Platelet antibodies identification: comparison between two laboratory tests

Dutra, Valéria de Freitas; Costa, Thiago Henrique; Santos, Leandro Dinalli; Sirianni, Marilia Fernandes Mascarenhas; Aravechia, Maria Giselda; Kutner, José Mauro; Bub, Carolina Bonet

doi:10.1016/j.htct.2020.12.008

Cited by 2 publications

(2 citation statements)

References 16 publications

(21 reference statements)

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“…Of note, our cohort size was greater. The MAIPA test may be less sensitive than the Luminex method [21]. However, classical HLA class I alloantibodies' detection from platelet lysis using the MAIPA technique (ApDIA ® ) is as sensitive as the Luminex technique [21].…”

Section: Discussionmentioning

confidence: 99%

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Low Prevalence of HLA-G Antibodies in Lung Transplant Patients Detected using MAIPA-Adapted Protocol

Pedini,

Hubert,

Carlini

et al. 2023

IJMS

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Lung transplantation is often complicated by acute and/or chronic rejection leading to graft-function loss. In addition to the HLA donor-specific antibodies (HLA-DSA), a few autoantibodies are correlated with the occurrence of these complications. Recently, antibodies directed against non-classical HLA molecules, HLA-G, -E, and -F have been detected in autoimmune diseases, like systemic lupus erythematosus. Non-classical HLA molecules are crucial in the immunological acceptance of the lung graft, and some of their isoforms, like HLA-G*01:04 and -G*01:06, are associated with a negative clinical outcome. The aim of this study is to determine the frequency of detection of HLA-G antibodies in lung transplant recipients (LTRs) and their impact on the occurrence of clinical complications. After incubating the cell lines SPI-801, with and without three different HLA-G isoform expression, with sera from 90 healthy blood donors and 35 LTRs (before and after transplantation), HLA-G reactivity was revealed using reagents from commercial monoclonal antibody immobilization of platelet antigen assay (MAIPA ApDIA®). Only one serum from one blood donor had specific reactivity against the HLA-G transduced lines. Non-specific reactivity in many sera from LTRs was observed with transduced- and wild-type cell lines, which may suggest recognition of an autoantigen expressed by the SPI-801 cell line. In conclusion, this study allowed the development of a specific detection tool for non-denatured HLA-G antibodies. These antibodies seem uncommon, both in healthy subjects and in complicated LTRs. This study should be extended to patients suffering from autoimmune diseases as well as kidney and heart transplant recipients.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The MAIPA test may be less sensitive than the Luminex method [21]. However, classical HLA class I alloantibodies' detection from platelet lysis using the MAIPA technique (ApDIA ® ) is as sensitive as the Luminex technique [21]. The HLA-G-antibody calibrated standard would allow sensitivity comparison.…”

Section: Discussionmentioning

confidence: 99%

Low Prevalence of HLA-G Antibodies in Lung Transplant Patients Detected using MAIPA-Adapted Protocol

Pedini,

Hubert,

Carlini

et al. 2023

IJMS

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Hyper-Methylation of CpG Island in 5′ UTR of the HLA-G Gene Reduces Its Expression in Individuals with Immune Thrombocytopenia

Ma,

Ning,

et al. 2024

j biomed nanotechnol

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This study investigated the impact of DNA methylation in the 5′ untranslated region-CpG island (5′ UTR) of the HLA-G gene on soluble HLA-G (sHLA-G) levels in immune thrombocytopenia (ITP) patients, shedding light on sHLA-G’s regulatory mechanisms in ITP. Using a cohort of 53 participants, including ITP patients, DNA methylation profiles in the HLA-G gene’s 5′ UTR were analyzed with Sequenom MassARRAY Methylation Analysis. sHLA-G levels were measured by enzyme-linked immunosorbent assay, and platelet antibodies were assessed using modified MAIPA. Results showed increased DNA methylation at specific CpG sites (CpG3, CpG18, CpG19, and CpG20.21) in ITP patients. A negative correlation between DNA methylation and sHLA-G expression, particularly at CpG18, was found. Patients with Anti-GPIb/IX antibodies had higher CpG18 methylation. Age and gender didn’t correlate significantly with methylation. This underscores 5′ UTR hypermethylation’s role in influencing circulating HLA-G levels, revealing insights into ITP development and potential therapeutic targets. By linking DNA methylation to sHLA-G expression, this advances ITP understanding, suggesting new therapeutic strategies.

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Platelet antibodies identification: comparison between two laboratory tests

Cited by 2 publications

References 16 publications

Low Prevalence of HLA-G Antibodies in Lung Transplant Patients Detected using MAIPA-Adapted Protocol

Low Prevalence of HLA-G Antibodies in Lung Transplant Patients Detected using MAIPA-Adapted Protocol

Hyper-Methylation of CpG Island in 5′ UTR of the HLA-G Gene Reduces Its Expression in Individuals with Immune Thrombocytopenia

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