The translation state of differentially expressed mRNAs in the hibernating 13-lined ground squirrel (Spermophilus tridecemlineatus)

Hittel, Dustin S.; Storey, Kenneth B.

doi:10.1016/s0003-9861(02)00048-6

Cited by 85 publications

(93 citation statements)

References 24 publications

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“…This augmented capacity for LCFA flux was facilitated by increases in H-FABP protein in HFD compared with CHD. These data confirm previous studies showing H-FABP levels to increase with LCFA demand and utilization such as experimental diabetes (20), exercise training (21), weight loss (10), high-fat feeding (9), and hibernation (22)(23)(24). Given the highly variable nature of H-FABP and its role in facilitating LCFA flux, the aim of the present study was to examine whether a partial genetic deletion of H-FABP was protective toward the development of dietary-induced insulin resistance.…”

Section: Discussionsupporting

confidence: 91%

Partial Gene Deletion of Heart-Type Fatty Acid–Binding Protein Limits the Severity of Dietary-Induced Insulin Resistance

et al. 2005

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The aim of this study was to determine the contribution of heart-type fatty acid-binding protein (H-FABP) to glucose and long-chain fatty acid (LCFA) utilization in dietaryinduced insulin resistance. We tested the hypothesis that H-FABP facilitates increases in LCFA flux present in glucose-intolerant states and that a partial reduction in the amount of this protein would compensate for all or part of the impairment. Transgenic H-FABP heterozygotes (HET) and wild-type (WT) littermates were studied following chow diet (CHD) or high-fat diet (HFD) for 12 weeks. Catheters were surgically implanted in the carotid artery and jugular vein for sampling and infusions, respectively.

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Section: Discussionsupporting

confidence: 91%

Partial Gene Deletion of Heart-Type Fatty Acid–Binding Protein Limits the Severity of Dietary-Induced Insulin Resistance

et al. 2005

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“…For example, plasma membrane Na + K + -ATPase activity was reduced by 60% during hibernation (MacDonald and Storey, 1999) and the activity of sarco(endo)plasmic reticulum Ca 2+ -ATPase as well as the levels of other proteins associated with calcium signaling were similarly reduced (Malysheva et al, 2001). Strong overall suppression of ATP-expensive protein synthesis has also been confirmed in hibernators in both in vivo and in vitro studies that monitored 14 C-leucine or 3 H-leucine incorporation into proteins (Frerichs et al, 1998;Hittel and Storey, 2002;Osborne et al, 2004). Finally, the overall rates of gene transcription are also greatly reduced during deep torpor bouts in hibernators.…”

Section: Metabolic Suppression In Hibernationmentioning

confidence: 86%

“…For example, fatty acid binding proteins (FABPs) are up-regulated to enhance the capacity for intracellular transport of fatty acids which are the primary fuels for metabolism during torpor (Hittel and Storey, 2002) whereas up-regulation of pyruvate dehydrogenase kinase results in the inhibition of pyruvate dehydrogenase and the subsequent suppression and sparing of carbohydrate fuels (Andrews et al, 1998). Selective gene up-regulation is under the control of transcription factors that bind to response elements present in the promoter region of genes to enhance transcriptional activity of the gene.…”

Section: Transcriptional Control In Hibernationmentioning

confidence: 99%

“…One of these is the physical association of mRNA transcripts with monosomes or with ribonucleoprotein particles. One aspect of translational suppression during hibernation is polysome dissociation so that most ribosomes are sequestered into the translationally-silent monosome fraction during hibernation, taking with them a high proportion of cellular mRNA transcripts (Frerichs et al, 1998;Knight et al, 2000;Hittel and Storey, 2002). Only transcripts that are preferentially translated during hibernation, such as fabp (fatty acid binding protein) remained associated with polysomes (Hittel and Storey, 2002).…”

Section: Mirna Control Of Hibernation: the Next Venture?mentioning

confidence: 99%

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Mammalian hibernation: differential gene expression and novel application of epigenetic controls

Morin¹,

Storey²

2009

Int. J. Dev. Biol.

