlling this energy production pathway, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is of particular interest. Initially identified as a glycolytic enzyme and considered as a housekeeping gene, this enzyme is actually tightly regulated and is involved in numerous cellular functions. Particularly intriguing are recent reports describing GAPDH as a regulator of cell death. However, its role in cell death is unclear; whereas some studies point toward a proapoptotic function, others describe a protective role and suggest its participation in tumor progression. In this study, we highlight recent findings and discuss potential mechanisms through which cells regulate GAPDH to fulfill its diverse functions to influence cell fate.

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Identification of an oxygen responsive enhancer element in the glyceraldehyde-3-phosphate dehydrogenase gene

Cited by 103 publications

References 37 publications

Sustained and promoter dependent bone morphogenetic protein expression by rat mesenchymal stem cells after BMP-2 transgene electrotransfer

Sustained and promoter dependent bone morphogenetic protein expression by rat mesenchymal stem cells after BMP-2 transgene electrotransfer

Changes in the Expression of Many Ets Family Transcription Factors and of Potential Target Genes in Normal Mammary Tissue and Tumors

Novel roles for GAPDH in cell death and carcinogenesis

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