Tracking of secretory phospholipase A2 enzyme activity levels from childhood to adulthood: a 21-year cohort

Chung, Olivia; Juonala, Markus; Mallat, Ziad; Hutri‐Kähönen, Nina; Viikari, Jorma; Raitakari, Olli T.; Magnussen, Costan G.

doi:10.1016/j.jped.2018.01.002

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…We used the same commercial assay and testing protocol to estimate sPLA2 activity as described by Maduwage et al 4 Notwithstanding, our findings only partly agree with the earlier observations. The key differences are as follows: first, the median sPLA2 activity level of 6.0 μmol/minute/mL in non-envenomed patients as reported by Maduwage et al 4 was 1000-fold higher than the circulating sPLA2 activity reported in healthy individuals and those with acute coronary syndromes, 7,8 in whom the sPLA2 activity was <5 nmol/minute/mL, quite similar to our observations in non-envenomed patients and those with infective cellulitis. Second, we did not observe a clear separation between sPLA2 levels of non-envenomed and envenomed patients as reported by Maduwage et al 4 Several explanations are possible why sPLA2 levels were not elevated in many of our study patients with clinically manifest envenomation.…”

Section: Discussionsupporting

confidence: 81%

Circulating Secretory Phospholipase A2 Activity following Snakebites and Its Relationship with Envenomation Status and Progression of Local Swelling

Bhardwaj

Muthu

Rajendiran

et al. 2020

The American Journal of Tropical Medicine and Hygiene

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We studied whether circulating secretory phospholipase A2 (sPLA2) activity reliably distinguished patients with snakebite envenomation from those with nonvenomous/dry snakebites, and whether patients with progressive local swelling had persistence of circulating sPLA2 activity despite antivenom treatment. We prospectively enrolled adults presenting to the emergency with a history of snakebite in the past 24 hours. We estimated circulating sPLA2 activity at baseline before antivenom administration and after 48 hours in those with envenomation. We enrolled 52 patients with snakebites (mean age 39.3 ± 12.6 years; 35 [67%] men), and 16 patients with infective cellulitis as controls. Thirty patients had local ± systemic envenomation; 15 were classified as dry/nonvenomous bites; and envenomation status was unclear in seven patients. Baseline sPLA2 activity was significantly higher in snakebite patients than that in those with infective cellulitis (4. 64 [3.38-5.91] versus 3.38 [1.69-4.01] nmol/minute/mL; P = 0.005). Among patients with snakebites, sPLA2 activity in the highest quartile was significantly associated with envenomation (12 of 27 versus two of 22; P = 0.010). However, median sPLA2 activity did not differ significantly between patients with envenomation and the rest. Baseline sPLA2 activity was significantly associated with the maximum extent of limb swelling (P = 0.031 for trend). In envenomed patients, circulating sPLA2 activity significantly decreased after 48 hours compared with the baseline (5.49 [3.38-8.86] versus 3.38 [2.53-4.64]; P = 0.003) including those with progressive swelling. Although circulating sPLA2 activity was elevated following snakebites, its sensitivity to diagnose envenomation appears to be limited. Administration of more antivenom after systemic manifestations had reversed might not benefit patients with progressive local swelling.

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Section: Discussionsupporting

confidence: 81%

Circulating Secretory Phospholipase A2 Activity following Snakebites and Its Relationship with Envenomation Status and Progression of Local Swelling

Bhardwaj

Muthu

Rajendiran

et al. 2020

The American Journal of Tropical Medicine and Hygiene

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“…Moreover, KEGG pathway enrichment analysis showed that most of the DEGs were significantly enriched in immune response pathways, such as pancreatic secretion, neuroactive ligand-receptor interaction, lysosome and protein digestion and absorption. Phospholipase A2 is a potential biomarker of inflammation that plays an important role in inflammatory diseases ( 27 ). The metal-binding protein carboxypeptidase participates in the immune response in Mice by activating or degrading signal molecules ( 28 ).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Transcriptomic and Metabolomic Analysis of the Litopenaeus vannamei Hepatopancreas After WSSV Challenge

Zhai

et al. 2022

Front. Immunol.

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Litopenaeus vannamei is the major farmed shrimp species worldwide. White spot disease due to white spot syndrome virus (WSSV) is severely affecting shrimp worldwide, causing extensive economic losses in L. vannamei culture. This is the first study that applied combined transcriptomic and metabolomic analysis to study the effects on the L. vannamei hepatopancreas after WSSV challenge. Our transcriptomic data revealed differentially expressed genes (DEGs) associated with immunity, apoptosis, the cytoskeleton and the antioxidant system in the hepatopancreas of L. vannamei. Metabolomic results showed that WSSV disrupts metabolic processes including amino acid metabolism, lipid metabolism and nucleotide metabolism. After challenged by WSSV, immune-related DEGs and differential metabolites (DMs) were detected in the hepatopancreas of L. vannamei, indicating that WSSV may damage the immune system and cause metabolic disorder in the shrimp. In summary, these results provide new insights into the molecular mechanisms underlying L. vannamei’s response to WSSV.

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Transcriptome analysis of Penaeus vannamei hepatopancreas reveals differences in toxicity mechanisms between phoxim and prometryne

Zhou

Feng

et al. 2020

Fish & Shellfish Immunology

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Tracking of secretory phospholipase A2 enzyme activity levels from childhood to adulthood: a 21-year cohort

Abstract: Childhood sPLA2 enzyme activity levels associate with adult sPLA2 levels 21 years later. Healthful changes in modifiable risk factors that occur between childhood and adulthood might prevent children from developing elevated sPLA2 levels in adulthood.

Cited by 3 publications

References 30 publications

Circulating Secretory Phospholipase A2 Activity following Snakebites and Its Relationship with Envenomation Status and Progression of Local Swelling

Circulating Secretory Phospholipase A2 Activity following Snakebites and Its Relationship with Envenomation Status and Progression of Local Swelling

Comprehensive Transcriptomic and Metabolomic Analysis of the Litopenaeus vannamei Hepatopancreas After WSSV Challenge

Transcriptome analysis of Penaeus vannamei hepatopancreas reveals differences in toxicity mechanisms between phoxim and prometryne

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