Endocrine disruption: thyroid dysfunction in mummichogs (Fundulus heteroclitus) from a polluted habitat

Zhou, Tong; John‐Alder, Henry B.; Weis, Judith S.; Weis, Peddrick

doi:10.1016/s0141-1136(00)00042-8

Cited by 38 publications

(25 citation statements)

References 10 publications

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“…The wild sea bass showed larger follicles formed by a thicker epithelium compared to the cultured sea bass. The follicle diameters measured in sea bass are in good agreement with those described previously for fish and mammals (Zhou et al, 2000).…”

Section: Thyroid Histologysupporting

confidence: 89%

“…The paraffin blocks were longitudinally sectioned (8 lm) through all the thyroid tissue (Zhou et al, 2000).…”

Section: Thyroid Histomorphometric Analysismentioning

confidence: 99%

“…The hematoxylin-eosin stain and the Van Gieson stain method were used for the microscopically diagnostic study of the histological samples (Zhou et al, 2000). These staining methods were perfectly suitable for this kind of study, since the follicles and the epithelial cells were clearly designable and different measurements could be taken.…”

Section: Thyroid Histomorphometric Analysismentioning

confidence: 99%

“…Several studies described adverse effects of organchlorinated contaminants on the thyroid function (Brouwer et al, 1989;Schumacher et al, 1993;Hall et al, 1998;Rolland, 2000;Zhou et al, 2000;Braathen et al, 2004;Brown et al, 2004;Debier et al, 2005;Das et al, 2006;Boas et al, 2006). The most innovative aspect of our approach is the simultaneous use of two alteration endpoints.…”

Section: Relationship Between Thyroid Parameters and Pcb Contaminationmentioning

confidence: 99%

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Effects of persistent organic pollutants on the thyroid function of the European sea bass (Dicentrarchus labrax) from the Aegean sea, is it an endocrine disruption?

Schnitzler¹,

Koutrakis²,

Siebert³

et al. 2008

Marine Pollution Bulletin

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Article history: Available online xxxx a b s t r a c tWe evaluated the alterations of organochlorinated compounds such as polychlorobiphenyls (PCB), dichloro-diphenyl-dichloroethylene (DDE) and dichloro-diphenyl-trichloroethane (DDT) on the thyroid in wild and cultured sea bass (Dicentrarchus labrax) at environmental concentrations. These compounds influence the endocrine system of many fish species and are qualified as endocrine disruptors. The thyroid seems to be a target organ. Two alteration endpoints: the thyroid histology and the muscular thyroid hormone concentrations, were used simultaneously. High concentrations in PCBs and DDT were detected in muscles, supporting the idea that the Mediterranean fauna could be more polluted than the Atlantic fauna. The high abundance of DDE indicates a progressive degradation of remnant DDT load and the absence of new inputs in this area. Aquaculture sea bass shows a significant higher amount of pollutants on fresh weight basis (especially PCBs) in their muscles compared to the wild sea bass. Those differences may be related mainly to the contaminations of diet. Thyroid parameters vary between wild and aquaculture sea bass, wild sea bass were characterized by higher follicle diameters, epithelial cell heights and muscular T 4 concentrations. A significant relationship between persistent organic pollutants (muscular PCBs and DDT concentration) and the different thyroid parameters (diameters of follicles, epithelial cell heights and muscular T 4 levels) could be observed, which support the hypothesis that these compounds have an adverse impact on thyroid morphometry and function.

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Section: Thyroid Histologysupporting

confidence: 89%

“…The paraffin blocks were longitudinally sectioned (8 lm) through all the thyroid tissue (Zhou et al, 2000).…”

Section: Thyroid Histomorphometric Analysismentioning

confidence: 99%

Section: Thyroid Histomorphometric Analysismentioning

confidence: 99%

Section: Relationship Between Thyroid Parameters and Pcb Contaminationmentioning

confidence: 99%

See 2 more Smart Citations

Effects of persistent organic pollutants on the thyroid function of the European sea bass (Dicentrarchus labrax) from the Aegean sea, is it an endocrine disruption?

