Two new species of Mycodrosophila (Diptera, Drosophilidae) proposed by molecular and morphological approaches, with a key to American species

Junges, João; Gottschalk, Marco Silva; Loreto, Élgion Lúcio da Silva; Robe, Lizandra Jaqueline

doi:10.1016/j.rbe.2015.11.008

Cited by 9 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As emphasised by Hebert and Gregory (), DNA barcoding aids in delimiting species, but is never sufficient to describe new taxa; thus, traditional taxonomy will certainly remain fundamental in the corroboration and definitive identification of the putative new species. For this reason, some of the potentially new species described here are already being described by our research group (Junges et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Neotropical mycophagous drosophilids (Diptera: Drosophilidae): DNA barcoding as a way of overcoming the taxonomic impediment

Machado

Santos

Fonseca

et al. 2017

Insect Conserv Diversity

Self Cite

View full text Add to dashboard Cite

Within Drosophilidae, the Zygothrica genus group corresponds to a speciose mycophagous group, which remains insufficiently investigated despite its potential for ecological and evolutionary studies. The recognised gap in the knowledge of species diversity and the threat provided by global warming due to niche conservatism and stringent temperature and humidity requirements make this group an excellent candidate for testing the implementation of DNA barcoding, which is the aim of this study. Extensive prospecting in various parts of Brazil provided 220 specimens. Considering both the morphological and molecular results, these individuals consisted of 56 species that could be distributed into three categories: 22 corresponded to previously described species, 24 very likely corresponded to undescribed species, and 10 uncertain cases corresponded mainly to one or a few females. Intra‐specific distances ranged from 0% to 5.3%, whereas inter‐specific congeneric and inter‐generic distances ranged from 2.4% to 21.7% and from 7.4% to 20% respectively. Moreover, all species were revealed as reciprocally monophyletic and presented positive barcoding gap values; 62.5% of them presented diagnostic characters. Although coalescent‐based methodologies suggested some additional splitting, species initially considered to be different were never lumped together. Therefore, in general, DNA barcoding of mycophagous drosophilids provided results that are largely congruent throughout different molecular approaches and also with morphological boundaries. Several putative new species were revealed, highlighting the potential of cytochrome c oxidase subunit I (COI) barcodes as a tool complementary to traditional taxonomy in unravelling cryptic diversity among Neotropical mycophagous drosophilids.

show abstract

Section: Discussionmentioning

confidence: 97%

Neotropical mycophagous drosophilids (Diptera: Drosophilidae): DNA barcoding as a way of overcoming the taxonomic impediment

Machado

Santos

Fonseca

et al. 2017

Insect Conserv Diversity

Self Cite

View full text Add to dashboard Cite

show abstract

“…3, Favolus tenuiculus, Rigidoporus lineatus, Rigidoporus microporus and Trametes modesta), had a relative frequency of 60% and 86% of the collected fungi species were sampled in a single collection. Out of the nine Mycodrosophila species found in the Neotropics, only five had been previously recorded in Brazil (Val et al, 1981;Junges et al, 2016). In this study, five Mycodrosophila species were observed (Table 1), including the first records of M. neoprojectans and M. pseudoprojectans to Brazil.…”

Section: Resultsmentioning

confidence: 43%

“…ISSN On-Line: 1807-0205 ISSN Printed: 0031-1049 ISNI: 0000-0004- In the Neotropics, few studies have widely and systematically addressed Drosophilidae mycophagy. The taxonomy of this group, however, is quite well understood than other drosophilids (Sturtevant, 1920;Hendel, 1936;Frota-Pessoa, 1945, 1951Cordeiro, 1952;Burla, 1956;Brncic, 1957;Wheeler & Takada, 1963;Mourão et al, 1965Mourão et al, , 1967Grimaldi, 1987;Junges et al, 2016). The descriptions of Hirtodrosophila Duda, Mycodrosophila Oldenberg and Zygothrica Wiedemann species were, most of them from specimens in entomological collections.…”

Section: Introductionmentioning

confidence: 99%

Mycophagous Drosophilidae (Diptera) guild and their hosts in the Brazilian Amazon

Santa-Brígida

Wartchow

Medeiros³

et al. 2019

Pap. Avulsos Zool.

