Morphological, transcriptomics and phytohormone analysis shed light on the development of a novel dwarf mutant of cabbage (Brassica oleracea)

Xing, Miaomiao; Su, Henan; Liu, Xing; Yang, Limei; Zhang, Yangyong; Wang, Yong; Fang, Zhiyuan; Lv, Honghao

doi:10.1016/j.plantsci.2019.110283

Cited by 21 publications

(5 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was illustrated that high concentrations of ABA not only inhibit cell division in the apical meristems but also repress the elongation of roots ( Bai et al, 2009 ; Takatsuka and Umeda, 2014 ). Expression of key genes related to ABA was significantly upregulated in cabbage dwarf mutant ( Xing et al, 2020 ). Controversially, the higher IAA but lower ABA contents in dwarf autotetraploid Chinese Cabbage was found ( Wang Y. et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…It should be noted that the factors affecting plant height are complex and diverse. Noticeably, Xing et al (2020) implied that the change in phytohormones is due to but not the cause of the dwarf trait in polyploidy cabbage. Further studies of transcriptome analysis are worth pursuing to facilitate a better understanding of dwarfism mechanism in tetraploid garlic.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro Induction and Phenotypic Variations of Autotetraploid Garlic (Allium sativum L.) With Dwarfism

et al. 2022

View full text Add to dashboard Cite

Garlic (Allium sativum L.) is a compelling horticultural crop with high culinary and therapeutic values. Commercial garlic varieties are male-sterile and propagated asexually from individual cloves or bulbils. Consequently, its main breeding strategy has been confined to the time-consuming and inefficient selection approach from the existing germplasm. Polyploidy, meanwhile, plays a prominent role in conferring plants various changes in morphological, physiological, and ecological properties. Artificial polyploidy induction has gained pivotal attention to generate new genotype for further crop improvement as a mutational breeding method. In our study, efficient and reliable in vitro induction protocols of autotetraploid garlic were established by applying different antimitotic agents based on high-frequency direct shoot organogenesis initiated from inflorescence explant. The explants were cultured on solid medium containing various concentrations of colchicine or oryzalin for different duration days. Afterward, the ploidy levels of regenerated plantlets with stable and distinguished characters were confirmed by flow cytometry and chromosome counting. The colchicine concentration at 0.2% (w/v) combined with culture duration for 20 days was most efficient (the autotetraploid induction rate was 21.8%) compared to the induction rate of 4.3% using oryzalin at 60 μmol L–1 for 20 days. No polymorphic bands were detected by simple sequence repeat analysis between tetraploid and diploid plantlets. The tetraploids exhibited a stable and remarkable dwarfness effect rarely reported in artificial polyploidization among wide range of phenotypic variations. There are both morphological and cytological changes including extremely reduced plant height, thickening and broadening of leaves, disappearance of pseudostem, density reduction, and augmented width of stomatal. Furthermore, the level of phytohormones, including, indole propionic acid, gibberellin, brassinolide, zeatin, dihydrozeatin, and methyl jasmonate, was significantly lower in tetraploids than those in diploid controls, except indole acetic acid and abscisic acid, which could partly explain the dwarfness in hormonal regulation aspect. Moreover, as the typical secondary metabolites of garlic, organosulfur compounds including allicin, diallyl disulfide, and diallyl trisulfide accumulated a higher content significantly in tetraploids. The obtained dwarf genotype of autotetraploid garlic could bring new perspectives for the artificial polyploids breeding and be implemented as a new germplasm to facilitate investigation into whole-genome doubling consequences.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In vitro Induction and Phenotypic Variations of Autotetraploid Garlic (Allium sativum L.) With Dwarfism

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The effects of exogenous hormones on plant height are mostly measured as the difference between the treated plant height and that of the control. Brassinosteroid, GA3 and IAA are closely related to plant dwarfism (Shahnejat-Bushehri et al, 2016;Xing et al, 2020). In this study, the promotion of plant height increase in mutant plants sprayed with different concentrations of IAA throughout the growth period was nonsignificant, nor was the plant height significantly different from the plants in the mutant D-CK group; however, heights of the IAAtreated plants were significantly lower than those of the W-CK plants (Table 1).…”

Section: Sophora Davidii Dwarf Mutant Plant Height Sensitivity To Dif...mentioning

confidence: 49%

Exogenous hormone application regulates dwarf mutant plant height in Sophora davidii (Franch.) Skeels by changing endogenous hormone levels

Xie

Sun

Zhao

et al. 2023

Chil. j. agric. res.

