Generation of hematopoietic stem/progenitor cells with sickle cell mutation from induced pluripotent stem cell in serum-free system

Paes, Bárbara Cristina Martins Fernandes; Stabeli, Luiza C.J.R.; Costa, Péricles Natan Mendes da; Orellana, Maristela Delgado; Kashima, Simone; Covas, Dimas Tadeu; Picanço‐Castro, Virgínia

doi:10.1016/j.htct.2020.01.005

Cited by 4 publications

(9 citation statements)

References 24 publications

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“…Ideally, hNPPCs derived from the hiPSCs represent a better approach to obtaining hNPPCs. Many studies have been conducted to acquire iPSCs-derived nephrogenic progenitors [ 40 ], chondroprogenitors [ 56 ], and hematopoietic progenitor cells [ 57 ] etc. In our previous study, we hypothesized that NPPCs could differentiate from iPSCs [ 58 ] and documented the differentiation protocol of hiPSCs differentiation into hNPCs [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration

Xia

Li²,

Wang³

et al. 2023

Bioactive Materials

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Section: Resultsmentioning

confidence: 99%

An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration

Xia

Li²,

Wang³

et al. 2023

Bioactive Materials

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Section: Introductionmentioning

confidence: 99%

“…Paes et al provided a simple and efficient HSC culture method using an inexpensive in-house prepared medium that is both feeder-free and serum-free [ 73 ]. They concluded that the homegrown medium produced more CD34 + CD45 + HSPCs and erythroid colonies than STEMdiff APEL 2 [ 73 ]. Moreover, Nafria et al provided a detailed procedure to generate CD45 + CD34 + RUNX1C + HSCs from spin EB that mimic intraembryonic aorta-gonad mesonephros hematopoiesis [ 59 , 74 ].…”

Section: Introductionmentioning

confidence: 99%

Recent updates of stem cell-based erythropoiesis

Han

Rim

2023

Human Cell

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Blood transfusions are now an essential part of modern medicine. Transfusable red blood cells (RBCs) are employed in various therapeutic strategies; however, the processes of blood donation, collection, and administration still involve many limitations. Notably, a lack of donors, the risk of transfusion–transmitted disease, and recent pandemics such as COVID-19 have prompted us to search for alternative therapeutics to replace this resource. Originally, RBC production was attempted via the ex vivo differentiation of stem cells. However, a more approachable and effective cell source is now required for broader applications. As a viable alternative, pluripotent stem cells have been actively used in recent research. In this review, we discuss the basic concepts related to erythropoiesis, as well as early research using hematopoietic stem cells ex vivo, and discuss the current trend of in vitro erythropoiesis using human-induced pluripotent stem cells.

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“…For many diseases, obtaining HSPCs by their mobilization using drugs is impossible due to the pathology of the diseases. Although induced pluripotent stem cells (iPSCs) generated from patients with erythroid diseases serve as a stable source of erythroid cells [10,11], this strategy is tedious and expensive. Furthermore, erythroid cells generated from iPSCs exhibit characteristics of primitive erythrocytes [12,13] with inefficient terminal maturation [14][15][16] and expression of embryonic and fetal stage proteins [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…XY,-10,der(10),add(21)(q22)[20] 16945,XY,-10,der(10),add(21)(q22)[18] PBiEPC-XY,+8,-18,+19,+21[10]/49,XY,+8,del(18)(p11.2),+19,+21[7] 181 44~48,XY,-Y,+8,-18,+19,+21[cp6] CD34iEPC 143 80~87<4n>,XXX,der(X),-3,add(4)(p13),del(4)(q21),der(4;11)(p13;q23),der(5;11)(q35;q23),-7, add(7)(p14), der(8;15)(p23;p10),-9, add(9)(q34),der(10),-12, der(13;15)(p10;p10),-14,-17, i(17)(q10), add(21)(p10), add(21)(q21), +mar[cp20] 157 79~92<4n>,XXX,der(X),-3,add(6)(p23),-7,t(7;10)(q32;q24),add(8)(p27), der(8),-9, -10,-11,-11,-12, der(13), -14,-15,der(15),-16,der(17),i(17)(q10),der(21),+mar,+mar[cp20]…”

mentioning

confidence: 99%

Direct Generation of Immortalized Erythroid Progenitor Cell Lines from Peripheral Blood Mononuclear Cells

Bagchi

Nath

Thamodaran

et al. 2021

Cells

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Reliable human erythroid progenitor cell (EPC) lines that can differentiate to the later stages of erythropoiesis are important cellular models for studying molecular mechanisms of human erythropoiesis in normal and pathological conditions. Two immortalized erythroid progenitor cells (iEPCs), HUDEP-2 and BEL-A, generated from CD34+ hematopoietic progenitors by the doxycycline (dox) inducible expression of human papillomavirus E6 and E7 (HEE) genes, are currently being used extensively to study transcriptional regulation of human erythropoiesis and identify novel therapeutic targets for red cell diseases. However, the generation of iEPCs from the patients with red cell diseases is challenging as obtaining a sufficient number of CD34+ cells require bone marrow aspiration or their mobilization to peripheral blood using drugs. This study established a protocol for culturing early-stage EPCs from peripheral blood (PB) and their immortalization by expressing HEE genes. We generated two iEPCs, PBiEPC-1 and PBiEPC-2, from the peripheral blood mononuclear cells (PBMNCs) of two healthy donors. These cell lines showed stable doubling times with the properties of erythroid progenitors. PBiEPC-1 showed robust terminal differentiation with high enucleation efficiency, and it could be successfully gene manipulated by gene knockdown and knockout strategies with high efficiencies, without affecting its differentiation. This protocol is suitable for generating a bank of iEPCs from patients with rare red cell genetic disorders for studying disease mechanisms and drug discovery.

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Generation of hematopoietic stem/progenitor cells with sickle cell mutation from induced pluripotent stem cell in serum-free system

Cited by 4 publications

References 24 publications

An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration

An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration

Recent updates of stem cell-based erythropoiesis

Direct Generation of Immortalized Erythroid Progenitor Cell Lines from Peripheral Blood Mononuclear Cells

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