Procalcitonin as an early marker in the detection of anastomotic intestinal leak in a Universitary Hospital of Bogotá

Navarrete, Elkin Eduardo Benítez; Beltrán-García, T C; Mosquera, Maria; Rojas, Valeria Martínez; Medina, Daniel Alejandro Buitrago; Avendaño, Carlos Edgar Figueroa

doi:10.1016/j.jcol.2020.07.005

Cited by 1 publication

(1 citation statement)

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“…Sepsis is a complex and prevalent syndrome [22], the incidence of which has increased by 8.7% annually over the past 20 years [23]. Sepsis can lead to multiple organ dysfunction [24][25][26][27]. Severe war trauma and bacterial infection can also lead to sepsis [28].…”

Section: Introductionmentioning

confidence: 99%

Rapid FRET-based homogeneous immunoassay of procalcitonin using matched carbon dots labels

Liu¹,

Yang²,

Lu³

et al. 2021

Nanotechnology

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A novel method for the detection of procalcitonin in a homogeneous system by matched carbon dots (CDs) labeled immunoprobes was proposed based on the principle of FRET and double antibody sandwich method. Blue-emitting carbon dots with a strong fluorescence emission range of 400–550 nm and red-emitting carbon dots with the best excitation range of 410–550 nm were prepared before they reacted with procalcitonin protoclone antibody pairs to form immunoprobes. According to the principles of FRET, blue-emitting carbon dots were selected as the energy donor and red-emitting carbon dots as the energy receptor. The external light source excitation (310 nm) could only cause weak luminescence of CDs. However, once procalcitonin was added, procalcitonin and antibodies would be combined with each other quickly (≤20 min). Here, blue-emitting carbon dots acquired energy could be transferred to red-emitting carbon dots efficiently, causing the emitted fluorescence enhancement of red-emitting carbon dots. The fluorescence detection results in PBS buffer solution and diluted rabbit blood serum showed that the fluorescence intensity variation was linear with the concentration of procalcitonin. There was a good linear relationship between F/F0 and procalcitonin concentrations in PBS buffer solution that ranged from 0 to 100 ng ml−1, and the linear equation was F/F0 = 0.004 * C pct + 0.98359. Detection in the diluted rabbit serum led to the results that were linear in two concentration ranges, including 0–40 ng ml−1 and 40–100 ng ml−1, and the detection limit based on 3σ K−1 was 0.52 ng ml−1. It is likely that this matched CDs labeled immunoprobes system can provide a new mode for rapid homogeneous detection of disease markers.

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