Profile of collagen gene expression in the glenohumeral capsule of patients with traumatic anterior instability of the shoulder

Belangero, Paulo Santoro; Leal, Mariana Ferreira; Pochini, Alberto de Castro; Andreoli, Carlos Vicente; Ejnisman, Benno; Cohen, Moisés

doi:10.1016/j.rboe.2014.10.008

Cited by 5 publications

(8 citation statements)

References 28 publications

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“…This heterogeneity may have also contributed to false negatives. Third, molecular alterations may occur in other capsule regions and may have a different etiological role in capsular injury 30 - 32 . We evaluated the AI region because this portion of the capsule presented macroscopic injuries (i.e., a high level of inflammation) during arthroscopic examination of the studied patients.…”

Section: Discussionmentioning

confidence: 99%

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The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study

Cohen¹,

Leal²,

Belangero³

et al. 2016

Clinics

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OBJECTIVES:We evaluated mRNA expression levels of genes that encode TGF-β1; the TGF-β1 receptor; the collagen-modifying enzymes LOX, PLOD1, and PLOD2; and the extracellular matrix proteins COMP, FN1, TNC and TNXB in synovial/capsule specimens from patients with idiopathic adhesive capsulitis. Possible associations between the measured mRNA levels and clinical parameters were also investigated.METHODS:We obtained glenohumeral joint synovium/capsule specimens from 9 patients with idiopathic adhesive capsulitis who had not shown improvement in symptoms after 5 months of physiotherapy. Adhesive capsulitis was confirmed in all patients by magnetic resonance imaging. We also obtained specimens from 8 control patients who had underwent surgery for acute acromioclavicular joint dislocation and who had radiological indication of glenohumeral capsule alteration based on arthroscopic evaluation. mRNA expression in the synovium/capsule specimens was analyzed by quantitative reverse transcription PCR. The B2M and HPRT1 genes were used as references to normalize target gene expression in the shoulder tissue samples.RESULTS:The synovium/capsule samples from the patients with adhesive capsulitis had significantly higher TNC and FN1 expression than those from the controls. Additionally, symptom duration directly correlated with expression of TGFβ1 receptor I.CONCLUSION:Elevated levels of TNC and FN1 expression may be a marker of capsule injury. Upregulation of TGFβ1 receptor I seems to be dependent on symptom duration; therefore, TGFβ signaling may be involved in adhesive capsulitis. As such, TNC, FN1 and TGFβ1 receptor I may also play roles in adhesive capsulitis by contributing to capsule inflammation and fibrosis.

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Section: Discussionmentioning

confidence: 99%

“…To reduce sampling variation, only two of the authors (CC and BE) were responsible for collecting the tissue samples. The samples were collected as previously described 30 - 32 . As the synovium is adhered to the capsule, it cannot be separated from the capsule using arthroscopic instruments.…”

Section: Methodsmentioning

confidence: 99%

The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study

Cohen¹,

Leal²,

Belangero³

et al. 2016

Clinics

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“… 8 , 14 Even genetic studies, in the early stages, are being conducted in order to improve treatment outcomes. 15 …”

Section: Discussionmentioning

confidence: 99%

Analysis of postoperative monitoring of patients undergoing shoulder arthroscopy for anterior instability

Almeida¹,

Menegotto²,

Almeida³

et al. 2017

Revista Brasileira de Ortopedia (English Edition)

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ObjectiveAnalyze the postoperative follow-up of patients undergoing shoulder arthroscopy for treatment of anterior instability and correlate with the prevalence of recurrence.MethodsA six-question survey was applied by phone and mail to 65 patients, seeking information on the current result of the surgical procedure. All patients were treated arthroscopically for anterior shoulder instability, with at least 12 months of postoperative time. Patients with associated posterior labial lesions and revision surgeries were not included.ResultsAt the time of the survey the patients had a median of 56 (IQR: 34.5–110.5) postoperative months. The mean sample age was 24.6 years (maximum = 47, minimum = 12; SD = 7.3). Complaint of pain in the shoulder was observed in 20 patients (30.7%). Dislocation recurrence was observed in 10 patients (15.3%). Forty-four patients (67.6%) considered their shoulder normal, which was more frequent in non-recurrence patients (p < 0.001). Forty-three patients (66.1%) returned to their previous level of sport and there was no difference between recurrence and non-recurrence patients (p = 0.456). It was found that the prevalence of recurrence was 5.6 (95% CI: 1.30–24.46) times higher in individuals who abandoned monitoring before six months postoperatively (p = 0.012).ConclusionThe abandonment of postoperative monitoring in the early stages, when the patients receive orientation for muscle strengthening, proprioceptive education, and dangerous movements to avoid, can increase the rates of recurrent shoulder dislocation in patients treated for anterior instability by arthroscopy.

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“…Initially, Belangero et al 41 investigated the expression of the genes COL1A1, COL1A2, COL3A1 and COL5A1 in the anteroinferior region (macroscopically altered) and compared with the anterosuperior region of the glenohumeral capsule of 18 patients with traumatic anterior instability (TAI). They identified reduced expression of COL5A1 in the upper region.…”

Section: Shoulder Instabilitymentioning

confidence: 99%

Aspectos genéticos nas afecções do ombro

Cohen

Figueiredo

Belangero

et al. 2020

Rev Bras Ortop (Sao Paulo)

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ResumoA influência da herança genética tem sido cada vez mais investigada nas afecções do ombro, como a lesão do manguito rotador, instabilidade e ombro congelado. Ainda que os achados iniciais sejam pouco esclarecedores, é necessário construir progressivamente um banco de marcadores genéticos para catalogar perfis genômicos que, mais adiante, poderão contribuir para a previsão do risco da doença, desenvolvimento de melhores ferramentas de diagnóstico e tratamento. O presente artigo busca atualizar o que há de evidências de estudos genéticos na literatura para essas doenças, desde análises de polimorfismos, expressão de genes candidatos em tecidos e estudos de associação genômica ampla (GWAS, na sigla em inglês). Porém, é necessário apontar que existe grande dificuldade na replicação e utilização dos achados, principalmente em razão da falta de poder estatístico, da alta taxa de resultados falso-positivos e da grande quantidade de variáveis envolvidas.

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Profile of collagen gene expression in the glenohumeral capsule of patients with traumatic anterior instability of the shoulder

Cited by 5 publications

References 28 publications

The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study

The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study

Analysis of postoperative monitoring of patients undergoing shoulder arthroscopy for anterior instability

Aspectos genéticos nas afecções do ombro

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