View full text Add to dashboard Cite

Dwarf mutants are valuable materials for investigating plant gene function and developing new crop varieties. In the present study, dwarf mutant (D-CK) and wild-type (W-CK) Sophora davidii (Franch.) Skeels plants foliarly sprayed with distilled water were used as controls, and dwarf mutants were treated with different concentrations of gibberellin A3 (GA3; 20, 40 and 60 mg L -1 ), brassinosteroid (BR; 12.5, 25 and 50 mg L -1 ); and auxin indole-3-acetic acid (IAA; 12.5, 25 and 50 mg L -1 ) to study the changes in plant height at 20, 40 and 60 d after exogenous hormone spraying. The changes in endogenous contents of IAA, GA3, BR, abscisic acid (ABA), jasmonic acid (JA), and salicylic acid (SA) and IAA:ABA ratio in the dwarf mutant plants were also measured at 20, 40 and 60 d after exogenous hormone application. The dwarf mutants reverted to the W-CK height phenotype after 60 d of exogenous spraying with different concentrations of GA3 and BR (except 50 mg•L -1 ), but after exogenous spraying with IAA, dwarf mutants did not revert to the W-CK height phenotype. Compared with D-CK plants, endogenous GA3, BR and IAA contents in dwarf mutant plants increased to some extent with exogenous spraying of GA3, BR and IAA. The results indicated that exogenous spraying of different concentrations of GA3 and BR regulated S. davidii plant height by promoting the secretion of endogenous GA3, BR, IAA and SA and inhibiting endogenous ABA secretion in dwarf mutant. The height of mutants was sensitive to exogenous GA3 and BR but not to exogenous IAA.

show abstract

“…Several hormones can cause plant dwarfing 31 . The concentrations of GA and BR are often considered to be the main cause of dwarf plants 48 . To better understand the involvement of phytohormone in dwarf mutant, we measured the contents of four major endogenous hormones in leaves of the mutant and the WT plants.…”

Section: Resultsmentioning

confidence: 99%

Analysis of a radiation-induced dwarf mutant of a warm-season turf grass reveals potential mechanisms involved in the dwarfing mutant

Lin

Ren

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Zoysia matrella [L.] Merr. is a widely cultivated warm-season turf grass in subtropical and tropical areas. Dwarf varieties of Z. matrella are attractive to growers because they often reduce lawn mowing frequencies. In this study, we describe a dwarf mutant of Z. matrella induced from the 60Co-γ-irradiated calluses. We conducted morphological test and physiological, biochemical and transcriptional analyses to reveal the dwarfing mechanism in the mutant. Phenotypically, the dwarf mutant showed shorter stems, wider leaves, lower canopy height, and a darker green color than the wild type (WT) control under the greenhouse conditions. Physiologically, we found that the phenotypic changes of the dwarf mutant were associated with the physiological responses in catalase, guaiacol peroxidase, superoxide dismutase, soluble protein, lignin, chlorophyll, and electric conductivity. Of the four endogenous hormones measured in leaves, both indole-3-acetic acid and abscisic acid contents were decreased in the mutant, whereas the contents of gibberellin and brassinosteroid showed no difference between the mutant and the WT control. A transcriptomic comparison between the dwarf mutant and the WT leaves revealed 360 differentially-expressed genes (DEGs), including 62 up-regulated and 298 down-regulated unigenes. The major DEGs related to auxin transportation (e.g., PIN-FORMED1) and cell wall development (i.e., CELLULOSE SYNTHASE1) and expansin homologous genes were all down-regulated, indicating their potential contribution to the phenotypic changes observed in the dwarf mutant. Overall, the results provide information to facilitate a better understanding of the dwarfing mechanism in grasses at physiological and transcript levels. In addition, the results suggest that manipulation of auxin biosynthetic pathway genes can be an effective approach for dwarfing breeding of turf grasses.

show abstract

Morphological, transcriptomics and phytohormone analysis shed light on the development of a novel dwarf mutant of cabbage (Brassica oleracea)

Cited by 21 publications

References 60 publications

In vitro Induction and Phenotypic Variations of Autotetraploid Garlic (Allium sativum L.) With Dwarfism

In vitro Induction and Phenotypic Variations of Autotetraploid Garlic (Allium sativum L.) With Dwarfism

Exogenous hormone application regulates dwarf mutant plant height in Sophora davidii (Franch.) Skeels by changing endogenous hormone levels

Analysis of a radiation-induced dwarf mutant of a warm-season turf grass reveals potential mechanisms involved in the dwarfing mutant

Contact Info

Product

Resources

About