Differential expression patterns of two new primary cell wall-related cellulose synthase cDNAs, PtrCesA6 and PtrCesA7 from aspen trees

Samuga, Anita; Joshi, Chandrashekhar P.

doi:10.1016/j.gene.2004.02.057

Cited by 29 publications

(22 citation statements)

References 27 publications

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“…In the same way, PtrCesA1, PtrCesA2, and PtrCesA3 are involved with cellulose deposition in the secondary cell wall and PtrCesA4, PtrCesA5, PtrCesA6, and PtrCesA7 may be involved with cellulose deposition in the primary cell wall Kalluri and Joshi, 2004;Samuga and Joshi, 2004b). The gene AtCesA8 present in the lew2-1 mutant genome was further characterized and its disruption increases osmotic and drought stress tolerance, indicating that cellulose synthesis may be involved in stress tolerance (Chen et al, 2005), but the cause and consequence relationship needs to be delineated.…”

Section: Cesa Gene Family In Arabidopsis Thaliana and Populus Tremulomentioning

confidence: 97%

“…The genome of aspen has at least nine types of CesA genes: PtrCesA1 (Wu et al, 2000), PtrCesA2 (Samuga and Joshi, 2002), PtrCesA3 , PtrCesA4 , PtrCesA5 (Kalluri and Joshi, 2003), PtrCesA6 (Samuga and Joshi, 2004b), and PtrCesA7 (Samuga and Joshi, 2004b) all of which encode distinct CESAs. In addition, two partial CSR regions have been isolated that are designated PtrCesA8 and PtrCesA9 .…”

Section: Cesa Gene Family In Arabidopsis Thaliana and Populus Tremulomentioning

confidence: 99%

“…Protein sequence comparisons of higher plant CESAs reveal that all CESAs are grouped into six distinct clades which contain members associated with the primary or secondary cell wall ( Figure 6) (Holland et al, 2000;Vergara and Carpita, 2001;Joshi et al, 2004;Liang and Joshi, 2004;Samuga and Joshi, 2004b;Nairn and Haselkorn, 2005;Somerville, 2006). In fact, orthologs of AtCesA1, AtCesA3, and AtCesA6 appear to be required for the synthesis of cellulose deposited in the primary cell walls (Fagard et al, 2000;Scheible et al, 2001;Burn et al, 2002;Doblin et al, 2002).…”

Section: Cesa Gene Family In Arabidopsis Thaliana and Populus Tremulomentioning

confidence: 99%

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Structure, organization, and functions of cellulose synthase complexes in higher plants

Festucci-Buselli

Otoni

Joshi

2007

Braz. J. Plant Physiol.

111

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Annually, plants produce about 180 billion tons of cellulose making it the largest reservoir of organic carbon on Earth. Cellulose is a linear homopolymer of beta(1-4)-linked glucose residues. The coordinated synthesis of glucose chains is orchestrated by specific plasma membrane-bound cellulose synthase complexes (CelS). The CelS is postulated to be composed of approximately 36 cellulose synthase (CESA) subunits. The CelS synthesizes 36 glucose chains in close proximity before they are further organized into microfibrils that are further associated with other cell wall polymers. The 36 glucose chains in a microfibril are stabilized by intra- and inter-hydrogen bonding which confer great stability on microfibrils. Several elementary microfibrils come together to form macrofibrils. Many CESA isoforms appear to be involved in the cellulose biosynthetic process and at least three types of CESA isoforms appear to be necessary for the functional organization of CelS in higher plants.

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Section: Cesa Gene Family In Arabidopsis Thaliana and Populus Tremulomentioning

confidence: 97%

Section: Cesa Gene Family In Arabidopsis Thaliana and Populus Tremulomentioning

confidence: 99%

Section: Cesa Gene Family In Arabidopsis Thaliana and Populus Tremulomentioning

confidence: 99%

See 1 more Smart Citation

Structure, organization, and functions of cellulose synthase complexes in higher plants

Festucci-Buselli

Otoni

Joshi

2007

Braz. J. Plant Physiol.