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This review highlights current information about the regulatory mechanisms that govern gene expression during mammalian hibernation, in particular the potential role of epigenetic controls in coordinating the global suppression of transcription. Hibernation is characterized by long periods of deep torpor (when core body temperature drops to near ambient) that are interspersed with brief arousal periods back to euthermia. Entry into torpor requires coordinated controls which strongly suppress and reprioritize all metabolic functions, including global controls on both transcription and translation. At the same time, however, selected hibernation-specific genes are up-regulated under the control of specific transcription factors to support the torpid state; this includes genes that encode proteins involved in lipid fuel catabolism and in long term cytoprotection (e.g. antioxidants, chaperones). We evaluate the currently available information on global transcriptional suppression in hibernation and propose that epigenetic mechanisms such as DNA methylation, histone modification, SUMOylation and the actions of sirtuins play crucial roles in transcriptional suppression during torpor. Global controls providing translational suppression also occur during hibernation including reversible phosphorylation control of ribosomal initiation and elongation factors as well as polysome dissociation. We also present initial data that mRNA transcripts are regulated via inhibitory interactions with microRNA species during torpor and provide the first evidence of differential expression of miRNAs in hibernators. When taken together, these mechanisms provide hibernators with multiple layers of regulatory controls that achieve both global repression of gene expression and selected enhancement of genes/proteins that achieve the hibernation phenotype.

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“…As such, the regulation of protein translation appears to be a protective response to deal with environmental stresses that ultimately disrupt cellular ATP production. Several in vivo studies have documented the decrease in protein translation rates during hypometabolism in anoxic red eared slider turtles (Brooks and Storey, 1993;Land et al, 1993;Fraser et al, 2001) and marine snails (Larade and Storey, 2002), as well as hibernating thirteen-lined ground squirrels (Frerichs et al, 1998;Hittel and Storey, 2002). One study found that the rate of protein translation in turtles following 1 h anoxia exposure (at 23°C) showed no significant changes from normoxic values.…”

Section: Control Of Protein Translationmentioning

confidence: 99%

Insight into post-transcriptional gene regulation: stress-responsive microRNAs and their role in the environmental stress survival of tolerant animals

Biggar

Storey

2015

Journal of Experimental Biology

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Living animals are constantly faced with various environmental stresses that challenge normal life, including: oxygen limitation, very low or high temperature, as well as restriction of water and food. It has been well established that in response to these stresses, tolerant organisms regularly respond with a distinct suite of cellular modifications that involve transcriptional, translational and posttranslational modification. In recent years, a new mechanism of rapid and reversible transcriptome regulation, via the action of noncoding RNA molecules, has emerged into post-transcriptional regulation and has since been shown to be part of the survival response. However, these RNA-based mechanisms by which tolerant organisms respond to stressed conditions are not well understood. Recent studies have begun to show that non-coding RNAs control gene expression and translation of mRNA to protein, and can also have regulatory influence over major cellular processes. For example, select microRNAs have been shown to have regulatory influence over the cell cycle, apoptosis, signal transduction, muscle atrophy and fatty acid metabolism during periods of environmental stress. As we are on the verge of dissecting the roles of non-coding RNA in environmental stress adaptation, this Commentary summarizes the hallmark alterations in microRNA expression that facilitate stress survival.

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The translation state of differentially expressed mRNAs in the hibernating 13-lined ground squirrel (Spermophilus tridecemlineatus)

Cited by 85 publications

References 24 publications

Partial Gene Deletion of Heart-Type Fatty Acid–Binding Protein Limits the Severity of Dietary-Induced Insulin Resistance

Partial Gene Deletion of Heart-Type Fatty Acid–Binding Protein Limits the Severity of Dietary-Induced Insulin Resistance

Mammalian hibernation: differential gene expression and novel application of epigenetic controls

Insight into post-transcriptional gene regulation: stress-responsive microRNAs and their role in the environmental stress survival of tolerant animals

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