Platelet-rich plasma for osteoarthritis treatment

Knop, E; Paula, Luiz Eduardo de; Fuller, Ricardo

doi:10.1016/j.rbre.2015.07.002

Cited by 31 publications

(38 citation statements)

References 40 publications

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“…Recent studies show high variability in the levels of GFs and inflammatory mediators (IMs) between males and females, between different individuals, and between samples from the same individual depending on timing and method of preparation . The multiple sources of variability in PRP composition substantially complicate understanding mechanisms of action and evaluation of clinical effectiveness …”

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confidence: 99%

Platelet‐Rich Plasma (PRP) From Older Males With Knee Osteoarthritis Depresses Chondrocyte Metabolism and Upregulates Inflammation

O’Donnell

Migliore

Grandi

et al. 2019

Journal Orthopaedic Research

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There is intense clinical interest in the potential effects of platelet-rich plasma (PRP) for the treatment of osteoarthritis (OA). This study tested the hypotheses that (i) "lower" levels of the inflammatory mediators (IMs), interleukin-1β, and tumor necrosis factor α (TNF-α) and (ii) "higher" levels of the growth factors (GFs), insulin-like growth factor 1, and transforming growth factor β1 within leukocyte-poor PRP correlate with more favorable chondrocyte and macrophage responses in vitro. Samples were collected from 10 "healthy" young male (23-33 years old) human subjects (H-PRP) and nine older (62-85 years old) male patients with severe knee OA (OA-PRP). The samples were separated into groups of "high" or "low" levels of IM and GF based on multiplex cytokine and enzyme-linked immunosorbent assay data. Three-dimensional (3D) alginate bead chondrocyte cultures and monocyte-derived macrophage cultures were treated with 10% PRP from donors in different groups. Gene expression was analyzed by quantitative polymerase chain reaction. Contrary to our hypotheses, the effect of PRP on chondrocytes and macrophages was mainly influenced by the age and disease status of the PRP donor as opposed to the IM or GF groupings. While H-PRP showed similar effects on expression of chondrogenic markers (Col2a1 and Sox9) as the negative control group (p > 0.05), OA-PRP decreased chondrocyte expression of Col2a1 and Sox-9 messenger RNA by 40% and 30%, respectively (Col2a1, p = 0.015; Sox9, p = 0.037). OA-PRP also upregulated TNF-α and matrix metallopeptidase 9 (p < 0.001) gene expression in macrophages while H-PRP did not. This data suggests that PRP from older individuals with OA contain factors that may suppress chondrocyte matrix synthesis and promote macrophage inflammation in vitro.

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confidence: 99%

Platelet‐Rich Plasma (PRP) From Older Males With Knee Osteoarthritis Depresses Chondrocyte Metabolism and Upregulates Inflammation

O’Donnell

Migliore

Grandi

et al. 2019

Journal Orthopaedic Research

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“…Ketika PRP disuntikkan ke tempat yang terluka, trombosit diaktifkan oleh trombin endogen atau kolagen intraartikular. Setelah diaktifkan akan timbul sekresi growth factor dan mediator mediator inflamasi seperti platelet derived growth factor (PDGF), antagonis reseptor interleukin-1 (IL-1RA), soluble receptor of tumor necrosis factor (TNF-RI), tumor growth factor þ (TGF-þ), platelet factor 4 (PF4) , vascular endotel growth factor (VEGF), epidermal growth factor (EGF), insulin growth factor (IGF), osteocalcin (Oc), osteonektin, fibrinogen, vitroneektin, bronkektin dan trombospondin (Knop, Paula, & Fuller, 2015).…”

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“…Faktor-faktor lain seperti IGF-1, PDGF dan TGF-favor mendukung stabilisasi tulang rawan dengan mengendalikan fungsi metabolisme kondrosit dan tulang subkondral, mempertahankan homeostasis antara sintesis dan degradasi proteoglikan, dan merangsang proliferasi kondrosit. platelet growth factor juga dapat menstimulasi fibroblast sinovial untuk mensintesis asam hialuronat (Knop, et al, 2015).…”

