A simplified PCR test for early detection of dwarf off-types in micropropagated Cavendish bananas (Musa spp. AAA)

Ramage, C.; Borda, Ana Maria; Hamill, S. D.; Smith, Malcolm W.

doi:10.1016/j.scienta.2004.04.015

Cited by 31 publications

(22 citation statements)

References 7 publications

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“…This might be due to the reason that the present variation obtained during multiplication and rooting stages might be due to genetic variations which are caused by changes in DNA nucleotide sequence. These findings are in agreement with the reports of Ramage et al, (2004) who reported that some dwarf somaclonal variants in in vitro cultures of banana var. Williams (Cavendish banana) were relatively stable and did not generally revert to a normal phenotype with time.…”

Section: Phenotypic Appearance Of Plantlets At the End Of Rooting Stasupporting

confidence: 94%

Analysis of the Genetic Stability of Banana Tissue Culture Propagated Plantlets cv. Ney Poovan (AB) Using Morphological and Molecular Markers

Bhalang¹,

Prabhuling²,

Hipparagi³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Section: Phenotypic Appearance Of Plantlets At the End Of Rooting Stasupporting

confidence: 94%

Analysis of the Genetic Stability of Banana Tissue Culture Propagated Plantlets cv. Ney Poovan (AB) Using Morphological and Molecular Markers

Bhalang¹,

Prabhuling²,

Hipparagi³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…Williams (Cavendish banana), have been reported (Israeli et al 1996) and such dwarf somaclones were relatively stable and did not generally revert to a normal phenotype (Ramage et al 2004). It has been reported in Musa that the extent of instability caused by in vitro culture was related to cultivar rather than the culture condition (Ray et al 2006).…”

Section: Resultsmentioning

confidence: 99%

“…ISSR technique is also very simple, fast, cost-effective, highly discriminative and reliable (Pradeep et al 2002). At present, RAPD and ISSR markers have been successfully applied to detect the genetic similarities or dissimilarities in micropropagated material in various plants (Carvalho et al 2004;Martins et al 2004;Ramage et al 2004;Modgil et al 2005). The study presented here was undertaken to monitor the genetic stability of long term micropropagated shoots of a banana clone using the RAPD and ISSR technique.…”

mentioning

confidence: 99%

ISSR (inter-simple sequence repeats)

2008

Encyclopedia of Genetics, Genomics, Proteomics and Informatics

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A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cultures with distinct variation in morphological characteristics (morphotypes). Similarly, the mother maintained in the field was also subjected for genetic analysis. Out of the 50 RAPD and 25 ISSR primers screened, 30 RAPD and 5 ISSR primers produced totally 424 clear, distinct and reproducible band classes resulting in a total of 5088 bands where the banding patterns for each primer was highly uniform and comparable to the field-grown *Corresponding author mother clone from which the cultures had been established. These results indicate that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and applicable for clonal propagation of banana var. NR over a long period. This is the first report on the use of genetic markers to establish genetic fidelity of long-term micropropagated banana using RAPD and ISSR.

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“…These primers are derived from the conversion of other markers, many of them from random amplified polymorphic DNA (RAPD) markers. Several studies in the literature have used different approaches with SCAR markers in bananas (Javed et al, 2004;Ramage et al, 2004;Suprassana et al, 2008;Nwauzoma and Saraswath;Wang et al, 2012). The research of Wang et al (2012) was used as the basis for the present study.…”

Section: Introductionmentioning

confidence: 99%

Development of a thematic collection of Musa spp accessions using SCAR markers for preventive breeding against Fusarium oxysporum f. sp cubense tropical race 4

et al. 2016

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ABSTRACT. Bananas are one of the most consumed fruits worldwide, but are affected by many pests and diseases. One of the most devastating diseases is Fusarium wilt, caused by Fusarium oxysporum f. sp cubense (Foc). Recently, Fusarium tropical race 4 (Foc TR4) has been causing irreparable damage, especially in Asia and Africa where it has devastated entire plantations, including areas with Cavendish, which is known to be resistant to Foc race 1. Although this race is not yet present in Brazil, results obtained by Embrapa in partnership with the University of Wageningen, The Netherlands, indicate that 100% of the cultivars used by Brazilian growers are susceptible to Foc TR 4. In our study, 276 banana accessions were screened with sequence characterized amplified region (SCAR) markers that have been linked to the resistance of Foc TR 4. Two SCAR primers were tested and the results revealed that SCAR ScaU1001 was efficient at discriminating accessions with possible resistance in 36.6% of the evaluated accessions. This is the first attempt to develop a thematic collection of possible Foc TR 4 resistant banana accessions in Brazil, which could be tested in Asian or African countries to validate markerassisted selection (MAS), and for use in the preventive breeding of the crop to safeguard our banana plantations against Foc TR 4. We believe that this is an important step towards the prevention of this devastating disease, especially considering that our banana plantations are at risk.

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A simplified PCR test for early detection of dwarf off-types in micropropagated Cavendish bananas (Musa spp. AAA)

Cited by 31 publications

References 7 publications

Analysis of the Genetic Stability of Banana Tissue Culture Propagated Plantlets cv. Ney Poovan (AB) Using Morphological and Molecular Markers

Analysis of the Genetic Stability of Banana Tissue Culture Propagated Plantlets cv. Ney Poovan (AB) Using Morphological and Molecular Markers

ISSR (inter-simple sequence repeats)

Development of a thematic collection of Musa spp accessions using SCAR markers for preventive breeding against Fusarium oxysporum f. sp cubense tropical race 4

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