Self Cite

View full text Add to dashboard Cite

The composition and potential hosts of mycophagous Drosophilidae from a section of the Brazilian Amazon forest in the Caxiuanã National forest were investigated. Sampling was performed in three different periods at long the wet season (January (beginning) and July (end) 2013 and May (middle) 2014). The samples were collected from existing trails by actively searching for fungal fruiting bodies where Drosophilidae were present. We present composition and richness analysis over two years of sampling sampling Drosophilidae and Fungi. We evaluate sampling completeness using asymptotic species richness estimators. Out of 159 fruiting body samples and 64 fungal species, 5,124 drosophilids belonging to 55 species and 5 genera were collected. The mycophagous Drosophilidae richness values estimated by Jackknife 1 and Bootstrap were 69 and 61, respectively. The estimated fly richness correlated positively with fungal richness and abundance. Among the Drosophilidae species identified in this study, approximately 5% represent new occurrences for Brazil and 56% represent new species. Four genera belonging to the Zygothrica genus group are found in the Amazon region, and these genera represent 80% of the fungus-associated fauna known to date for the tropics. In conclusion, our results show that the fungal richness and abundance were the factors that determined the high diversity of mycophagous Drosophilidae.

show abstract

“…This group is composed by more than 437 species of the genera Hirtodrosophila Duda, 1923, Mycodrosophila Oldenberg, 1914, Paraliodrosophila Duda, 1925, Paramycodrosophila Duda, 1924, and Zygothrica Wiedemann, 1830 (Grimaldi 1990) that share some trophic associations with fungi, being generally referred as mycophagous species. These species are widespread around the globe, with many recent records for the Neotropics (Machado et al 2017;Junges et al 2016Junges et al , 2019Mendes and Gottschalk 2019). Although the Neotropical members of the group were recently shown to comprise a monophyletic lineage (Gautério et al 2020), this status does not seem to apply to the genus Hirtodrosophila (Gautério et al 2020) or even to the genus group considered as a whole (Russo et al 2013;Yassin 2013).…”

Section: Introductionmentioning

confidence: 99%

Comparative mitogenomics of Drosophilidae and the evolution of the Zygothrica genus group (Diptera, Drosophilidae)

et al. 2021

Self Cite

View full text Add to dashboard Cite

The Zygothrica genus group of Drosophilidae encompasses more than 437 species and five genera. Although knowledge regarding its diversity has increased, uncertainties about its monophyly and position within Drosophilidae remain. Genomic approaches have been widely used to address different phylogenetic questions and analyses involving the mitogenome have revealed a cost-efficient tool to these studies. Thus, this work aims to characterize mitogenomes of three species of the Zygothrica genus group (from the Hirtodrosophila, Paraliodrosophila and Zygothrica genera), while comparing them with orthologous sequences from other 23 Drosophilidae species and addressing their phylogenetic position. General content concerning gene order and overlap, nucleotide composition, start and stop codon, codon usage and tRNA structures were compared, and phylogenetic trees were constructed under different datasets. The complete mitogenomes characterized for H. subflavohalterata affinis H002 and P. antennta present the PanCrustacea gene order with 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, 13 protein coding genes and an A+T rich region with two T-stretched elements. Some peculiarities such as the almost complete overlap of genes tRNAH/ND4, tRNAF/ND5 and tRNAS2/ND1 are reported for different Drosophilidae species. Non-canonical secondary structures were encountered for tRNAS1 and tRNAY, revealing patterns that apply at different phylogenetic scales. According to the best depiction of the mitogenomes evolutionary history, the three Neotropical species of the Zygothrica genus group encompass a monophyletic lineage sister to Zaprionus, composing with this genus a clade that is sister to the Drosophila subgenus.

show abstract

Two new species of Mycodrosophila (Diptera, Drosophilidae) proposed by molecular and morphological approaches, with a key to American species

Cited by 9 publications

References 17 publications

Neotropical mycophagous drosophilids (Diptera: Drosophilidae): DNA barcoding as a way of overcoming the taxonomic impediment

Neotropical mycophagous drosophilids (Diptera: Drosophilidae): DNA barcoding as a way of overcoming the taxonomic impediment

Mycophagous Drosophilidae (Diptera) guild and their hosts in the Brazilian Amazon

Comparative mitogenomics of Drosophilidae and the evolution of the Zygothrica genus group (Diptera, Drosophilidae)

Contact Info

Product

Resources

About