Morphology and fine organization of the spermatheca of Haplotropis brunneriana (Orthoptera: Pamphagidae)

Li, Ké; Yang, Yujing; Mei, Lei; Zhang, Jinhui; Geng, Jianxun; Yin, Huan

doi:10.1016/j.rbe.2017.08.003

Cited by 3 publications

(1 citation statement)

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“…The results highlight the corrugated structure of the intima, and show that mating induces changes in the intima geometric properties. Interestingly, corrugated intima is a conserved structure also seen in other insects, such as, for example, the ductus receptaculi of Teleogryllus commodus (Orthoptera) 81 , the seminal receptacle of Armadillidium granulatum (Isopoda) 82 , and the spermatheca and seminal receptacle of Haplotropis brunneriana (Orthoptera) 83 . Visualization and segmentation analyses of FIB-SEM data showed EVs on the apical surface in virgin and mated distal seminal receptacles.…”

Section: Discussionmentioning

confidence: 91%

Three-dimensional correlative microscopy of the Drosophila female reproductive tract reveals modes of communication in seminal receptacle sperm storage

Zelinger,

Brumfeld,

Rechav

et al. 2024

Commun Biol

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In many taxa, females store sperm in specialized storage organs. Most insect sperm storage organs have a tubular structure, typically consisting of a central lumen surrounded by epithelial cells. These specialized tubules perform the essential tasks of transporting sperm through the female reproductive tract and supporting long-term sperm survival and function. Little is known about the way in which female sperm storage organs provide an environment conducive to sperm survival. We address this using a combined light microscopy, micro computed tomography (microCT), and Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) approach for high-resolution correlative three-dimensional imaging to advance our understanding of sperm-female interactions in Drosophila melanogaster. Using this multimodal approach, we were able to scan the lower female reproductive tract and distal portion of the seminal receptacle at low magnification, and to subsequently zoom in for further analysis on an ultrastructural level. Our findings highlight aspects of the way in which the seminal receptacle keeps sperm viable in the lumen, and set the stage for further studies. The methods developed are suitable not only for Drosophila but also for other organisms with soft, delicate tissues.

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Section: Discussionmentioning

confidence: 91%