Characterization of a mesophilic actinobacteria that degrades poly(butylene adipate-co-terephthalate)

Soulenthone, Phouvilay; Tachibana, Yuya; Muroi, Fumihiro; Suzuki, Miwa; Ishii, Naomi; Ohta, Yutaka; Kasuya, Ken-ichi

doi:10.1016/j.polymdegradstab.2020.109335

Cited by 27 publications

(5 citation statements)

References 44 publications

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“…Saccharothrix wayandensis, etc.) have shown either weight reduction or partial degradation of PE films (Sivan et al, 2006;Abraham et al, 2017;Huang et al, 2019;Soulenthone et al, 2020). Among the fungal species, members of Aspergillus (Muhonja et al, 2018); Fusarium (Sivan, 2011); Penicillium, Zalerion (Raddadi and Fava, 2019); Chaetomium and Pullularia (Sudhakar et al, 2008) are well known for biodegradation of LDPEs and HDPEs.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

et al. 2020

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Exploring the catabolic repertoire of natural bacteria for biodegradation of plastics is one of the priority areas of biotechnology research. Low Density Polyethylene (LDPE) is recalcitrant and poses serious threats to our environment. The present study explored the LDPE biodegradation potential of aerobic bacteria enriched from municipal waste dumpsite and bentonite based drilling fluids from a deep subsurface drilling operation. Considerable bacterial growth coupled with significant weight loss of the LDPE beads (∼8%), change in pH to acidic condition and biofilm cell growth around the beads (CFU count 105–106/cm2) were noted for two samples (P and DF2). The enriched microbial consortia thus obtained displayed high (65–90%) cell surface hydrophobicity, confirming their potential toward LDPE adhesion as well as biofilm formation. Two LDPE degrading bacterial strains affiliated to Stenotrophomonas sp. and Achromobacter sp. were isolated as pure culture from P and DF2 enrichments. 16S rRNA gene sequences of these isolates indicated their taxonomic novelty. Further biodegradation studies provided strong evidence toward the LDPE metabolizing ability of these two organisms. Atomic Fore Microscopy (AFM) and Scanning Electron Microscopy (SEM) revealed considerable damage (in terms of formation of cracks, grooves, etc.) on the micrometric surface of the LDPE film. Analysis of the average roughness (Ra), root mean square roughness (Rq), average height (Rz), maximum peak height (Rp), and maximum valley depth (Rv) (nano-roughness parameters) through AFM indicated 2–3 fold increase in nano-roughness of the LDPE film. FTIR analysis suggested incorporation of alkoxy (1000–1090 cm–1), acyl (1220 cm–1), nitro (1500–1600 cm–1), carbonyl (1720 cm–1) groups into the carbon backbone, formation of N-O stretching (1360 cm–1) and chain scission (905 cm–1) in the microbially treated LDPEs. Increase in carbonyl index (15–20 fold), double bond index (1.5–2 fold) and terminal double bond index (30–40 fold) confirmed that biodegraded LDPEs had undergone oxidation, vinylene formation and chain scission. The data suggested that oxidation and dehydrogenation could be the key steps allowing formation of low molecular weight products suitable for their further mineralization by the test bacteria. The study highlighted LDPE degrading ability of natural bacteria and provided the opportunity for their development in plastic remediation process.

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Section: Introductionmentioning

confidence: 99%

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

et al. 2020

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“…The low values of enzymatic activity reported for the actinobacteria Rhodococcus fascians in comparison to a mesophilic PBAT-degrading fungus show that both the type of enzyme and the microorganism producing the enzyme play major main roles in activity [ 267 , 268 ]. These results could be associated with the favorable conditions offered to microorganism populations in soil environments in the mesophilic range.…”

Section: Polymers Susceptible To Biodegradationmentioning

confidence: 99%

Biodegradation of Biodegradable Polymers in Mesophilic Aerobic Environments

Bher

Mayekar

Auras

et al. 2022

IJMS

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Finding alternatives to diminish plastic pollution has become one of the main challenges of modern life. A few alternatives have gained potential for a shift toward a more circular and sustainable relationship with plastics. Biodegradable polymers derived from bio- and fossil-based sources have emerged as one feasible alternative to overcome inconveniences associated with the use and disposal of non-biodegradable polymers. The biodegradation process depends on the environment’s factors, microorganisms and associated enzymes, and the polymer properties, resulting in a plethora of parameters that create a complex process whereby biodegradation times and rates can vary immensely. This review aims to provide a background and a comprehensive, systematic, and critical overview of this complex process with a special focus on the mesophilic range. Activity toward depolymerization by extracellular enzymes, biofilm effect on the dynamic of the degradation process, CO2 evolution evaluating the extent of biodegradation, and metabolic pathways are discussed. Remarks and perspectives for potential future research are provided with a focus on the current knowledge gaps if the goal is to minimize the persistence of plastics across environments. Innovative approaches such as the addition of specific compounds to trigger depolymerization under particular conditions, biostimulation, bioaugmentation, and the addition of natural and/or modified enzymes are state-of-the-art methods that need faster development. Furthermore, methods must be connected to standards and techniques that fully track the biodegradation process. More transdisciplinary research within areas of polymer chemistry/processing and microbiology/biochemistry is needed.

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“…If the thermophilic bacteria (typically belonging to actinomycetes strain) are able to rapidly cleavage the ester bonds at relatively high temperatures (50-60°C), at mild temperatures (25-30°C) the PBAT degradation, operated by fungal strains or mesophilic bacteria such as firmicutes and proteobacteria, is very slow. [60][61][62] The presence of fillers can be an interesting way to accelerate the biodegradation rate of PBAT. Indeed, aerobic bacteria, which act in the degradation of fillers in soil (controlled compost according to Standard ASTM D5988-12), are hydrophilic.…”

Section: Compostabilitymentioning

confidence: 99%

“…If the thermophilic bacteria (typically belonging to actinomycetes strain) are able to rapidly cleavage the ester bonds at relatively high temperatures (50–60 °C), at mild temperatures (25–30 °C) the PBAT degradation, operated by fungal strains or mesophilic bacteria such as firmicutes and proteobacteria, is very slow. [ 60 , 61 , 62 ]…”

Section: Composting Versus Re/upcycling For Specific Biodegradable Polymers: State Of the Artmentioning

confidence: 99%

End of Life of Biodegradable Plastics: Composting versus Re/Upcycling

et al. 2021

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Nowadays the issues related to the end of life of traditional plastics are very urgent due to the important pollution problems that plastics have caused. Biodegradable plastics can help to try to mitigate these problems, but even bioplastics need much attention to carefully evaluate the different options for plastic waste disposal. In this Minireview, three different end-of-life scenarios (composting, recycling, and upcycling) were evaluated in terms of literature review. As a result, the ability of bioplastics to be biodegraded by composting has been related to physical variables and materials characteristics. Hence, it is possible to deduce that the process is mature enough to be a good way to minimize bioplastic waste and valorize it for the production of a fertilizer. Recycling and upcycling options, which could open up many interesting new scenarios for the production of high-value materials, are less studied. Research in this area can be strongly encouraged.

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Characterization of a mesophilic actinobacteria that degrades poly(butylene adipate-co-terephthalate)

Cited by 27 publications

References 44 publications

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

Biodegradation of Biodegradable Polymers in Mesophilic Aerobic Environments

End of Life of Biodegradable Plastics: Composting versus Re/Upcycling

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