Cytogenetic studies for cMYC, BCL2 and BCL6 genes in neoplasm: comparison of karyotype, FISH in paraffin-embedded tissue and FISH in methanol-acetic acid-fixed cells suspensions

Fluorescence in situ hybridization (FISH) in imprint of biopsies suspected of lymphoproliferative neoplasms: report on 17 cases

“… 2 , 3 Fluorescence In Situ Hybridization (FISH) technique can be applied to different types of sample preparation, such as methanol–acetic acid fixed cells suspensions, tissue imprints and FFPE. 4 , 5 The FISH technique in dewaxed tissue sections is widely used and very challenging, depending on pre-analytical processes such as fixation and paraffinization; cell overlap is also an obstacle. 6 , 7 Due to these difficulties, we propose to validate the FISH technique in imprint of biopsies from several materials suspected of having lymphoproliferative neoplasms.…”

“…Interphase FISH analysis was performed under a fluorescence microscope by two analysts with a total score of 100 nuclei, reference values previously established in bone marrow cell suspensions; IGH, CMYC, BCL2, BCL6 rearrangements cut-offs were 8.5%, 11.9%, 4.6% and 6.0%, respectively. 5 , 8 …”