111

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“…In plants there are two distinct CesA gene expression groups, one associated with primary cell wall formation and the other with secondary cell wall formation (Taylor et al 2004;Desprez et al 2007). Independent functional analyses of the CesA genes in woody and herbaceous species have revealed that orthologous CesA genes are functionally conserved in diverse plant species (Tanaka et al 2003;Taylor et al 2003;Samuga and Joshi 2004;Ranik and Myburg 2006;Kumar et al 2009). This conservation likely includes a set of shared cisregulatory sequences and transcription factors since CesA gene expression profiles are also highly conserved across different plant genera (Burton et al 2004;Ranik and Myburg 2006;Creux et al 2008).…”

Section: Eucalyptusmentioning

confidence: 99%

Diversity and cis-element architecture of the promoter regions of cellulose synthase genes in Eucalyptus

Creux

Castro

Ranik

et al. 2013

Tree Genetics & Genomes

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Lignocellulosic biomass from fast-growing plantation trees is composed of carbohydrate-rich materials deposited into plant cell walls in a coordinated manner during wood formation. The diversity and evolution of the transcriptional networks regulating this process have not been studied extensively. We investigated patterns of species-level nucleotide diversity in the -2 -promoters of cellulose synthase (CesA) genes from different Eucalyptus tree species and assessed the possible roles of DNA sequence polymorphism in the gain or loss of ciselements harboured within the promoters. Promoter regions of three primary and three secondary cell wall-associated CesA genes were isolated from 13 Eucalyptus species and were analysed for nucleotide and cis-element diversity. Species-level nucleotide diversity (π) ranged from 0.014 to 0.068 and different CesA promoters exhibited distinct patterns of sequence conservation. A set of 22 putative cis-elements were mapped to the CesA promoters using in silico methods. Forty two percent of the mapped cis-element occurrences contained singleton polymorphisms which resulted in either the gain or loss of a cis-element in a particular Eucalyptus species. The promoters of Eucalyptus CesA genes contained regions that are highly conserved at the species (Eucalyptus) and genus (with Arabidopsis and Populus) level, suggesting the presence of regulatory modules imposing functional constraint on such regions. Nucleotide polymorphisms in the CesA promoters more frequently created new cis-element occurrences than disrupted existing cis-element occurrences, a process which may be important for the maintenance and evolution of cellulose gene regulation in plants.

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“…A phylogenetic tree developed on the basis of protein similarities among 52 CesAs indicated that higher plant CesAs could be divided into six groups, three primary and three secondary CesAs [27]. In Arabidopsis mutant studies, AtCesA1, AtCesA3, and AtCesA6 have been shown to be required for the cellulose synthesis in primary cell wall [1,7,12,[28][29][30][31], indicating the potential of their interactions with each other.…”

Section: Interactions Among Primary Cell Wall-specific Cellulose Syntmentioning

confidence: 99%

In vitro demonstration of interactions among zinc-binding domains of cellulose synthases in Arabidopsis and aspen

Xu¹,

Joshi²

2010

ABB

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Plant cellulose synthases (CesAs) are the key enzymes necessary for cellulose biosynthesis. In Arabidopsis, two distinct groups of three CesAs each are necessary for cellulose synthesis during primary and secondary cell wall formation. It has also been suggested that such three CesAs interact with each other to form plasma-membrane bound rosette complexes that are functional during cellulose production. However, in vivo demonstration of such assemblies of three CesAs into rosettes has not been possible. We used yeast two-hybrid assays to demonstrate the possible interactions among several CesAs from Arabidopsis and aspen via their N-terminal zinc-binding domains (ZnBDs). While strong positive interactions were detected among ZnBDs from secondary wall associated CesAs of both Arabidopsis and aspen, the intergeneric interactions between Arabidopsis and aspen CesAs were weak. Moreover, in aspen, three primary wall associated CesA ZnBDs positively interacted with each other as well as with secondary CesAs. These results suggest that ZnBDs from either primary or secondary CesAs, and even from different plant species could interact but are perhaps insufficient for specificities of such interactions among CesAs. These observations suggest that some other more specific interacting regions might exist within CesAs. It is also possible that some hitherto unknown mechanism exists in plants for assembling the rosette complexes with different compositions of CesAs. Understanding how cellulose is synthesized will have a direct impact on utilization of lignocellulosic biomass for bioenergy production.

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Differential expression patterns of two new primary cell wall-related cellulose synthase cDNAs, PtrCesA6 and PtrCesA7 from aspen trees

Cited by 29 publications

References 27 publications

Structure, organization, and functions of cellulose synthase complexes in higher plants

Structure, organization, and functions of cellulose synthase complexes in higher plants

Diversity and cis-element architecture of the promoter regions of cellulose synthase genes in Eucalyptus

In vitro demonstration of interactions among zinc-binding domains of cellulose synthases in Arabidopsis and aspen

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