Use of the reticulocyte channel warmed to 41 °C of the XN-9000 analyzer in samples with the presence of cold agglutinins

Roccaforte, Vincenzo; Sciarini, Flavia; Proserpio, Vanessa; Buonocore, Ruggero; Zavaroni, Emanuela; Burati, Silvia; Bussetti, Marco; Liuzzi, Giammaria; Russo, Rosalba; Porreca, Wanda Patrizia; Angelis, Maria Luisa De; Perno, Carlo Federico; Bonato, Claudio; Pastori, Stefano

doi:10.1016/j.htct.2020.01.004

Cited by 5 publications

(10 citation statements)

References 12 publications

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“…Many interferences are described in the literature [1][2][3][4][5][6][7][8][9][10] ; however, some of them were not included in our study, for the following reasons:…”

Section: Interferences Groupsmentioning

confidence: 99%

“…Several sources of interferences are well-recognized: plasma changes (due to hemolysis, lipemia, icteric plasma, or immunoglobulins), haemoglobin or red blood cells disorders, leukocytosis. [1][2][3][4][5][6][7] With the development of new generation analyzers, novel technologies and parameters have been introduced to improve both analytical performances and detection of presumed interferences.…”

Section: Introductionmentioning

confidence: 99%

“…While many situations may impair measurements and accurate cell counts, interferences affecting red blood cells (RBCs) and the associated parameters are among the most common. Several sources of interferences are well‐recognized: plasma changes (due to hemolysis, lipemia, icteric plasma, or immunoglobulins), haemoglobin or red blood cells disorders, leukocytosis 1–7 …”

Section: Introductionmentioning

confidence: 99%

“…Regarding interferences with XN‐10, the circumstances associated with spurious increased mean corpuscular haemoglobin concentration (MCHC), which stands as one of the best identified and described interference‐related situation, have been studied by Berda‐Haddad et al who proposed a decision‐tree to ensure accurate results 1 and by Roccaforte et al as well who studied cold agglutinins and RET channel especially 3 ; in addition, Aruga et al have recently assessed haemoglobin concentration in case of chylous turbidity 2 . Furthermore, the impact of leukocytosis on RBCs parameters and the subsequent management has been recently evaluated by Schillinger 4 …”

Section: Introductionmentioning

confidence: 99%

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Optimizing the management of analytical interferences affecting red blood cells on XN‐10 (Sysmex®)

Henry

Gérard

Salignac³

et al. 2022

Int J Lab Hematology

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Introduction: Interferences on red blood cells (RBCs) measurement and the associated parameters in haematology analyzers are very common. Many sources of interferences are described but their management remains uncertain depending on the measurement system; we aimed at developing an optimized scheme allowing the accurate management of most interferences affecting RBCs, based on the alternative "optical" parameters from SYSMEX XN-10.Methods: Samples from 12 groups of relevant interferences were analysed and compared with a control group allowing (1) the determination of deviation thresholds beyond which an interference is likely, and (2) the development of two flowcharts for their subsequent management. These flowcharts were then evaluated among a bank of retrospective typical cases of interferences and in the routine flow of the laboratory.Results: After verifying the excellent agreement between standard and alternative parameters, the comparative study between analytical channels allowed to determine an acceptable deviation and then discriminate technical concerns caused by cold agglutinins, leukocytosis and plasma-related interferences. This led to the development of flowcharts ensuring the accurate management of these interferences, whether MCHC is <320 or >365 g/L. These proposed flowcharts allowed the correction of 63/65 historical confirmed interferences cases (97%). Furthermore, they corrected 18 results among 901 unselected prospective samples. Conclusion:The resulting flowcharts allow a relevant correction for most common interferences affecting RBCs and are now definitively included in the routine analytical management and will be directly incorporated in the middleware of the laboratory.

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“…Many interferences are described in the literature [1][2][3][4][5][6][7][8][9][10] ; however, some of them were not included in our study, for the following reasons:…”

Section: Interferences Groupsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Optimizing the management of analytical interferences affecting red blood cells on XN‐10 (Sysmex®)

Henry

Gérard

Salignac³

et al. 2022

Int J Lab Hematology

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“…Recent studies have suggested that the Sysmex XN reticulocyte channel can produce reliable RBC parameters except for MCHC for samples containing CAs, which is significantly helpful for rapid correction of RBC count. 9,10 For samples containing high-titer CAs, it is still worth exploring how to obtain accurate results.…”

mentioning

confidence: 99%

Effect of preheating at 40℃ for 15 minutes to high‐titer cold agglutinins on complete blood count

Xie

et al. 2021

Int J Lab Hematology

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Cold agglutinins (CAs) are a type of red cell autoantibody, mostly comprised of IgM and a few IgG and IgA. 1 At temperatures below 31°C, these antibodies easily combine with I, i, or SP1 antigens on red cells, resulting in red cell agglutination and microcirculation blockage, which could cause cyanosis and even autoimmune hemolytic anemia (AIHA) in more severe cases. 2 AIHA caused by CAs can be divided into two types: cold agglutinin disease (CAD) and cold agglutinin syndrome (CAS). The former is B-cell clonal lymphoproliferative disorder (LPD); the latter is commonly seen in mycoplasma pneumonia, infectious mononucleosis, and lymphoma. [2][3][4] High concentrations of CA can induce erythrocyte agglutination, which has a great impact on complete blood count (CBC) results. In

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Effects of cold agglutinins on the automated complete blood count accuracy

Gioia¹

2021

Scandinavian Journal of Clinical and Laboratory Investigation

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Use of the reticulocyte channel warmed to 41 °C of the XN-9000 analyzer in samples with the presence of cold agglutinins

Cited by 5 publications

References 12 publications

Optimizing the management of analytical interferences affecting red blood cells on XN‐10 (Sysmex®)

Optimizing the management of analytical interferences affecting red blood cells on XN‐10 (Sysmex®)

Effect of preheating at 40℃ for 15 minutes to high‐titer cold agglutinins on complete blood count

Effects of cold agglutinins on the automated complete blood count accuracy

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