Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membrane

Souza, Fernanda Gimenez de; Fernandes, Beatriz Luci; Rebelatto, Cármen Lúcia Kuniyoshi; Aguiar, Alessandra Melo de; Fracaro, Letícia; Brofman, Paulo Roberto Slud

doi:10.1016/j.rboe.2017.12.004

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…Reports on chondro differentiation-promoting effects of platelets aggregates, such as PRP, on MSCs are contentious. Several previously confirmed chondro-inductive stimuli of platelet concentrates to MSCs [38,[53][54][55], whereas others indicate that PRP treatment does not improve the in vitro chondrogenesis of MSCs [56]. The difference between the previously identified differentiation-promoting effects of platelets aggregates such as PRP in musculoskeletal diseases (reviewed by Qian et al [57]), and our data on L-PRF might be caused by different factors.…”

Section: Discussionsupporting

confidence: 53%

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Monaco

Gervois

Beaumont

et al. 2020

Cells

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Osteoarthritis (OA) is a degenerative and inflammatory joint disorder with cartilage loss. Dental pulp stem cells (DPSCs) can undergo chondrogenic differentiation and secrete growth factors associated with tissue repair and immunomodulation. Leukocyte-and platelet-rich fibrin (L-PRF) emerges in regenerative medicine because of its growth factor content and fibrin matrix. This study evaluates the therapeutic application of DPSCs and L-PRF in OA via immunomodulation and cartilage regeneration. Chondrogenic differentiation of DPSCs, with or without L-PRF exudate (ex) and conditioned medium (CM), and of bone marrow-mesenchymal stem cells was compared. These cells showed differential chondrogenesis. L-PRF was unable to increase cartilage-associated components. Immature murine articular chondrocytes (iMACs) were cultured with L-PRF ex, L-PRF CM, or DPSC CM. L-PRF CM had pro-survival and proliferative effects on unstimulated and cytokine-stimulated iMACs. L-PRF CM stimulated the release of IL-6 and PGE2, and increased MMP-13, TIMP-1 and IL-6 mRNA levels in cytokine-stimulated iMACs. DPSC CM increased the survival and proliferation of unstimulated iMACs. In cytokine-stimulated iMACs, DPSC CM increased TIMP-1 gene expression, whereas it inhibited nitrite release in 3D culture. We showed promising effects of DPSCs in an in vitro OA model, as they undergo chondrogenesis in vitro, stimulate the survival of chondrocytes and have immunomodulatory effects.

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Section: Discussionsupporting

confidence: 53%

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Monaco

Gervois

Beaumont

et al. 2020

Cells

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“…PRF facilitates the proliferation, migration, and differentiation of chondrocytes [62]. Souza et al demonstrated the proliferation and differentiation of adipose tissue-derived stem cells when combined with PRF membrane [63].…”

Section: Cartilage Regenerationmentioning

confidence: 99%

Evolution and Clinical Advances of Platelet-Rich Fibrin in Musculoskeletal Regeneration

Narayanaswamy¹,

Patro

Jeyaraman³

et al. 2023

Bioengineering

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Over the past few decades, various forms of platelet concentrates have evolved with significant clinical utility. The newer generation products, including leukocyte-platelet-rich fibrin (L-PRF) and advanced platelet-rich fibrin (A-PRF), have shown superior biological properties in musculoskeletal regeneration than the first-generation concentrates, such as platelet-rich plasma (PRP) and plasma rich in growth factors. These newer platelet concentrates have a complete matrix of physiological fibrin that acts as a scaffold with a three-dimensional (3D) architecture. Further, it facilitates intercellular signaling and migration, thereby promoting angiogenic, chondrogenic, and osteogenic activities. A-PRF with higher leukocyte inclusion possesses antimicrobial activity than the first generations. Due to the presence of enormous amounts of growth factors and anti-inflammatory cytokines that are released, A-PRF has the potential to replicate the various physiological and immunological factors of wound healing. In addition, there are more neutrophils, monocytes, and macrophages, all of which secrete essential chemotactic molecules. As a result, both L-PRF and A-PRF are used in the management of musculoskeletal conditions, such as chondral injuries, tendinopathies, tissue regeneration, and other sports-related injuries. In addition to this, its applications have been expanded to include the fields of reconstructive cosmetic surgery, wound healing in diabetic patients, and maxillofacial surgeries.

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Platelet-Rich Fibrin Scaffolds for Cartilage and Tendon Regenerative Medicine: From Bench to Bedside

Barbon

Stocco

Macchi

et al. 2019

IJMS

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Nowadays, research in Tissue Engineering and Regenerative Medicine is focusing on the identification of instructive scaffolds to address the requirements of both clinicians and patients to achieve prompt and adequate healing in case of injury. Among biomaterials, hemocomponents, and in particular Platelet-rich Fibrin matrices, have aroused widespread interest, acting as delivery platforms for growth factors, cytokines and immune/stem-like cells for immunomodulation; their autologous origin and ready availability are also noteworthy aspects, as safety- and cost-related factors and practical aspects make it possible to shorten surgical interventions. In fact, several authors have focused on the use of Platelet-rich Fibrin in cartilage and tendon tissue engineering, reporting an increasing number of in vitro, pre-clinical and clinical studies. This narrative review attempts to compare the relevant advances in the field, with particular reference being made to the regenerative role of platelet-derived growth factors, as well as the main pre-clinical and clinical research on Platelet-rich Fibrin in chondrogenesis and tenogenesis, thereby providing a basis for critical revision of the topic.

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Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membrane

Cited by 3 publications

References 23 publications

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Evolution and Clinical Advances of Platelet-Rich Fibrin in Musculoskeletal Regeneration

Platelet-Rich Fibrin Scaffolds for Cartilage and Tendon Regenerative Medicine: From Bench to Bedside

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