Stability of refrigerated whole blood samples for osmotic fragility test

Salvagno, Gian Luca; Demonte, Davide; Dima, Francesco; Bovo, Chiara; Lippi, Giuseppe

doi:10.1016/j.htct.2019.06.001

Cited by 6 publications

(8 citation statements)

References 10 publications

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“…However, the mean value at the end of the storage was still to be considered as normal in all type of bags. The blood sample storage conditions could have a strong influence on erythrocyte shape, volume, and integrity because of the continuous anaerobic metabolism of these blood elements, leading to increased vulnerability at a relatively high osmotic strength 28 . In a recent study, the MOF of RBCs increased constitutively during storage, but it was significantly lower in WB than in pRBC units, throughout the storage period 30…”

Section: Discussionmentioning

confidence: 98%

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Effects of leukoreduction on storage lesions in whole blood and blood components of dogs

Stefani

Capello

Carminato³

et al. 2021

Veterinary Internal Medicne

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Background: Leukoreduction is a routine procedure in human transfusion medicine but is uncommon in veterinary. Objectives: To evaluate the effect of leukoreduction on the quality of canine whole blood (WB) and blood products during storage. Animals: Ten canine blood donors. Methods: This is a case series study. An amount of 450 mL of blood was collected from each dog. Five WB and 5 packed red blood cells (pRBC) bags were divided into 2 units each: leukoreduced (LR) and non-leukoreduced (nLR). RBC count, erythrocytes' mean osmotic fragility (MOF), 2,3-diphosphoglycerate (2,3-DPG), adenosine triphosphate (ATP), percentage of hemolysis, potassium (K), lactate, glucose, and cytokines were measured weekly from day of donation (T0) to day 35 (T35); pH, coagulation times, and clotting factors were evaluated at T0 and T35 from WB and in fresh frozen plasma after 1 year of storage.

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Section: Discussionmentioning

confidence: 98%

“…The osmotic fragility test is conventionally used to assess erythrocyte resistance to hemolysis, using a series of hypotonic solutions of NaCl 28 . In healthy animals, the MOF (corresponding at the NaCl concentration at which 50% of RBCs lyzed) occurs around 0.5% NaCl 29 .…”

Section: Discussionmentioning

confidence: 99%

Effects of leukoreduction on storage lesions in whole blood and blood components of dogs

Stefani

Capello

Carminato³

et al. 2021

Veterinary Internal Medicne

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“…2 It is used for diagnosis of several erythrocyte disorders, such as HS, elliptocytosis, and thalassemias. 3 Recently, new methods have been developed for diagnosis of HS including the flow cytometry. 4 However, flow cytometers are not available in all routine diagnostic laboratories.…”

mentioning

confidence: 99%

“…The osmotic fragility test is a labor-intensive and time-consuming test to perform and has a low sensitivity and specificity 2. It is used for diagnosis of several erythrocyte disorders, such as HS, elliptocytosis, and thalassemias 3. Recently, new methods have been developed for diagnosis of HS including the flow cytometry 4.…”

mentioning

confidence: 99%

Clinical Features and Outcome of Children With Hereditary Spherocytosis

Kiliç¹,

Özdemir

Tahtakesen

et al. 2021

Journal of Pediatric Hematology/Oncology

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Objective: The aim of this study was to evaluate the demographics, clinical, and laboratory findings and treatment responses of patients with hereditary spherocytosis (HS).Materials and Methods: Data of children with HS were examined. Diagnosis was based on clinical history, physical examination, family history, presence of spherocytes on peripheral blood smear, and osmotic fragility test.Results: A total of 101 patients were included. The median (range) age at diagnosis was 38.0 (1 to 188) months. Mild, moderate, and severe forms of HS were present in 29 (28.7%), 15 (14.9%), and 57 (56.4%) patients, respectively. Family history was available in 73 patients and 56 of these (76.7%) had a positive family history for HS. Forty-five (44.5%) patients needed regular transfusions and all of these had severe disease. Although most patients did not require transfusion postsplenectomy, 2 of 45 (4.4%) patients continued to require transfusion. Transfusion dependence was significantly (P < 0.001) higher in patients with severe spherocytosis. Conclusions:In HS, splenomegaly, pallor, and jaundice are the most common clinical features. Splenectomy dramatically reduces hemolysis in most cases and virtually abolishes further requirement for transfusion.

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“…Uma vez que o tempo e a própria temperatura de armazenamento podem afetar a estabilidade de eritrócitos 125 , é relevante ter estas variáveis sob controle, embora um armazenamento da amostra de sangue entre 4 e 8 C por até 4 dias não tenha afetado as constantes de estabilidade osmótica aqui consideradas 126 .…”

Section: A Estabilidade Osmótica De Eritrócitosunclassified

Amplitude da distribuição de volume (RDW) e da estabilidade osmótica (dX) de eritrócitos no diabetes mellitus tipo 2

Knychala¹

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Stability of refrigerated whole blood samples for osmotic fragility test

Cited by 6 publications

References 10 publications

Effects of leukoreduction on storage lesions in whole blood and blood components of dogs

Effects of leukoreduction on storage lesions in whole blood and blood components of dogs

Clinical Features and Outcome of Children With Hereditary Spherocytosis

Amplitude da distribuição de volume (RDW) e da estabilidade osmótica (dX) de eritrócitos no diabetes mellitus tipo 2

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