Schnitzler¹,

Koutrakis²,

Siebert³

et al. 2008

Marine Pollution Bulletin

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show abstract

“…F. heteroclitus has been used as a model species for several recent studies of endocrine disruption. In particular, laboratory and natural populations exposed to environmental contaminants show altered levels of sex steroids (Dube & MacLatchy 2001, Hewitt et al 2002, MacLatchy et al 2003, Boudreau et al 2004, Greytak et al 2005, thyroid hormones (Zhou et al 2000, Carletta et al 2002, and aromatase mRNA (Greytak et al 2005). Given the cross-talk between mammalian ERs and ERRs (Vanacker et al 1999a, Giguere 2002, and the hypothesized regulatory role of ERRa in aromatase and thyroid receptor expression (Vanacker et al 1998, Yang et al 1998, elucidation of ERR signaling may provide insight into endocrine regulation and disruption in this model species.…”

Section: Introductionmentioning

confidence: 99%

Estrogen receptor-related receptors in the killifish Fundulus heteroclitus: diversity, expression, and estrogen responsiveness

Tarrant¹,

Greytak²,

Callard³

et al. 2006

Journal of Molecular Endocrinology

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The estrogen receptor-related receptors (ERRs) are a group of nuclear receptors that were originally identified on the basis of sequence similarity to the estrogen receptors. The three mammalian ERR genes have been implicated in diverse physiological processes ranging from placental development to maintenance of bone density, but the diversity, function, and regulation of ERRs in non-mammalian species are not well understood. In this study, we report the cloning of four ERR cDNAs from the Atlantic killifish, Fundulus heteroclitus, along with adult tissue expression and estrogen responsiveness. Phylogenetic analysis indicates that F. heteroclitus (Fh)ERRa is an ortholog of the single ERRa identified in mammals, pufferfish, and zebrafish. FhERRba and FhERRbb are co-orthologs of the mammalian ERRb. Phylogenetic placement of the fourth killifish ERR gene, tentatively identified as FhERRgb, is less clear. The four ERRs showed distinct, partially overlapping mRNA expression patterns in adult tissues. FhERRa was broadly expressed. FhERRba was expressed at apparently low levels in eye, brain, and ovary. FhERRbb was expressed more broadly in liver, gonad, eye, brain, and kidney. FhERRgb was expressed in multiple tissues including gill, heart, kidney, and eye. Distinct expression patterns of FhERRba and FhERRbb are consistent with subfunctionalization of the ERRb paralogs. Induction of ERRa mRNA by exogenous estrogen exposure has been reported in some mammalian tissues. In adult male killifish, ERR expression did not significantly change following estradiol injection, but showed a trend toward a slight induction (three-to five-fold) of ERRa expression in heart. In a second, more targeted experiment, expression of ERRa in adult female killifish was downregulated 2$5-fold in the heart following estradiol injection. In summary, our results indicate that killifish contain additional ERR genes relative to mammals, including ERRb paralogs. In addition, regulation of ERRa expression in killifish apparently differs from regulation in mammals. Together, these features may facilitate determination of both conserved and specialized ERR gene functions.

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2008

Histological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish

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Endocrine disruption: thyroid dysfunction in mummichogs (Fundulus heteroclitus) from a polluted habitat

Cited by 38 publications

References 10 publications

Effects of persistent organic pollutants on the thyroid function of the European sea bass (Dicentrarchus labrax) from the Aegean sea, is it an endocrine disruption?

Effects of persistent organic pollutants on the thyroid function of the European sea bass (Dicentrarchus labrax) from the Aegean sea, is it an endocrine disruption?

Estrogen receptor-related receptors in the killifish Fundulus heteroclitus: diversity, expression, and estrogen responsiveness

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