Section: Pembahasanunclassified

Literature Review: Perbandingan Efektivitas Platelet Rich Plasma (PRP) dengan Asam Hialuronat (HA) sebagai Terapi Osteoarthritis

Anzani

2020

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The prevalence of osteoarthritis is increasing with age. In osteoarthritis, there is a change in the joint process that fails to repair damaged cartilages and manifestations such as joint narrowing, osteophytosis, subcondral sclerosis, and abnormal bone shape. Pharmacological therapies such as analgesics, corticosteroids, glucosamine, chondroitin sulfate, and non-steroidal anti-inflammatory drugs are used together with viscosupplementation to help and improve the development of arthritis. Intra-articular injection therapy is also used as a good choice for osteoarthritis therapy. Hyaluronic acid (HA) and platelet-rich plasma (PRP) are the two choices of intraarticular therapy that can be used. Hyaluronic acid can reshape cartilage through aggregation with proteoglycans and can improve synovial fluid present in the joints, but therapy with hyaluronic acid has a long-lasting, expensive, and proven effect. Platelet-rich plasma (PRP) is a new therapy. PRP is obtained from blood centrifugation several times. PRP is very easy to get, cheap and non-invasive. PRP contains various growth factors that can regenerate damaged cartilages. PRP can be applied and has the same benefits as HA, but until now there has been no consensus that which is a better outcome between the two therapies. From various literature it is known that PRP is more effective than hyaluronic acid as osteoarthritis therapy.

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“…However, there are several PRP preparations with different cellular (platelets and white blood cells (WBCs)) and molecular (growth factors (GFs) and cytokines) profiles, which undoubtedly produce different joint tissue responses after they come into contact with these substances [1, 2, 7, 8]. …”

Section: Introductionmentioning

confidence: 99%

Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide

Carmona

Ríos

López

et al. 2017

Veterinary Medicine International

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Platelet-rich plasma (PRP) preparations are used in horses with osteoarthritis (OA). However, some controversies remain regarding the ideal concentration of platelets and leukocytes to produce an adequate anti-inflammatory and anabolic response in the synovial membrane. The aims of this study were to study the influence of leukoconcentrated platelet-rich gel (Lc-PRG) and leukoreduced platelet-rich gel (Lr-PRG) supernatants on the quantitative expression of some proinflammatory and anabolic genes in equine synovial membrane explants (SMEs) challenged with lipopolysaccharide (LPS). SMEs from six horses were cultured over 96 h. Then, SMEs were harvested for RNA extraction and quantitative gene expression analysis by RT-qPCR for nuclear factor kappa B (NFκB), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), collagen type II alpha 1 (COL2A1), and cartilage oligomeric matrix protein (COMP). The 25% and 50% Lc-PRG supernatants led to downregulation of NFκB, MMP-13, ADAMTS-4, COL1A1, COL2A1, and COMP in SMEs. Lr-PRG supernatants (particularly at the 50% concentration) induced downregulation of NFκB, MMP-13, ADAMTS-4, and COL1A1 and upregulation of COL2A1 and COMP. Lr-PRG supernatants should be used for the treatment of inflammatory arthropathies in horses because they have anti-inflammatory and anabolic effects in the synovial membrane.

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Platelet-rich plasma for osteoarthritis treatment

Cited by 31 publications

References 40 publications

Platelet‐Rich Plasma (PRP) From Older Males With Knee Osteoarthritis Depresses Chondrocyte Metabolism and Upregulates Inflammation

Platelet‐Rich Plasma (PRP) From Older Males With Knee Osteoarthritis Depresses Chondrocyte Metabolism and Upregulates Inflammation

Literature Review: Perbandingan Efektivitas Platelet Rich Plasma (PRP) dengan Asam Hialuronat (HA) sebagai Terapi Osteoarthritis

Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide

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